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Rta00

Manufactured by R&D Systems
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Sourced in United States

The RTA00 is a laboratory equipment product designed for research applications. It serves as a tool for performing various experimental tasks within a controlled laboratory environment. The core function of the RTA00 is to facilitate the execution of research protocols and the gathering of data, without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Rlb00, by R&D Systems (15 mentions) Eril1b, by R&D Systems (3 mentions) R1000, by R&D Systems (3 mentions) Radioimmunoprecipitation assay (ripa), by Merck Group (2 mentions) Advia 2120i, by Siemens (2 mentions) Tba 120fr, by Toshiba (2 mentions) Srlb00, by R&D Systems (2 mentions) Bca protein assay, by Thermo Fisher Scientific (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Mrt10, by R&D Systems (1 mentions) Assay kits for glutathione, by Cayman Chemical (1 mentions) Ab100778, by Abcam (1 mentions) Dbnt00, by R&D Systems (1 mentions) Csb e08211r, by Cusabio (1 mentions) Macs octo dissociator, by Miltenyi Biotec (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Multiskan fc with incubator 51119100, by Thermo Fisher Scientific (1 mentions) Quantikine elisa, by R&D Systems (1 mentions) Hs600b, by R&D Systems (1 mentions) Softmax pro 6, by Molecular Devices (1 mentions)

42 protocols using rta00

1

Serum Cytokine Profiling in Rats

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Serum was collected monthly from the control and treated rats, and inflammatory cytokines were assessed by enzyme-linked immunosorbent assays (ELISAs) using rat ELISA kits according to the manufacturers' protocols. For rat TNF-α and TNF receptor 1 (TNFR1), catalog #RTA00 and MRT10 from R & D Systems (Minneapolis, MN) were used, respectively; for interleukin-8 (IL-8) catalog #R6417 from Biotang Inc. (Waltham, MA); for the S100A8/A9 complex catalog #MBS036705 from MyBiosource.com (San Diego CA); and for C-reactive protein (CRP) catalog #RAB0097 from Sigma-Aldrich Inc. (St. Louis, MO). All serum assays were performed in triplicate.
Frohbergh M., Ge Y., Meng F., Karabul N., Solyom A., Lai A., Iatridis J., Schuchman E.H, & Simonaro C.M. (2014). Dose Responsive Effects of Subcutaneous Pentosan Polysulfate Injection in Mucopolysaccharidosis Type VI Rats and Comparison to Oral Treatment. PLoS ONE, 9(6), e100882.
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2

Oxidative Stress and Inflammatory Markers Assay

Cited 1 time
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DRG were dissociated with a mortar and pestle in tissue extraction reagent supplied with protease and phosphatase inhibitors as previously described.27 (link),28 (link) Assay kits for glutathione (703002, Cayman Chemical, Ann Arbor, MI, USA) (detection range: 0.25–16 μM) superoxide dismutase (706002, Cayman Chemical) (detection range: 0.005–0.050 U/ml), DNA/RNA oxidative damage (589320, Cayman Chemical) (detection range: 10.3–3,000 pg/ml), IL-1β (RLB00, R&D Systems, Minneapolis, MN) (detection range: 5–2,000 pg/ml), TNF (RTA00, R&D Systems) (detection range: 5–800 pg/ml), and CCL2 (ab100778, Abcam) (detection range: 24.67–18,000 μM), were used according to manufacturer instructions. Results were normalized to total protein levels (Bradford protein assay).
Li J., Ma J., Lacagnina M.J., Lorca S., Odem M.A., Walters E.T., Kavelaars A, & Grace P.M. (2020). Oral dimethyl fumarate reduces peripheral neuropathic pain in rodents via NFE2L2 antioxidant signaling.. Anesthesiology, 132(2), 343-356.
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3

Serum Biomarker Quantification via ELISA

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We measured serum CGRP (CSB-E08211r, CUSABIO TECHNOLOGY LLC, Houston, Texas.), IL-1β (RLB00, R&D, Minnesota, USA), IL-6 (R6000B, R&D, Minnesota, USA), TNFα (RTA00, R&D), COX-2 (CSB-E13399r, CUSABIO TECHNOLOGY LLC), and BDNF (DBNT00, R&D) levels using ELISA according to the method recommended by the kit manufacturer.
Zhao L., Liu L., Xu X., Qu Z., Zhu Y., Li Z., Zhao J., Wang L., Jing X, & Li B. (2020). Electroacupuncture Inhibits Hyperalgesia by Alleviating Inflammatory Factors in a Rat Model of Migraine. Journal of Pain Research, 13, 75-86.
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4

Renal Cytokine Level Determination

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The renal levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were determined using ELISA kits purchased from Thermo Fisher Scientific (catalogue number: ERIL1B) and R&D Systems (catalogue number: RTA00), respectively. Analyses were performed according to the manufacturer's instructions.
Albasher G., Almeer R., Alarifi S., Alkhtani S., Farhood M., Al-Otibi F.O., Alkubaisi N, & Rizwana H. (2019). Nephroprotective Role of Beta vulgaris L. Root Extract against Chlorpyrifos-Induced Renal Injury in Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 3595761.
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5

