Rt pcr synthesis kit

RNA was isolated from uninfected and infected dNK cells using TRIzol and chloroform and then precipitated using isopropanol. Generation of cDNA was accomplished using a Thermo Fisher High-Capacity cDNA Reverse Transcription Kit following the manufacturer’s instructions, and RT-PCR was catalyzed using an RT-PCR synthesis kit (Qiagen, Germany). The following primers were used: h2B4-A: F: 5′-CCTCTACTGCCTGGAGGTCACCAG-3′, R: 5′-CCACTTGGCATCTCCCTCTGTCC-3′; h2B4-B: F: 5′-GCCTTCCAATACTTCC-3′, R: 5’-TGGAAGCAGAGATTC-3′; SHP-2: F: 5′-AAAGGGGAGAGCAATGACGG-3′, R: 5′-GGGGCTGCTTGAGTTGTAGT-3′. Measurements were normalized to actin: F: 5′-GCACCGTCAAGGCTGAGAAC-3′, R: 5′-TGGTGAAGACGCCAGTGGA-3′.

The RNA of dNK cells or dMφ from the NOR and INF groups was isolated using Trizol and chloroform and then precipitated with isopropanol. cDNA was generated using a Thermo-Fisher High-Capacity cDNA Reverse Transcription Kit following the manufacturer’s instructions, and reverse transcriptase polymerase chain reaction (RT-PCR) was conducted using an RT-PCR synthesis kit (Qiagen, Germany) (Additional file 6: Table S1).