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Infinity analyze

Manufactured by Teledyne
Sourced in Canada

The Infinity Analyze is a versatile laboratory equipment designed for high-performance analysis and measurement. It offers a precise and reliable platform for a wide range of analytical applications.

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7 protocols using infinity analyze

1

Histological Analysis of Skin Samples

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The dorsal skin samples were collected in formol 10%, fixed in paraformaldehyde 4%, dehydrated in ascending concentrations of ethanol, cleared in xylene, embedded in paraffin and sectioned to a thickness of 5 μm. The sections were stained with hematoxylin and eosin, toluidine blue and Masson's trichrome stain.
The sections stained with H & E were examined using light microscopy at 40x magnification for determination of epidermal thickness (Deng et al., 2015 (link)) and a 100x magnification for counting the number of sunburn cells (Schwarz et al., 1995 (link)). For mast cell count, the sections were stained with toluidine blue and analyzed under light microscopy at 40x magnification. Both analyses were done with the software Infinity Analyze (Lumenera® Software). The sections stained with masson's trichrome were examined using light microscopy at a magnification of 10x to visualize changes in collagen fibers by analyzing the intensity of the blue coloration in the dermal areas of the skin exposed to UVB with the aid of the Image J software (NIH) (Song et al., 2016 (link)).
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2

Maize Kernel Tissue Preparation and Microscopic Analysis

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Six kernels from each ear replicate were fixed and dehydrated using the protocol modified from Livingston et al. (2009 (link), 2013 (link)) and Shu et al. (2015) (link). Briefly, we used a modified FAA fixative consisting of 45% methanol, 10% formaldehyde and 5% glacial acetic acid. The paraffin blocks were sectioned with a RM2255 microtome (Leica) and mounted on slides (Gold Seal). Slides were dried on a hot plate overnight and stored at room temperature. Paraffin was removed by dipping the slides in 100% xylene. Sections were then rehydrated with an ethanol series. Safranin and fast green staining were applied to differentiate tissue structure of maize kernels and the fungus grown in the kernel (Figures 1ch). The rehydrated sections were stained with safranin, dehydrated with an ethanol series, and counter stained with fast green (Fisher) (Shu et al., 2015 (link)). Stained sections were mounted in permount mounting medium (Fisher) and covered with coverslips. Images of stained tissues were collected on an Eclipse E600 light microscope (Nikon). Images were captured on an Infinity1-3C digital camera, and analyzed with the software Infinity Analyze (Lumenera).
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3

Microscopic Analysis of Fungal Structures

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Conidiomata and ascomata from fresh and dried specimens were mounted in deionized water, 5 % KOH, or lactic acid. Observations were made using an Olympus BX50 light microscope (Olympus, Tokyo) and an Olympus SZX12 stereomicroscope and micrographs were captured using an InfinityX-32 camera (Lumenera, Ottawa) and Infinity Analyze (Lumenera) software. Photographic plates were assembled using Adobe Photoshop 5.5 (Adobe Systems, San Jose, CA).
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4

Starch Granule Morphology Characterization

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Scanning electron micrographs of isolated starch granules were taken using a scanning electron microscope (JEOL 5800, Tokyo, Japan) following the methods previously reported (Ao & Jane, 2007) . The average starch granule size was determined by measuring ∼200 granules using an Infinity Analyze software (version 6.1.0, Lumenera Corp., Canada).
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5

Histological Evaluation of Bone Grafts

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The biopsies were fixed in 10% buffered formalin for 24 hours and decalcified in 20% formic acid for 3 hours. They were then processed and embedded in paraffin. The blocks were cut, and 5-μm-thick pieces were mounted on slides and stained with hematoxylin-eosin (Fig. 4, A andB). Images of the histological slides were captured by a digital camera (Infinity 1; Lumenera Corp., ON, Canada) coupled to an optical microscope (Nikon Eclipse Ci, Tokyo, Japan) under ×100 magnification and transferred to a computer running Infinity Analyze software (Lumenera). Histomorphometry was performed using Image J v. 1.47 software (National Institute of Health, Bethesda, MD), which allowed quantification of the percentages corresponding to MT and NMT. F4-20 Fig. 4: Histological sections of bone grafts harvested from the long axis in the control (A) and test (B) groups, stained with hematoxylin-eosin (×40 magnification).
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6

Enumeration of Cyanobacteria Cells

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A total of 1 mL of water samples that were previously preserved with Lugol’s solution were pipetted by using a Pasteur pipette into Sedgewick-Rafter counting chambers exactly filling it while avoiding the formation of air bubbles. The water sample was then given about 5–10 min to settle down before being counted grid by grid using a compound microscope starting from left to right [27 (link)]. This process was repeated 3 times for each sample to gather the average count of the result. The number of cells per filament of the cyanobacteria was obtained by multiplying the total filaments counted with the average number of cells of the first 30 filaments [28 ]. The morphological identification of cyanobacteria was done by using an inverted microscope: Olympus FluoView 300 (Olympus Corporation, Japan). The microscope incorporates the differential interference contrast (DIC) filter in the process that can produce high-quality, pseudo-3D images of cyanobacteria (Scientifica, n.d.). The camera used to capture the images was called Infinity 3 (Teledyne Lumenera, Ottawa, Canada), while the software used to analyze the image was Infinity Analyze (Teledyne Lumenera, Ottawa, Canada).
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7

Myelin Morphology in Cingulum and Corpus Callosum

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In the cingulum bundle (CB) and corpus callosum (CC), morphological alterations in myelin were observed and quantified. We obtained a total of 50 microscopic fields using a 40 × objective. Each area per field was previously calibrated at 19 700 μm2. Images were acquired using a CX31 Olympus microscope equipped with a digital camera Infinity1 (Teledyne Lumenera®), and the Infinity Analyze® software (version 6.3.0).
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