Log In Sign up
The largest database of trusted experimental protocols

Casp1 assay buffer

Manufactured by Enzo Life Sciences
Visit Supplier

CASP1 assay buffer is a solution used to maintain the activity and stability of the CASP1 enzyme during experimental procedures. It provides the necessary environmental conditions for the enzyme to function properly.

Automatically generated - may contain errors

Lab products found in correlation

Ac yvad cho, by Enzo Life Sciences (2 mentions) Chemidoc imager, by Bio-Rad (2 mentions) Pvdf membrane, by Thermo Fisher Scientific (2 mentions) Novex bis tris gel, by Thermo Fisher Scientific (2 mentions) Anti tubulin, by Santa Cruz Biotechnology (2 mentions) Anti bim, by Cell Signaling Technology (2 mentions) Anti hsp90, by Cell Signaling Technology (2 mentions) Quantity one software, by Bio-Rad (2 mentions)

4 protocols using casp1 assay buffer

1

Caspase-1 Enzymatic Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human CASP1 (100–200 U, where U = 1 pmol/min at 30°C, 200 µM YVAD-pNA) from a CASP1 assay kit for drug discovery (Enzo Life Sciences, catalog number BML-AK701-0001) was incubated at 30°C with wild-type or mutated glucocorticoid receptor in the presence (10 µM unless otherwise indicated) or absence of inhibitors, (Ac-YVAD-CHO, Enzo Life Sciences, catalog number BML-P403-9090), VX765, VRT-043198. Substrates for enzyme assays were prepared in CASP1 assay buffer (Enzo Life Sciences, catalog number KI-111) consisting of 50 mM HEPES, pH7.4, 100 mM NaCl, 0.1% CHAPS, 10 mM DTT, 1 mM EDTA and 10% glycerol.
Paugh S.W., Bonten E.J., Savic D., Ramsey L.B., Thierfelder W.E., Gurung P., Malireddi R.K., Actis M., Mayasundari A., Min J., Coss D.R., Laudermilk L.T., Panetta J.C., McCorkle J.R., Fan Y., Crews K.R., Stocco G., Wilkinson M.R., Ferreira A.M., Cheng C., Yang W., Karol S.E., Fernandez C.A., Diouf B., Smith C., Hicks J.K., Zanut A., Giordanengo A., Crona D., Bianchi J.J., Holmfeldt L., Mullighan C.G., den Boer M.L., Pieters R., Jeha S., Dunwell T.L., Latif F., Bhojwani D., Carroll W.L., Pui C.H., Myers R.M., Guy R.K., Kanneganti T.D., Relling M.V, & Evans W.E. (2015). NALP3 inflammasome up-regulation and CASP1 cleavage of the glucocorticoid receptor causes glucocorticoid resistance in leukemia cells. Nature genetics, 47(6), 607-614.
+ Open protocol
+ Expand
2

Caspase-1 Enzymatic Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human CASP1 (100–200 U, where U = 1 pmol/min at 30°C, 200 µM YVAD-pNA) from a CASP1 assay kit for drug discovery (Enzo Life Sciences, catalog number BML-AK701-0001) was incubated at 30°C with wild-type or mutated glucocorticoid receptor in the presence (10 µM unless otherwise indicated) or absence of inhibitors, (Ac-YVAD-CHO, Enzo Life Sciences, catalog number BML-P403-9090), VX765, VRT-043198. Substrates for enzyme assays were prepared in CASP1 assay buffer (Enzo Life Sciences, catalog number KI-111) consisting of 50 mM HEPES, pH7.4, 100 mM NaCl, 0.1% CHAPS, 10 mM DTT, 1 mM EDTA and 10% glycerol.
Paugh S.W., Bonten E.J., Savic D., Ramsey L.B., Thierfelder W.E., Gurung P., Malireddi R.K., Actis M., Mayasundari A., Min J., Coss D.R., Laudermilk L.T., Panetta J.C., McCorkle J.R., Fan Y., Crews K.R., Stocco G., Wilkinson M.R., Ferreira A.M., Cheng C., Yang W., Karol S.E., Fernandez C.A., Diouf B., Smith C., Hicks J.K., Zanut A., Giordanengo A., Crona D., Bianchi J.J., Holmfeldt L., Mullighan C.G., den Boer M.L., Pieters R., Jeha S., Dunwell T.L., Latif F., Bhojwani D., Carroll W.L., Pui C.H., Myers R.M., Guy R.K., Kanneganti T.D., Relling M.V, & Evans W.E. (2015). NALP3 inflammasome up-regulation and CASP1 cleavage of the glucocorticoid receptor causes glucocorticoid resistance in leukemia cells. Nature genetics, 47(6), 607-614.
+ Open protocol
+ Expand
3

