The samples were preserved in 4% formaldehyde (made from paraformaldehyde) buffered in 0.1 M phosphate buffered saline (PBS), pH 7.2 [24 ] for 48 h, dehydrated in ethanol, and embedded in paraffin. Sections 4-6 μm thick were obtained in a Leica RM2255 microtome with the use of disposable steel blades and were stained with hematoxylin and eosin (HE). Micrographs were taken with an Olympus BX51 light microscope equipped with a digital camera and Image-Pro Express software (Media Cybernetics).
Bx51 light microscope
Manufactured by Media Cybernetics
The BX51 is a light microscope designed for a variety of scientific applications. It features a sturdy, ergonomic design and provides high-quality optical performance. The microscope is equipped with a range of objective lenses to accommodate different magnification requirements.
Automatically generated - may contain errors
Lab products found in correlation
Rm2255 microtome, by Leica (1 mentions)
Image pro express software, by Media Cybernetics (1 mentions)
Image pro software, by Media Cybernetics (1 mentions)
8 hydroxyquinoline, by Merck Group (1 mentions)
Onozuka r 10, by Serva Electrophoresis (1 mentions)
Image pro plus, by Media Cybernetics (1 mentions)
Abc elite kit, by Vector Laboratories (1 mentions)
4 protocols using bx51 light microscope
1
Tissue Preservation and Histological Analysis
2
Immunohistochemical Localization of Biglycan and Fibromodulin in Cementum and Dentin
3
Chromosome analysis of root meristems
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Root meristems representative of each phenotype (Menini Neto et al., 2007) , totaling 20 individuals were treated with 0.002M 8-hydroxyquinoline (Sigma ® , USA) at 9 o C for 24h, xed in Carnoy solution (ethanol: acetic acid, 3:1) and stored in a refrigerator, for at least 24h. The material was digested in enzymatic solution [2% (w/v) Onozuka R-10 (Serva ® )/20% (v/v) cellulase pectinase (Sigma ® )] at 37 o C for 7h. The slides were prepared according to Carvalho and Saraiva (1993) and stained with Giemsa 5% (Merck ® ). At least 20 cells of each individual were evaluated under an Olympus BX51 light microscope coupled to a scanning and image analysis system (Image Pro-Plus 4.5 Media Cybernetics ™ ). Chromosome length, short and long arms, ratio between chromosome arms, and the index of karyotype asymmetry were determined according to Watanabe et al., (1999) . The chromosomes were classi ed according to Levan et al., (1964) .
4
Liver and Mammary Gland Protein Localization
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Liver and MG paraffin sections (5 µm) were immunostained with Santa Cruz Biotechnology; rabbit polyclonal Δ6D (sc-98480) and Δ5D (sc-101953), and goat polyclonal ELOVL 5 (sc-374138) and ELOVL 2 (sc-54874) 1:200 dilution, ABC Elite kit, Vector Laboratories and visualised using 2•5 % nickel sulphate with 0•02 % DAB (3,3′-diaminobenzidine tetrahydrochloride) chromogen in 0•175 M-sodium acetate. Cell counts were performed on an Olympus BX51 light microscope using image analysis software (Image-Pro Plus, version 3.1; Media Cybernetics Inc.). For the data analyses of protein in the liver by IHC, we used the imageJ software as previously described (3) . For MG, the IHC was used only for immunolocalisation, as the distribution of lobules and fat was very different between control and MO mothers.
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