Urea

The activities of plasma enzymes alanine aminotransferase (ALT) (Abcam, Cambridge, MA, USA), aspartate aminotransferase (GOT) (Abcam, Cambridge, MA, USA), alkaline phosphatase (Abcam, Cambridge, MA, USA), in addition to the plasma levels of total bilirubin (Abcam, Cambridge, MA, USA), albumin (Abcam, Cambridge, MA, USA), chloride (Abcam, Cambridge, MA, USA), blood urea nitrogen (BUN) (Fisher Scientific, Madrid, Spain), creatinine (Abcam, Cambridge, MA, USA), calcium (Abcam, Cambridge, MA, USA), phosphate (Abcam, Cambridge, MA, USA), sodium (Abcam, Cambridge, MA, USA), potassium (Abcam, Cambridge, MA, USA), urea and uric acid (Abcam, Cambridge, MA, USA), were determined by commercial assay kits.
The determinations of CAT, SOD and GPx in the rabbit retinas were made using the Catalase Assay Kit, the Superoxide Dismutase Assay Kit and the Glutathione Peroxidase Assay Kit (Cayman Chemical, Ann Arbor, MI, USA) by spectrophotometry following the manufacturer protocols.

Sarpogrelate was obtained from Shanghai Linebon Ltd., Shanghai, China. Bromocriptine was sourced from Novartis, Italy. Cabergoline was purchased from Pfizer, Italy. Domperidone was purchased from Jamjoom Pharmaceuticals Co., KSA. Alloxan was obtained from Sigma-Aldrich (St Louis, MO, USA). Urea, creatinine, alkaline phosphatase, aspartate aminotransferase activity, LDH-1 assay kits, and the TNF alpha ELISA kit were obtained from Abcam, USA. The troponin I test kit was obtained from Encode Medical Engineering Company, Jeddah, KSA. 2,3,5-Triphenyltetrazolium chloride was obtained from Gold Biotechnology, USA.