Tissue Extraction and Cytokine Quantification

Cited 2 times
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Tissue samples were dissociated with a gentleMACS Octo Dissociator (Miltenyi Biotec, Auburn, CA) or a mortar and pestle in tissue extraction reagent (50 mM Tris buffer containing 100 mM 6-amino-n-caproic acid, 1 mM EDTA, 5 mM benzamidine, 0.2 mM phenylmethyl sulfonyl fluoride (in 100% ethanol)) supplied with protease and phosphatase inhibitors as previously described (Grace et al., 2016a (link), 2016c (link); Li et al., 2020 (link)). Assay kits for IL-1β (RLB00, R&D Systems, Minneapolis, MN) (detection range: 5 – 2,000 pg/mL), IL-6 (R6000B, R&D Systems) (detection range: 62.5 – 4,000 pg/mL), and TNF (RTA00, R&D Systems) (detection range: 5 – 800 pg/mL) were used according to manufacturer instructions. Results were normalized to total protein levels (Bradford protein assay).
Lacagnina M.J., Li J., Lorca S., Rice K.C., Sullivan K., O’Callaghan J.P, & Grace P.M. (2020). A role for neuroimmune signaling in a rat model of Gulf War Illness-related pain. Brain, behavior, and immunity, 91, 418-428.
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6

Serum Cytokine Profiling with ELISA

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Blood samples were collected into non-heparinized tubes and centrifuged at 3000 g for 10 min, and the obtained serum was stored at − 80 °C. The amount of Interleukin-6 (IL-6) (R6000B, R&D Systems), tumour necrosis factor-α (TNF-α) (RTA00, R&D Systems), and IL-1β (RLB00, R&D Systems) in serum samples were measured with an enzyme-linked immunosorbent assay (ELISA). In brief, known concentrations of recombinant rat IL-6, TNF-α, or IL-1β and the experimental samples were added and incubated in polystyrene microtiter plates coated with an antibody against the appointed cytokine, followed by incubation with an enzyme-linked polyclonal antibody directed to the cytokine. Next, a substrate solution for the enzyme was added, and the colour development was stopped by adding 2N H2SO4. The absorbance was measured with a microtiter plate spectrophotometer. The amount of IL-6, TNF-α, and IL-1β in each sample was determined from a standard curve generated in each assay and expressed as pictograms per millilitre.
Manshori M., Kazemnejad S., Naderi N., Darzi M., Aboutaleb N, & Golshahi H. (2022). Greater angiogenic and immunoregulatory potency of bFGF and 5-aza-2ʹ-deoxycytidine pre-treated menstrual blood stem cells in compare to bone marrow stem cells in rat model of myocardial infarction. BMC Cardiovascular Disorders, 22, 578.
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7

Quantification of Serum TNF-alpha

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Serum samples were analyzed using a commercial kit for TNF-α (#RTA00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer´s instructions. OD was analyzed at 450 nm on a microplate reader (Tecan®, Männedorf, Switzerland).
Scheid S., Goeller M., Baar W., Wollborn J., Buerkle H., Schlunck G., Lagrèze W., Goebel U, & Ulbrich F. (2021). Hydrogen Sulfide Reduces Ischemia and Reperfusion Injury in Neuronal Cells in a Dose- and Time-Dependent Manner. International Journal of Molecular Sciences, 22(18), 10099.
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8

Plasma TNF-α Quantification by ELISA

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Each plasma sample was analysed for TNF-α by ELISA using an ELISA kit (R&D Systems, RTA00, Minneapolis, MN, USA) according to the manufacturer's instructions.
Li J., Ke W., Zhou Q., Wu Y., Luo H., Zhou H., Yang B., Guo Y., Zheng Q, & Zhang Y. (2014). Tumour necrosis factor-α promotes liver ischaemia-reperfusion injury through the PGC-1α/Mfn2 pathway. Journal of Cellular and Molecular Medicine, 18(9), 1863-1873.
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9

Serum TNF-α Measurement Using ELISA

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The serum levels of TNF-α were determined using a commercial ELISA kit (cat. no. RTA00; R&D Systems, Inc., Minneapolis, MN, USA), according to the manufacturer's protocol. Differences in TNF-α levels were calculated by subtracting the serum levels of TNF-α at different time points (2, 4, and 8 weeks) from the serum levels prior to treatment.
Wang L., Tang X., Xiang X., Tang Y, & Qiu L. (2019). Experimental study of TNF-α receptor gene transfection by ultrasound-targeted microbubble destruction to treat collagen-induced arthritis in rats in vivo. Experimental and Therapeutic Medicine, 17(3), 1601-1610.
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10

Hippocampal Inflammatory Cytokine Quantification

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Hippocampal level of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured using enzyme-linked immunosorbent assay kits sourced from ThermoFisher Scientific (Catalogue number ERIL1B) and R&D Systems (Catalogue number RTA00) following the manufacturers’ protocol.
Yuan X., Fu Z., Ji P., Guo L., Al-Ghamdy A.O., Alkandiri A., Habotta O.A., Abdel Moneim A.E, & Kassab R.B. (2020). Selenium Nanoparticles Pre-Treatment Reverse Behavioral, Oxidative Damage, Neuronal Loss and Neurochemical Alterations in Pentylenetetrazole-Induced Epileptic Seizures in Mice. International Journal of Nanomedicine, 15, 6339-6353.
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