Anti hla dr g46 6

Manufactured by BD

Anti-HLA-DR (G46-6) is a monoclonal antibody that recognizes the human leukocyte antigen (HLA) class II molecule HLA-DR. It is a useful tool for immunophenotyping and can be used to identify and enumerate HLA-DR-expressing cells in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Epics xl mcl flow cytometer, by Beckman Coulter (1 mentions) Anti cd27 o323, by BioLegend (1 mentions) Anti cd3 sp34 2, by BD (1 mentions) Anti cd4 l200, by BD (1 mentions) Anti cd3 ucht1, by BD (1 mentions) Anti cd8 sk1, by BD (1 mentions) Anti cd19 hib19, by BD (1 mentions) Anti cd123 7g3, by BD (1 mentions) Anti cd38 hit2, by BD (1 mentions) Anti cd14 m5e2, by BD (1 mentions) Compensation beads, by BD (1 mentions) Natural der p 1, by Indoor Biotechnologies (1 mentions) Aqua viability dye, by Thermo Fisher Scientific (1 mentions) Rabbit anti human ige antibody, by Fortis Life Sciences (1 mentions) Facs lysing solution, by BD (1 mentions)

3 protocols using anti hla dr g46 6

Immune Cell Phenotyping under BSL 2+

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immune cell phenotyping was performed under BSL 2+ conditions by incubating 200 μL of fresh whole blood in polystyrene tubes with two different fluorochrome-labeled antibody panels for 20 min in the dark [Panel 1: anti-CD3 (SP34-2, #562877), anti-CD4 (L200, #560836), anti-CD8 (SK1, #341051); anti-CD19 (HIB19, #555415), anti-CD38 (HIT2, #555460), and anti-HLA-DR (G46-6, #555811) from BD, anti-CD20 (2H7, #47-0209-42) from eBioscience, and anti-CD27 (O323, #302838) from BioLegend. Panel 2: anti-CD3 (UCHT1, #557943), anti-CD11c (O33-782, 561355), anti-CD14 (M5E2, #565283), anti-CD19 (HIB19, #557921), anti-CD123 (7G3, #554529), and anti-HLA-DR (G46-6, #560651) from BD; anti-CD16 (CB16, #47-1068) and anti-CD56 (MEM188, #17-0569) from eBioscience, and anti-CD20 (2H7, #302332) from BioLegend]. Flow cytometry was performed on an LSRII (BD) and data was analyzed using FlowJo software version 9 (Tree Star). T cells expressing both HLA-DR and CD38 were considered activated.

Raabe V., Lai L., Xu Y., Huerta C., Wang D., Pouch S.M., Burke C.W., Piper A.E., Gardner C.L., Glass P.J, & Mulligan M.J. (2020). The Immune Response to Eastern Equine Encephalitis Virus Acquired Through Organ Transplantation. Frontiers in Microbiology, 11, 561530.

+ Open protocol

+ Expand

Phenotypic Profiling of Activated T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

All antibodies were mouse anti-human monoclonal antibodies (mAb). PE-Cy5-conjugated anti-CD4 (OKT4) were from Beckman Coulter (Miami, FL). PE-conjugated anti-PD-1 (MIH4), fluorescein isothiocyanate (FITC)-conjugated anti-CD25 (M-A251), anti-CD69 (FN50), and anti-HLA-DR (G46-6) were from BD PharMingen (Franklin Lakes, NJ). PE- or PE-Cy5-conjugated IgG1 (679.1Mc7) (Beckman Coulter), PE-conjugated IgG2a (eBM2a; eBio-science), and FITC-conjugated IgG1 (P3) (eBioscience) were used as IgG isotype controls. PBMCs (0.5 × 10⁶) were incubated in wash buffer (PBS/2.5% fetal bovine serum [FBS]) with appropriate amounts of mAb on ice for 30 minutes. Cells were washed and were immediately analyzed on an EPICS XL-MCL flow cytometer (Beckman Coulter).

Liu C., Jiang J., Gao L., Hu X., Wang F., Shen Y., Yu G., Zhao Z, & Zhang X. (2014). A Promoter Region Polymorphism in PDCD-1 Gene Is Associated with Risk of Rheumatoid Arthritis in the Han Chinese Population of Southeastern China. International Journal of Genomics, 2014, 247637.

+ Open protocol

+ Expand

Allergen and Rhinovirus Detection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Allergens: Purified allergens (natural Der p 1, natural Der p 2, and recombinant H22-Fel d 1) with low endotoxin content (<25 IU/μg) were obtained from Indoor Biotechnologies, Inc. (Charlottesville, VA). Rhinovirus Preparation: FDA-approved RV-16 pool was kindly provided by Dr. Ronald Turner (University of Virginia). Flow Cytometry Antibodies: Fluorochrome-labeled monoclonal antibodies used for flow cytometry were: Lin3 cocktail (anti-CD3, -CD14, -CD19 and -CD20), Lin1 cocktail (anti-CD3, -CD14, -CD16, -CD19, -CD20 and -CD56), anti-CD123 (clone 6H6), and anti-HLA-DR (G46-6) purchased from BD Biosciences (San Jose, CA); anti-TSLPR (1B4), anti-CD1c (L161), anti-CD63 (H5C6) and anti-CD203c (NP4D6) (Biolegend, San Diego, CA); anti-CD11c (B-ly6) and anti-FcεRIα (CRA–1) (eBiosciences, San Diego, CA); and anti-Syk (4D10.1) (EMD Millipore, Billerica, MA). Compensation beads were purchased from BD Biosciences. Aqua viability dye was used to determine cell viability (Invitrogen, Carlsbad, CA). Other Reagents: Mouse anti-FcεRIα monoclonal antibody (clone AER-37) was purchased from Biolegend and rabbit anti-human IgE antibody was obtained from Bethyl Laboratories, Inc. (Montgomery, TX). BD FACS lysing solution for fresh whole blood staining was purchased from BD Biosciences.

Agrawal R., Wisniewski J., Yu M.D., Kennedy J.L., Platts-Mills T., Heymann P.W, & Woodfolk J.A. (2014). Infection with Human Rhinovirus 16 Promotes Enhanced IgE Responsiveness in Basophils of Atopic Asthmatics. Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 44(10), 1266-1273.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!