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Anti cep164

Manufactured by Santa Cruz Biotechnology
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Anti-CEP164 is a product offered by Santa Cruz Biotechnology. It is a laboratory reagent used for research purposes. This product functions as an antibody that specifically binds to the CEP164 protein, which is involved in the formation and maintenance of the primary cilium, a specialized cellular structure. The core function of Anti-CEP164 is to facilitate the detection and study of the CEP164 protein in biological samples.

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Lab products found in correlation

Anti α tubulin, by Merck Group (2 mentions) Anti flag, by Merck Group (1 mentions) Anti γ tubulin, by Merck Group (1 mentions) Anti uaf1, by Santa Cruz Biotechnology (1 mentions) Anti centrin2, by Santa Cruz Biotechnology (1 mentions) Anti rfc5, by Santa Cruz Biotechnology (1 mentions) Anti nuf2, by Abcam (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Cytochalasin d, by Merck Group (1 mentions) Ab65837, by Abcam (1 mentions) Anti actin a5441, by Merck Group (1 mentions) Anti pericentrin ab4448, by Abcam (1 mentions) Anti α tubulin t6199, by Merck Group (1 mentions) Alexa fluor 555 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 donkey anti goat igg, by Thermo Fisher Scientific (1 mentions) Anti centrin, by Merck Group (1 mentions) Anti γ tubulin, by Abcam (1 mentions)

Close spelling variants of this product

Cep164 (4 citations) Goat anti-CEP164 (2 citations)

3 protocols using anti cep164

1

Antibody Purification Procedure for ATAD5

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The following drug was used in this study: CDK1 inhibitor (RO3306; Sigma-Aldrich). The following antibodies were used: anti-UAF1, anti-Centrin2, anti-RFC5, anti-CEP164 (Santa Cruz Biotechnology); anti-FLAG, anti-HA, anti-γ-tubulin, anti-α-tubulin (Sigma-Aldrich); anti-NUF2 (Abcam); anti-histone H3 antibodies (Thermo Fisher Scientific). Polyclonal anti–human ATAD5 antibody was raised against the N-terminal fragment (residues 202–367 amino acids) in rabbits. The anti-ATAD5 antibody was purified using ATAD5 fragment (residues 1–400 amino acids) blotted to nitrocellulose membrane. Briefly, the blot was incubated with 5 mL of serum, washed three times with Tris-buffered saline containing 0.1% Tween 20. Then, the bound antibody was eluted with 0.1 M glycine buffer (pH 2.7) three times, and the eluted antibody was neutralized with 1/10 volume of 1.5 M Tris (pH 8.8).
Kim S.J., Wie M., Park S.H., Kim T.M., Park J.H., Kim S., Myung K, & Lee K.Y. (2020). ATAD5 suppresses centrosome over-duplication by regulating UAF1 and ID1. Cell Cycle, 19(15), 1952-1968.
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2

Antibody Validation for Cell Biology Research

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The anti‐progerin (ab66587), anti‐lamin A (ab8980), anti‐aquaporin 1 (ab65837), anti‐Arl3b (ab136648), and anti‐pericentrin (ab4448) antibodies were purchased from Abcam. The anti‐lamin A/C antibody (for immunoblotting, customized) was generated by GeneTex (Taiwan). The anti‐emerin (sc‐15378), anti‐Arp2 (sc‐15389), anti‐WASP (sc‐8353), and anti‐CEP164 (used in Fig 7A, sc‐515403) antibodies were purchased from Santa Cruz Biotechnology. The anti‐acetylated tubulin (T6793), anti‐γ‐tubulin (T5326), anti‐TTBK2 (HPA018113), anti‐actin (A5441), and anti‐α–tubulin (T6199) antibodies were purchased from Sigma‐Aldrich. The anti‐nesprin 2 (PA5‐51933) and anti‐Arp3 (PA5‐80307) antibodies were purchased from Thermo Fisher Scientific. The anti‐CEP164 antibody (used in Fig 8A, 4533.00.02) was purchased from SDIX. The anti‐myosin Va antibody (NBP1‐92156) was purchased from Novus. The anti‐CP110 antibody (12780‐1‐AP) was purchased from ProteinTech. The anti‐keratin 8 antibody (AB 531826) was purchased from DSHB. The anti‐centrin antibody (04‐1624) was purchased from Merck Millipore. Phalloidin (conjugated with Alexa Fluor‐488 or ‐546), DNase I (conjugated with Alexa Fluor‐488), and fetal bovine serum (FBS) were purchased from Invitrogen. Cytochalasin D was purchased from Sigma‐Aldrich. SAG was purchased from Enzo.
Fan J., You L., Wang W., Huang W., Chu C., Chi Y, & Chen H. (2020). Lamin A‐mediated nuclear lamina integrity is required for proper ciliogenesis. EMBO Reports, 21(10), e49680.
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3

Immunofluorescence of Centrosome Proteins

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Primary antibodies: anti-Centrin (Millipore, 04–1624), anti-γ-tubulin (Abcam, ab11321), anti-α-tubulin (Sigma, T5168), anti-Cep164 (Santa Cruz, sc-240226). Secondary antibodies for immunofluorescence microscopy: Donkey anti-Rabbit IgG Alexa Fluor 488 (Invitrogen, A21206), Donkey anti-Mouse IgG Alexa Fluor 555 (Invitrogen, A31570), Goat anti-Rat IgG Alexa Fluor 564 (Invitrogen, A11081), Donkey anti-Goat IgG Alexa Fluor 488 (Invitrogen, A21202).
Wang K., Muñoz K.J., Tan M, & Sütterlin C. (2021). Chlamydia and HPV induce centrosome amplification in the host cell through additive mechanisms. Cellular microbiology.
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