Accu check active glucose monitor

To induce the diabetes mellitus experimental model, 66 animals were injected via single intraperitoneal injection with STZ (50 mg/kg) freshly prepared in a cold 0.1 M sodium citrate buffer (pH 4.5) prior to fasting for approximately 16 h, with only water provided ad libitum. The rats in the normal group were injected intraperitoneally with sodium citrate buffer (pH 4.5) [18 , 19 (link)]. Three days after induction, a drop of blood (~ 1.5 μl) from the tail vein of each rat was obtained using a lancet. The blood was tested using a commercial glucometer (ACCU-CHECK Active Glucose Monitor, Roche, Germany) to determine hyperglycemia development. The fasting rats with a blood glucose level > 11 mmol/L on day 7 after injection were considered diabetic and were used in further experiments in this study [20 ].

After overnight fasting, diabetes mellitus was induced in animals with a single intraperitoneal injection (i.p.) of streptozotocin (STZ) at 55 mg/kg prepared in a 0.01M citrate buffer of 4.5 pH [21 (link)]. On the third day after STZ injection, a drop of blood was drawn from the tail vein of each rat, and the blood glucose levels were estimated using a glucometer (ACCUCHECK Active Glucose Monitor, Roche, Germany). Rats with blood glucose levels of ≥250 mg/dL were considered diabetic and underwent surgical wounding.