The effect of DET on apoptosis was further verified by an annexin V-FITC and PI double staining kit (BD Biosciences, USA). Briefly, cells were incubated in medium containing DET with or without NAC. After 24 h, cells were collected with EDTA-free trypsin, rinsed with 4 °C precooled PBS before resuspension in 500 μL precooled binding buffer. Next, the cells were dyed with 5 μL of annexin V-FITC and 5 μL of PI. Finally, the samples were filtered by 300 aperture mesh filters, and apoptosis was detected by flow cytometry (BD Biosciences, San Jose, CA, USA).
Annexin 5 fitc and pi double staining kit
Manufactured by BD
Sourced in United States
The Annexin V-FITC and PI double staining kit is a laboratory reagent used for the detection and quantification of apoptotic cells. Annexin V-FITC binds to phosphatidylserine, which is exposed on the cell surface during apoptosis, while propidium iodide (PI) stains the DNA of cells with compromised cell membranes, indicating late apoptosis or necrosis. The kit provides a simple and effective method for the analysis of cell death processes.
Automatically generated - may contain errors
Lab products found in correlation
Flow cytometry, by BD (1 mentions)
Pvdf membrane, by Beyotime (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Phospho p38, by Abcam (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Caspase 3, by Abcam (1 mentions)
Phospho stat3, by Abcam (1 mentions)
Phospho jnk, by Abcam (1 mentions)
Enhanced chemiluminescence ecl detection kit, by Beyotime (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
2 protocols using annexin 5 fitc and pi double staining kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Recombinant CNTF Neuroprotection Assay
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Recombinant human CNTF was produced in Escherichia coli by our laboratory [37] . All cell culture reagents were purchased from Gibco (NY, USA). Aβ 1-42 , AG490, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (MO, USA). The Annexin V-FITC and PI double staining kit was obtained from BD Biosciences (CA, USA). Dimethyl sulfoxide (DMSO), RNase A, PVDF membranes and the enhanced chemiluminescence (ECL) detection kit were purchased from Beyotime (Nantong, China). Antibodies against Bcl-2, Bcl-xL and β-actin were obtained from Santa Cruz Biotechnology (CA, USA). Antibodies against JAK2, STAT3, phospho-JAK2, phospho-STAT3, JNK, ERK, p38, phospho-JNK, phospho-ERK and phospho-p38 were purchased from Abcam (MA, USA). Caspase-3 and caspase-9 fluorometric assay kits were obtained from BioVision (SF, USA). All other chemicals and reagents were of analytical grade.
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