Part of each biopsy was fixed in phosphate-buffered formalin, processed (Leica TP 1020, Leica Microsystems), embedded in paraffin, and cut into 5-μm-thick sections. The sections were subjected to high-temperature antigen retrieval for 10 min in EDTA buffer, pH 8, followed by blocking and incubation with antibodies against macrophages (Monoclonal mouse anti-human CD68, clone PG-M1, M0876, Dako, Glostrup, Denmark), mast cells (monoclonal mouse anti-human mast cell tryptase, clone AA1, M7052, Dako), and von Willebrand factor (polyclonal rabbit anti-human von Willebrand Factor, A0082, Dako) to detect vessels. For visualization of immunoreactions, MACH3 mouse AP-polymer (M3M532) was used for macrophages and mast cells and MACH3 Rabbit AP-Polymer (M3R533) for vessels (Biocare Medical, Concord, CA). The chromogen was Warp Red (WR806, Biocare Medical). Slides were counterstained with hematoxylin, mounted, scanned with a Mirax Digital Desk Scanner (Zeiss, Göttingen, Germany), and analyzed with Tissue Studio 3.6.1 (Definiens, Munich, Germany). Macrophage and mast cell densities were expressed as the number of immunoreactive cells per 103 adipocytes. Vessel density was expressed as positive signal area per adipocyte.
Mirax digital desk scanner
Manufactured by Zeiss
Sourced in Germany
The Mirax Digital Desk Scanner is a high-performance scanning device designed for professional use. It offers fast and reliable digital imaging capabilities for a variety of lab applications. The scanner captures detailed images at high resolutions, providing users with accurate digital representations of physical samples or documents. Its core function is to digitize and convert analog content into a digital format for further processing or analysis.
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Lab products found in correlation
Tp1020, by Leica (2 mentions)
Pg m1, by Agilent Technologies (2 mentions)
Tissue studio 3, by Definiens (2 mentions)
Warp red, by Biocare Medical (2 mentions)
A0082, by Agilent Technologies (1 mentions)
M7052, by Agilent Technologies (1 mentions)
M0876, by Agilent Technologies (1 mentions)
Mach 3 mouse ap polymer, by Biocare Medical (1 mentions)
2 protocols using mirax digital desk scanner
1
Quantitative Immunohistochemistry of Macrophages, Mast Cells, and Vessels in Adipose Tissue
2
Quantitative Histological Analysis of Adipose Tissue
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Part of each biopsy was fixed in phosphate-buffered formalin, processed (Leica TP 1020, Leica Microsystems), embedded in paraffin and cut into 5-μm-thick sections. The sections were subjected to high-temperature antigen retrieval for 10 min in EDTA buffer, pH 8, followed by blocking and incubation with antibodies against macrophages (monoclonal mouse anti-human CD68 clone PG-M1, Dako, Glostrup, Denmark), mast cells (monoclonal mouse anti-human mast cell tryptase, clone AA1, Dako), and von Willebrand factor (polyclonal rabbit anti-human von Willebrand factor, Dako) to detect vessels. For visualization of immunoreactions, MACH3 mouse AP-polymer was used for macrophages and mast cells and MACH3 rabbit AP-polymer for vessels (Biocare Medical, Concord, CA, USA). The chromogen was Warp Red (Biocare Medical). Slides were counterstained with hematoxylin, mounted, scanned with Mirax Digital Desk Scanner (Zeiss, Göttingen, Germany), and analyzed with Tissue Studio 3.6.1 (Definiens, Munich, Germany). Macrophage and mast cell densities were expressed as the number of immunoreactive cells per mm2 and per 103 adipocytes. Vessel density was expressed as the ratio of the positive signal area to the total specimen area.
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