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Anti ly51

Manufactured by BioLegend

Anti-Ly51 is a monoclonal antibody that recognizes the Ly51 antigen, also known as ER-TR9. Ly51 is a cell surface marker expressed on a subset of stromal cells in the thymus and bone marrow.

Automatically generated - may contain errors

2 protocols using anti ly51

1

Immunofluorescence Imaging of Thymic Tissue

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Paraformaldehyde-fixed frozen tissues embedded in OCT compound (Sakura Finetek) were sliced into 10-μm-thick sections. The sections were stained with anti-CCL21 (Bio-Rad), anti-Aire (eBioscience), anti-Ly51 (Biolegend), anti-Krt14 (Biolegend), anti-DCLK1 (abcam), anti-CD4 (Biolegend), anti-CD8 (Biolegend) antibodies, anti-Foxn1 antiserum50 (link), and UEA-1 (Vector Laboratories). Images were analyzed with a TCS SP8 confocal laser scanning microscope (Leica).
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2

Immunofluorescence Analysis of Thymic Microenvironment

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Paraformaldehyde-fixed frozen tissues embedded in OCT compound (Sakura Finetek) were sliced into 10-μm-thick sections. The sections were stained with anti-CCL21 (Bio-Rad, Cat# AAM27, RRID:AB_2072089), anti-Aire (eBioscience, Cat# 50593482, RRID:AB_2574257), anti-Ly51 (BioLegend, Cat# 108312, RRID:AB_2099613), anti-Krt14 (BioLegend, Cat# 905304, RRID:AB_2616896), anti-DCLK1 (abcam, Cat# ab31704, RRID:AB_873537), anti-CD4 (BioLegend, Cat# 100533, RRID:AB_493372), anti-CD8 (BioLegend, Cat# 100701, RRID:AB_ 312740) antibodies, anti-Foxn1 antiserum (Itoi et al., 2007 (link)), and UEA-1 (Vector Laboratories, Cat# B1065, RRID:AB_2336766). Images were analyzed with a TCS SP8 confocal laser scanning microscope (Leica).
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