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Human il 1 beta il 1f2 duoset elisa kit

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The Human IL-1 beta/IL-1F2 DuoSet ELISA Kit is a tool for the quantitative determination of human interleukin-1 beta (IL-1 beta) and interleukin-1 family member 2 (IL-1F2) levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Mouse il 1β il 1f2 quantikine elisa kit, by R&D Systems (1 mentions) Mouse il 1 beta il 1f2 duoset elisa kit, by R&D Systems (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Infinite f200, by Tecan (1 mentions) Human tnf alpha quantikine elisa kit, by R&D Systems (1 mentions) Human il 8 cxcl8 quantikine elisa kit, by R&D Systems (1 mentions) Human il 6 quantikine elisa kit, by R&D Systems (1 mentions) Victor nivo hh3500, by PerkinElmer (1 mentions)

8 protocols using human il 1 beta il 1f2 duoset elisa kit

1

Quantifying IL-1β in Fungal Infection

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Mouse BMDMs and hMDMs were infected with C. albicans (MOI = 10) for the indicated time periods. IL-1β in culture supernatants was measured with a mouse IL-1β/IL-1F2 Quantikine ELISA Kit or human IL-1 beta/IL-1F2 DuoSet ELISA Kit (R&D Systems). To measure intracellular IL-1β, BMDMs were collected and lysed with PBS/0.5% Triton X-100. Proteins in the supernatants were precipitated with chloroform/methanol (1:4). Unattached cells (<5%) were removed by washing twice with PBS. The levels of IL-1β in each sample were measured using the ELISA kit as described above.
Ding X., Kambara H., Guo R., Kanneganti A., Acosta-Zaldívar M., Li J., Liu F., Bei T., Qi W., Xie X., Han W., Liu N., Zhang C., Zhang X., Yu H., Zhao L., Ma F., Köhler J.R, & Luo H.R. (2021). Inflammasome-mediated GSDMD activation facilitates escape of Candida albicans from macrophages. Nature Communications, 12, 6699.
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2

Cytokine Expression Analysis in Mice

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For investigation of the effect of FDP on the expression of cytokines, a subset of mice were sacrificed and brain regions and plasma were harvested. Brain regions were lysed with 1X Cell Lysis Buffer supplemented with 1 mM PMSF (Cell Signaling, Danvers MA) and Protease Inhibitor Cocktail (Sigma-Aldrich, cat no. 11873580001). Brain tissue IL-1β was measured with Mouse IL-1 beta/IL-1F2 DuoSet ELISA Kit (R&D Systems, Minneapolis MN) according to the manufacturer’s instructions. For mouse primary microglia and human THP-1 macrophages, tissue culture supernatant was harvested for analysis of secreted cytokines. In human THP-1 cells, IL-1β was measured with Human IL-1 beta/IL-1F2 DuoSet ELISA Kit (R&D Systems) according to the manufacturer’s instructions.
Smith C., Trageser K.J., Wu H., Herman F.J., Iqbal U.H., Sebastian-Valverde M., Frolinger T., Zeng E, & Pasinetti G.M. (2021). Anxiolytic effects of NLRP3 inflammasome inhibition in a model of chronic sleep deprivation. Translational Psychiatry, 11, 52.
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3

Quantifying IL-1β in Activated Microglia

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Human IL-1 beta/IL-1F2 Duoset ELISA kit (R&D Systems, Catalog # DY201 was used to measure IL-1β in the supernatants of activated microglia. The assay was carried out according to the manufacturer’s instructions.
Albornoz E.A., Amarilla A.A., Modhiran N., Parker S., Li X.X., Wijesundara D.K., Aguado J., Zamora A.P., McMillan C.L., Liang B., Peng N.Y., Sng J.D., Saima F.T., Fung J.N., Lee J.D., Paramitha D., Parry R., Avumegah M.S., Isaacs A., Lo M.W., Miranda-Chacon Z., Bradshaw D., Salinas-Rebolledo C., Rajapakse N.W., Wolvetang E.J., Munro T.P., Rojas-Fernandez A., Young P.R., Stacey K.J., Khromykh A.A., Chappell K.J., Watterson D, & Woodruff T.M. (2022). SARS-CoV-2 drives NLRP3 inflammasome activation in human microglia through spike protein. Molecular Psychiatry, 28(7), 2878-2893.
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4

