Rabbit anti grp78 bip pab

Antibodies (Abs) and their sources were as follows: rabbit anti-mouse NG2 polyclonal Ab (pAb) (5 μg/ml; Millipore, Billerica, MA), rat anti-mouse CD31 monoclonal Ab (mAb) (5 μg/ml; MEC13.3; BD Bioscience, San Jose, CA), goat anti-8-OHdG pAb (1/200; abcam, Cambridge, UK), rabbit anti-GRP78/BiP pAb (5 μg/ml; Proteintech, Chigcago, USA), rabbit anti-CHOP/GADD153 pAb (5 μg/ml; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Alexa 488- and Alexa 568-conjugated secondary Abs were obtained from Invitrogen (Carlsbad, CA).

Immunoblotting (IB) was performed as we previously described [25] (link), [26] (link), [27] (link), [28] (link). Briefly, the Blot Mini Gel Tank and Blot Module Set (Thermo) with precast polyacrylamide gels of NuPAGE 10% Bis−Tris protein gels (1.5 mm × 10 wells, Thermo) were used. Primary antibodies included rabbit anti-ISG15 mAb (Sinobiological, Beijing, China; 1:2000), rabbit anti-syntenin mAb (Abcam, Shanghai, China; 1:1000), rabbit anti-TSG101 mAb (Merck, Guangzhou, China; 1:2000), rabbit anti-CD9 pAb (Abcam; 1:2000), and rabbit anti-GRP78/BIP pAb (Proteintech, Wuhan, China; 1:2000). HRP-conjugated anti-rabbit IgG mAb (CST; 1:20000) was used as the secondary antibody.