Osteogenic cells were fixed with an acetone/citrate buffer pH 4.2 (1.5:1) for 5 min at room temperature. Cells were stained with Napthol-AS-TR-phosphate solution (Sigma-Aldrich, Hamburg, Germany) for 1 h at room temperature. The staining solution consists of 1:1 v/v Napthol-AS-TR-phosphate solution (Napthol-AS-TR-phosphate diluted 1:5 in H2O) and Fast Red TR solution (Sigma-Aldrich ApS, Hamburg, Germany) (diluted 1:1.2 in 0.1 M Tris buffer, pH 9.0).
Fast red tr solution
Manufactured by Merck Group
Sourced in Germany
Fast Red TR solution is a laboratory reagent used in various analytical and diagnostic applications. It is a diazonium salt that can be used for the detection and visualization of specific biomolecules, such as enzymes, in biological samples. The solution provides a bright red color when it reacts with the target analyte, enabling its identification and quantification. The core function of Fast Red TR solution is to serve as a chromogenic substrate for the detection and localization of target molecules in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fast red tr solution
1
Osteogenic Cell Staining Protocol
2
Osteoblast Differentiation Histochemistry
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At 7 and 14 days during osteoblast differentiation, cells were fixed with acetone/citrate (10 mM) buffer, pH 4.2 (1.5:1), for 5 minutes at room temperature and stained with the 1:1 mixture of 0.2 mg/ml Napthol-AS-TR-phosphate (Sigma) and 1.3 mg/ml Fast Red TR solution (Sigma) in 0.1 M Tris buffer, pH 9.0, for 1 hour at room temperature.
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