Quantification of IL-1β Release

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After exposure, the medium was removed, briefly centrifuged, and the supernatant was frozen for IL-1β analysis. The viability of the cells was tested by using Alamar blue assay. IL-1β release was analyzed by using Human IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D systems), according to the instructions from the manufacturer. The light absorbance was measured at 540 nm in a microplate reader (Tecan, Infinite F 200, Austira GmbH, Software: Magellan 7.2), and a linear standard curve was generated and used to determine the IL-1β concentration.
Cappellini F., Di Bucchianico S., Karri V., Latvala S., Malmlöf M., Kippler M., Elihn K., Hedberg J., Odnevall Wallinder I., Gerde P, & Karlsson H.L. (2020). Dry Generation of CeO2 Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure. Nanomaterials, 10(4), 618.
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5

Cytokine Quantification in Cell Supernatant

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The supernatant was collected after the treatments, and the IL-1β, IL-6, IL-8, and TNF-α levels were analyzed using Human IL-1 beta/IL-1F2 DuoSet ELISA Kit (R&D systems, catalog number: DY201-05), Human IL-6 Quantikine ELISA Kit (R&D systems, catalog number: D6050), Human IL-8/CXCL8 Quantikine ELISA Kit (R&D systems, catalog number: D8000C), or Human TNF-alpha Quantikine ELISA Kit (R&D systems, catalog number: DTA00D), as directed by the manufacturer. Finally, the protein concentration was determined with a microplate reader (Thermo Fisher Scien- tific, UK).
Wang J., Li G., Lin M., Lin S, & Wu L. (2022). microRNA-338-3p suppresses lipopolysaccharide-induced inflammatory response in HK-2 cells. BMC Molecular and Cell Biology, 23, 60.
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6

Quantification of IL-1β in Osteoblasts and Fibroblasts

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Quantification of interleukin 1β of osteoblast and fibroblast cultures (n = 4) at 1 and 3 days were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA). The optical density was detected by multimode microplate reader (VICTOR NivoTM HH3500, PerkinElmer®, Beaconsfield, UK). The results were measured in units of absorbance (AU) relative to the light intensity values and were converted in pg/mL according to the standard curve.
Fernandes B.F., Silva N., Marques J.F., Da Cruz M.B., Tiainen L., Gasik M., Carvalho Ó., Silva F.S., Caramês J, & Mata A. (2023). Bio-Piezoelectric Ceramic Composites for Electroactive Implants—Biological Performance. Biomimetics, 8(4), 338.
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7

Quantification of IL-1β in HDFs

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Supernatants from HDFs’ cultures were harvested, and the levels of IL-1β were assessed using a Human IL-1 beta/IL-1F2 DuoSet ELISA Kit (DY201-05, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s protocols.
Yan C.Y., Zhu Q.Q., Guan C.X., Xiong G.L., Chen X.X., Gong H.B., Li J.W., Ouyang S.H., Kurihara H., Li Y.F, & He R.R. (2024). Antioxidant and Anti-Inflammatory Properties of Hydrolyzed Royal Jelly Peptide in Human Dermal Fibroblasts: Implications for Skin Health and Care Applications. Bioengineering, 11(5), 496.
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8

Measuring IL-1β Levels in Ozone-Exposed Tissues

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After collecting the tissues media at the two different time-points upon O3 exposure (0 and 24 h), IL-1β levels were measured by using the Human IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D System cat DY201, Minneapolis, MN, USA), according to the manufacturer’s protocol. IL-1β levels were adjusted for media (pg/mL) and expressed as arbitrary units (AU%). Gen5 software (BioTek, USA) was used for the detection [35 (link)].
Sguizzato M., Ferrara F., Mariani P., Pepe A., Cortesi R., Huang N., Simelière F., Boldrini P., Baldisserotto A., Valacchi G, & Esposito E. (2021). “Plurethosome” as Vesicular System for Cutaneous Administration of Mangiferin: Formulative Study and 3D Skin Tissue Evaluation. Pharmaceutics, 13(8), 1124.
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