Anti phospho rps6

Manufactured by Abcam

Anti-phospho-rpS6 is a lab equipment product that detects the phosphorylated form of the ribosomal protein S6 (rpS6). rpS6 is a component of the 40S ribosomal subunit and its phosphorylation is associated with increased protein synthesis.

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Lab products found in correlation

Anti smac, by Abcam (1 mentions) Total oxphos human wb antibody cocktail, by Abcam (1 mentions) Anti 4e bp1, by Cell Signaling Technology (1 mentions) Anti hsp70, by Abcam (1 mentions) Revert total protein stain, by LI COR (1 mentions) Anti vdac, by Santa Cruz Biotechnology (1 mentions) Anti phospho 4ebp1, by Cell Signaling Technology (1 mentions) Anti hsp90, by Enzo Life Sciences (1 mentions) Anti hsp60, by Abcam (1 mentions) Anti mia40, by Santa Cruz Biotechnology (1 mentions) Anti rps6, by Abcam (1 mentions) Anti ubiquitin, by Thermo Fisher Scientific (1 mentions) Rapamycin, by Merck Group (1 mentions) Anti actin, by MP Biomedicals (1 mentions) Anti hnrnpd, by Merck Group (1 mentions) Anti fmrp, by Cell Signaling Technology (1 mentions) Anti glua1, by Abcam (1 mentions) Anti 14 3 3ζ, by Santa Cruz Biotechnology (1 mentions) Anti map2, by Santa Cruz Biotechnology (1 mentions) Anti hnrnpa2b1, by Abcam (1 mentions)

Close spelling variants of this product

Anti-RPS6 (6 citations)

2 protocols using anti phospho rps6

Western Blot Antibodies for OXPHOS

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Antibodies used for Western blot included the Total OXPHOS Human WB Antibody Cocktail (#ab1104a1; Abcam), anti-Aco2 (#ab110321; Abcam), anti-4E-BP1 (#9644; Cell Signaling) and anti-phospho-4E-BP1 (#2855; Cell Signaling), anti-EF-1α1/2 (#sc-377439; Santa Cruz), anti-Hsp60 (#ab59457; Abcam), anti-Hsp70 (#ab47455; Abcam), anti-Hsp90 (#16F1; Enzo), anti-Mdh2 (#sc-293474; Santa Cruz), anti-Mia40 (#sc-365137; Santa Cruz), anti-rpS6 (#ab40820, Abcam); anti-phospho-rpS6 (#ab65748; Abcam), anti-SMAC (#ab8114; Abcam), anti-Tim22 (#14927-1-AP; Proteintech), anti-ubiquitin (#701339; Thermo Scientific), and anti-VDAC (#sc-390996; Santa Cruz). Total proteins were stained with REVERT Total Protein Stain (#926-11011; LI-COR).

Liu Y., Wang X., Coyne L.P., Yang Y., Qi Y., Middleton F.A, & Chen X.J. (2019). Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol. Molecular Biology of the Cell, 30(11), 1272-1284.

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Investigating Synaptic Protein Regulation

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Antibodies used in this study are as follows: anti-GluA1, anti–hnRNP A2/B1, anti–PSD-95 (Abcam), anti–phospho-RPS6, anti–phospho-ERK (extracellular signal–regulated kinase), anti-FMRP (Cell Signaling), anti–N-term-GluA1, anti–N-term-GluA2, anti–hnRNP D (Millipore), anti-MAP2, anti–hnRNP A1, anti–14-3-3ζ (Santa Cruz), anti–hnRNP Q, anti-Flag (Sigma-Aldrich), anti-NMDAR1 (Synaptic Systems), and anti-actin (MPBIO). To inhibit translation, SHSY5Y cells were treated with 10 nM RAD001 or cycloheximide (100 μg/ml) and harvested at the indicated times. To block cap-dependent translation or induce synaptic stimulation, neurons were treated with 20 nM rapamycin (Sigma-Aldrich) or recombinant BDNF (100 ng/ml) (PeproTech), respectively.

Jung Y., Seo J.Y., Ryu H.G., Kim D.Y., Lee K.H, & Kim K.T. (2020). BDNF-induced local translation of GluA1 is regulated by HNRNP A2/B1. Science Advances, 6(47), eabd2163.

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