The sections stained with H & E were examined using light microscopy at 40x magnification for determination of epidermal thickness (Deng et al., 2015 (link)) and a 100x magnification for counting the number of sunburn cells (Schwarz et al., 1995 (link)). For mast cell count, the sections were stained with toluidine blue and analyzed under light microscopy at 40x magnification. Both analyses were done with the software Infinity Analyze (Lumenera® Software). The sections stained with masson's trichrome were examined using light microscopy at a magnification of 10x to visualize changes in collagen fibers by analyzing the intensity of the blue coloration in the dermal areas of the skin exposed to UVB with the aid of the Image J software (NIH) (Song et al., 2016 (link)).
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Histological Analysis of Skin Samples
The sections stained with H & E were examined using light microscopy at 40x magnification for determination of epidermal thickness (Deng et al., 2015 (link)) and a 100x magnification for counting the number of sunburn cells (Schwarz et al., 1995 (link)). For mast cell count, the sections were stained with toluidine blue and analyzed under light microscopy at 40x magnification. Both analyses were done with the software Infinity Analyze (Lumenera® Software). The sections stained with masson's trichrome were examined using light microscopy at a magnification of 10x to visualize changes in collagen fibers by analyzing the intensity of the blue coloration in the dermal areas of the skin exposed to UVB with the aid of the Image J software (NIH) (Song et al., 2016 (link)).
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Histological Evaluation of Bone Grafts
Enumeration of Cyanobacteria Cells
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