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
In brief, cells were pelleted by centrifugation, washed once with PBS and lysed with RIPA buffer or CASP1 assay buffer (Enzo Life Sciences, catalog number KI-111), equal amounts of proteins (1–20 µg) separated by 4–12% Novex Bis-Tris gels (Life Technologies), and then transblotted to PVDF membranes (Life Technologies). Anti-glucocorticoid receptor (1:1000–1:10,000, BD catalog number 611227), anti-CASP1 p20 (Adipogen catalog number AG-20B-0048), anti-DDK tubulin (1:1000, Origene catalog number TA50011), anti-Bim (1:1000, Cell Signaling catalog number 2819) anti-Tubulin (1:1000, Santa Cruz, sc-8035), or anti-HSP90 (1:1000, Cell Signaling catalog number 4874) were used as primary antibodies followed by appropriate secondary HRP-conjugated IgG (1:1000, Dako) and immunocomplexes were visualized by chemiluminescence using a Chemidoc Imager (BioRad). Signal intensities of CASP1 and glucocorticoid receptor where quantified using Quantity One Software (BioRad) and normalized for the signal intensity of the loading control in the same lane.
Paugh S.W., Bonten E.J., Savic D., Ramsey L.B., Thierfelder W.E., Gurung P., Malireddi R.K., Actis M., Mayasundari A., Min J., Coss D.R., Laudermilk L.T., Panetta J.C., McCorkle J.R., Fan Y., Crews K.R., Stocco G., Wilkinson M.R., Ferreira A.M., Cheng C., Yang W., Karol S.E., Fernandez C.A., Diouf B., Smith C., Hicks J.K., Zanut A., Giordanengo A., Crona D., Bianchi J.J., Holmfeldt L., Mullighan C.G., den Boer M.L., Pieters R., Jeha S., Dunwell T.L., Latif F., Bhojwani D., Carroll W.L., Pui C.H., Myers R.M., Guy R.K., Kanneganti T.D., Relling M.V, & Evans W.E. (2015). NALP3 inflammasome up-regulation and CASP1 cleavage of the glucocorticoid receptor causes glucocorticoid resistance in leukemia cells. Nature genetics, 47(6), 607-614.
+ Open protocol
+ Expand
4

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
In brief, cells were pelleted by centrifugation, washed once with PBS and lysed with RIPA buffer or CASP1 assay buffer (Enzo Life Sciences, catalog number KI-111), equal amounts of proteins (1–20 µg) separated by 4–12% Novex Bis-Tris gels (Life Technologies), and then transblotted to PVDF membranes (Life Technologies). Anti-glucocorticoid receptor (1:1000–1:10,000, BD catalog number 611227), anti-CASP1 p20 (Adipogen catalog number AG-20B-0048), anti-DDK tubulin (1:1000, Origene catalog number TA50011), anti-Bim (1:1000, Cell Signaling catalog number 2819) anti-Tubulin (1:1000, Santa Cruz, sc-8035), or anti-HSP90 (1:1000, Cell Signaling catalog number 4874) were used as primary antibodies followed by appropriate secondary HRP-conjugated IgG (1:1000, Dako) and immunocomplexes were visualized by chemiluminescence using a Chemidoc Imager (BioRad). Signal intensities of CASP1 and glucocorticoid receptor where quantified using Quantity One Software (BioRad) and normalized for the signal intensity of the loading control in the same lane.
Paugh S.W., Bonten E.J., Savic D., Ramsey L.B., Thierfelder W.E., Gurung P., Malireddi R.K., Actis M., Mayasundari A., Min J., Coss D.R., Laudermilk L.T., Panetta J.C., McCorkle J.R., Fan Y., Crews K.R., Stocco G., Wilkinson M.R., Ferreira A.M., Cheng C., Yang W., Karol S.E., Fernandez C.A., Diouf B., Smith C., Hicks J.K., Zanut A., Giordanengo A., Crona D., Bianchi J.J., Holmfeldt L., Mullighan C.G., den Boer M.L., Pieters R., Jeha S., Dunwell T.L., Latif F., Bhojwani D., Carroll W.L., Pui C.H., Myers R.M., Guy R.K., Kanneganti T.D., Relling M.V, & Evans W.E. (2015). NALP3 inflammasome up-regulation and CASP1 cleavage of the glucocorticoid receptor causes glucocorticoid resistance in leukemia cells. Nature genetics, 47(6), 607-614.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!