Letrozole was kindly donated by Tofigh Daru (Tehran, Iran). Sorbitan monolaurate (Span20, Cat# S6635), Sorbitan monostearate (Span60, Cat# S7710), Sorbitan monooleate (Span80, Cat# S6760), Cholesterol (CHOL, Cat# C8667), dicetylphosphate (DCP, Cat# 850042P), Sodium dodecyl sulfate (SDS, Cat# L3771) and dialysis membrane (MWCO 12kDa, Cat# D6191), Nile red and Coumarin 6 were obtained from Sigma Aldrich (USA). Dimethyl sulfoxide (DMSO, Cat# 109678), chloroform (Cat# 107024), Amicon (Ultra-15-Membrane, MWCO 30kDa, Cat# C82301) were purchased from Merck (Germany). Trypsin-EDTA (Cat# 15400054), Trypan blue (Cat# 15250061), RPMI-1640 culture medium, DMEM (Dulbecco’s Modified Eagle culture medium, Cat# 11965092), phosphate-buffered saline (PBS, Cat# 10010049), fetal bovine serum (FBS, Cat# 16000044), MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Cat# M6494) and penicillin/streptomycin (PS, Cat# 10378016) 100 X were purchased from Gibco (USA). The MDA-MB-231, MCF-7, and MCF10A cell lines were obtained from Pasteur Cell Bank (Iran). The Annexin V-FITC flow cytometric kit was obtained from Affymetrix Biosciences (USA). RNA extraction and cDNA synthesis kits were purchased from Transgene Biotech, China (Cat#. ER101-01 and AE301-02).
Dialysis membrane mwco 12kda
Manufactured by Merck Group
Sourced in United States
The Dialysis membrane (MWCO 12kDa) is a semi-permeable membrane used in dialysis processes. It allows the passage of molecules with a molecular weight cut-off (MWCO) of 12 kilodaltons or less, while retaining larger molecules.
Automatically generated - may contain errors
Lab products found in correlation
Dicetyl phosphate, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
Sorbitan monostearate span 60, by Merck Group (1 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions)
Sorbitan monooleate span 80, by Merck Group (1 mentions)
Sorbitan monolaurate span 20, by Merck Group (1 mentions)
Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Nile red, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Coumarin 6, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
3 protocols using dialysis membrane mwco 12kda
1
Letrozole-Loaded Nanoparticle Formulations
2
In Vitro Drug Release from Silver Nanoparticles
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In vitro release of MP from the Ag NPs was performed using the dialysis bag method. A dialysis membrane (MWCO 12 kDa, Sigma-Aldrich, St. Louis, MO, USA) was hydrated in distilled water for 24 h. An accurately weighed amount of drug-loaded NPs (equivalent to the amount of MP in ratios of 1:1, 1:3, and 1:6) was dispersed in 10 mL of phosphate buffered saline (PBS) and then transferred to the dialysis bag, which was closed using a plastic clamp. Each bag was placed into a beaker containing 40 mL PBS (pH 7.4, dialysis medium). Aliquots (2 mL) from the dialysis medium were taken for measurements and subsequently replaced at predetermined time intervals with fresh medium. The drug-release study was performed for 24 h. The mean results of triplicate measurements and standard deviations were reported. The solution in the Falcon tube was shaken before each evaluation of UV–visible absorbance. For data analysis, the MP’s maximum absorption band values (λ = 192 nm) were employed. Additionally, drug-only controls (one drug control for each ratio) were made.
3
Capecitabine-β-CD Formulation Development
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β-CD (purity, >98%) was purchased from Sigma. The drug capecitabine (purity, >99%) was obtained from the Aria Pharmaceutical Company. Acrylic/maleic copolymer (purity, >92%) with a molar mass of 70 000 g mol−1 and brand CP 5 powder were purchased from BASF Germany. Dialysis membrane (MWCO 12 kDa) was purchased from Sigma. All other reagents and chemicals were of analytical grade and purchased from Sigma.
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