Ms grade acetonitrile

De Man Rogosa and Sharpe broth/agar (MRS), plate count agar, rose Bengal agar, and yeast extract were purchased from Guangdong Huankai Microbiology Technology Co., Ltd. (Guangzhou, China). Polypeptone, glucose fructose, sucrose, acetic acid, citric acid, lactic acid, malic acid, oxalic acid, and L-ascorbic acid were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). 1-Kestose, nystose and 1F-fructofuranosylnystose were obtained from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). Mass spectrometry (MS)-grade acetonitrile, cyclohexanone, and methanol were obtained from Merck (Darmstadt, Germany). Pure water was obtained using a Milli-Q system (Bedford, MA, USA). Phenol and acetocaustin were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Other reagents were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

QXH (Lot. 161030) was supplied by Shaanxi Momentum Pharmaceutical Co., Ltd., Shaanxi, China; compound danshen dripping pills (CDDP, Lot. 180602) were purchased from Tasly Pharmaceutical Co., Ltd., Tianjin, China; and adrenaline hydrochloride injection (Adr, Lot. 20190102) was obtained from Grand Pharmaceutical Co., Ltd., Wuhan, China. Carboxymethylcellulose sodium (Lot. 20180803) was purchased from Tianli Chemical Reagent Co., Ltd., Tianjin, China; and disposable human venous blood sample collection containers (3.2% sodium citrate anticoagulation, blood/citrate: 9 : 1, v/v, Lot. 20190407, and heparin sodium anticoagulation, Lot. 20190705) were obtained from Yuli Medical Instrument Co., Ltd., Jiangsu, China. PT (Lot. 20190214), APTT (Lot. 20190214), TT (Lot. 20190114), and FIB (Lot. 20192012) assay kits were obtained from Steellex Biotechnology Co., Ltd., Taizhou, China. MS-grade acetonitrile and methanol were purchased from Merck, Darmstadt, Germany. MS-grade formic acid was obtained from Sigma-Aldrich, Darmstadt, Germany. Water used in the experiment was purified by using a Milli-Q water purification system (Billerica, MI, USA). Reference substances were purchased from Yuanye Bio-Technology Co., Ltd., Shanghai, China. The purity of each compound was determined to be higher than 98% by HPLC.

SRL and cyclosporin D (internal standard, IS) (Figure 1) with analytical reference standards (purity ≥ 99.0%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). SRL tablets (1 mg per tablet) were obtained from Wyeth Company (USA). TRQ injections (10 mL per vial) were provided from Shanghai Kaibao Pharmaceutical Co., Ltd (China). MS-grade acetonitrile and methanol were purchased from Merck (Darmstadt, Germany). Deionized water was prepared using the Milli-Q system (Millipore, Bedford, MA, USA) and was used for all preparations. Other reagents were of analytical grade.
Stock solutions (1 mg/mL) of SRL and IS were prepared in methanol, respectively. SRL stock solution was diluted in methanol-water (50:50) to obtain a series of working solutions, at concentrations of 25, 50, 100, 200, 400, 500, 800, and 1000 ng/mL. Calibration standards were prepared by dilutions of the above working solutions with appropriate blank rat blood to attain concentrations of 2.5, 5, 10, 20, 40, 50, 80, and 100 ng/mL. QC samples were obtained with concentrations of 5, 40, and 80 ng/mL. All solutions were stored at −20°C.

Three roots with similar morphological characteristics were randomly selected for sampling. In all, 71 samples of A. tanguticus were collected at altitudes ranging from 2,700 m to 4,100 m, longitude ranging from 95° to 102°, and latitudes ranging from 30° to 38° in August 2020 in Qinghai–Tibet Plateau, China (Figure 1). The sampling sites basically covered the entire geographic distribution area of A. tanguticus according to the specimen information of A. tanguticus was collected from the NSII China National Specimen Resource Platform (http://www.nsii.org.cn/) and China Digital Herbarium (https://www.cvh.ac.cn/). MS grade acetonitrile and formic acid were obtained from Merck.

The reference standards of gefitinib (Product number: Y0001809, Lot number: BCCB6739), MS-grade acetonitrile (Product number: 1.00029.2500, Lot number: I1066929001), and formic acid (Product number: 94318-250ML-F, Lot number: BCBN7383V) for UPLC-Q-TOF-MS/MS analysis were purchased from Merck Company (Darmstadt, Germany). Deionized water was freshly processed through a Milli-Q water purification system (Millipore, USA).