All reagents were used as received unless otherwise noted. Acrylic acid (monomer), 4,4′-azobis(4-cyanopentanoic acid) (radical initiator), and 4-cyano-4-(phenylcarbonothioylthio)pentanoic acid (chain transfer agent, CTA) were purchased from Sigma-Aldrich. D-Glucose, D-galactose, D-lactose monohydrate, D-maltose monohydrate, maltotriose, ammonium carbamate, ammonium hydroxide solution (28.0−30.0% NH3), and tris(2-carboxyethyl)phosphine hydrochloride were purchased from Sigma-Aldrich. 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) was purchased from TCI America. Octyl maleimide was purchased from Santa Cruz Biotechnology, Inc. HBS buffer (10 × 10−3 M 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES), 150 × 10−3 M NaCl, pH 7.2) was prepared with deionized (DI) water and filtered through a 0.2 μm PES membrane. Recombinant human galectin-1 and galectin-3 constructs were obtained from C. Bertozzi, recombinantly expressed in XL1-Blue competent E. coli, and purified using β-lactosyl sepharose affinity chromatography methods, as previously reported.42 (link)
Ammonium carbamate
Manufactured by Merck Group
Sourced in Germany
Ammonium carbamate is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water and other polar solvents. Ammonium carbamate is often used as a source of ammonia and carbon dioxide in various chemical reactions and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Carbamoyl phosphate disodium salt, by Merck Group (2 mentions)
Sodium cyanate, by Merck Group (2 mentions)
Trisodium trimetaphosphate, by Merck Group (2 mentions)
Ammonia 28 analar normapur, by Avantor (2 mentions)
Urea acs reagent, by Merck Group (2 mentions)
Deuterium oxide 99.90 d, by Eurisotop (2 mentions)
Ammonium carbonate acs reagent, by Merck Group (2 mentions)
Urease, by Merck Group (2 mentions)
Urea assay kit, by BioAssay Systems (2 mentions)
Maltotriose, by Merck Group (1 mentions)
4 cyano 4 phenylcarbonothioylthio pentanoic acid, by Merck Group (1 mentions)
D maltose monohydrate, by Merck Group (1 mentions)
D galactose, by Merck Group (1 mentions)
D lactose monohydrate, by Merck Group (1 mentions)
D glucose, by Merck Group (1 mentions)
Tris 2 carboxyethyl phosphine hydrochloride, by Merck Group (1 mentions)
4 4 6 dimethoxy 1 3 5 triazin 2 yl 4 methylmorpholinium chloride, by Tokyo Chemical Industry (1 mentions)
Biuret, by Merck Group (1 mentions)
L aspartic acid, by Merck Group (1 mentions)
Aerosil 380, by Evonik (1 mentions)
8 protocols using ammonium carbamate
1
Recombinant Galectin-1 and Galectin-3 Production
2
Carbamoyl Phosphate Synthesis Protocol
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The following chemical compounds were purchased from commercial suppliers and used without further purification: carbamoyl phosphate disodium salt (Sigma-Aldrich Co., cat. n°C4135-1G), ammonia 28% analaR Normapur (VWR Chemicals, cat n°21190.292), sodium cyanate (Sigma-Aldrich Co., cat. n°185086-100G), trisodium trimetaphosphate (Sigma-Aldrich Co., cat. n°T5508-500G), ammonium carbamate (Sigma-Aldrich Co., cat. n°292834-100G), urea ACS reagent (Sigma-Aldrich Co., cat. n°U5128-100G), ammonium carbonate ACS reagent (Aldrich chemical company, Inc., cat. n°20,786-1), deuterium oxide 99.90% D (Eurisotop, cat. n°D214FE).
3
Synthesis and Characterization of Ureidosuccinic Acid Derivatives
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The following chemical compounds were purchased from commercial suppliers and used without further purification: ureidosuccinic acid (Sigma-Aldrich Co., cat. n°69037-500MG), biuret (Sigma-Aldrich Co., cat. n°15270-25G), L-aspartic hemimagnesium salt dihydrate (Sigma-Aldrich Co., cat. n°11260-100G), L-aspartic acid (Sigma-Aldrich Co., cat. n°A8949-100G), magnesium carbonate basic (Sigma-Aldrich Co., cat. n°13118-1 KG) with a Brunauer–Emmett–Teller surface area of 31.5 m2/g, sodium cyanate (Sigma-Aldrich Co., cat. n°185086-100G), sodium hydroxide anhydrous pellets (Carlo Erba reagents, cat. n°480507), deuterium oxide 99.90% D (Eurisotop, cat. n°D214FE), linear dimer H-Asp-Asp-OH (Bachem, cat. n°4010210.0250), ammonium carbamate (Sigma-Aldrich Co., cat. n°292834-100G), ammonia 28% analaR Normapur (VWR Chemicals, cat n°21190.292), trisodium trimetaphosphate (Sigma-Aldrich Co., cat. n°T5508-500G), urea ACS reagent (Sigma-Aldrich Co., cat. n°U5128-100G), ammonium carbonate ACS reagent (Aldrich chemical company, Inc., cat. n°20786-1), carbamoyl phosphate disodium salt (Sigma-Aldrich Co., cat. n°C4135-1G), fumed silica Aerosil 380 (Evonik Industries), with a Brunauer–Emmett–Teller surface area of 380 m2/g.
4
Biocatalytic Reduction of Ketones
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Butyldiglycol (1a), nicotinamide adenine dinucleotide (NAD), reduced nicotinamide adenine dinucleotide (NADH), L-alanine, ammonium carbamate, and sodium carbonate were purchased from Sigma-Aldrich (Zwijndrecht, the Netherlands). Compounds 1b,c and 2a-c were prepared by BASF (Ludwigshafen, Germany; see Supporting Information). Substrate 2a was the (S)-enantiomer, in agreement with the enantioselectivity of the ADH that was selected. Pyridoxal phosphate (PLP) was purchased from Fisher Scientific (Landsmeer, the Netherlands) and pyruvic acid was acquired from Acros Organics (Geel, Belgium).
5
Urea Detection in Chronic Renal Failure
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Urease (from Canavalia ensiformis), urea, glutaraldehyde, disodium hydrogen phosphate, potassium dihydrogen phosphate, fluorescein, fluorescein isothiocyanate (FITC), and ammonium carbamate were obtained from Merck (Darmstadt, Germany). The urea assay kit was purchased from BioAssay Systems (Hayward, CA, USA). Phosphate buffered saline (PBS) was obtained from LPS solution (Daejeon, Korea) and used as the supporting electrolyte for the electrochemical measurements. Phosphate buffer (PB) was prepared by mixing 20 mM disodium hydrogen phosphate and 20 mM potassium dihydrogen phosphate (pH = 7.4), and it was used as the immobilization buffer. Porous polytetrafluoroethylene (PTFE) membranes with a pore size of 1 μm were purchased from Advantec MFS (Dublin, CA, USA). The waste peritoneal dialysate of a patient with chronic renal failure was obtained from the Seoul National University Hospital (Seoul, Korea) with written consent, and they agreed to participate according to the Declaration of Helsinki. The study was approved by the institutional review boards of Seoul National University Hospital (protocol number: SNUH IRB No. 1610-016-797).
6
Electrochemical Determination of Ammonium Carbamate
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All chemicals used for the study were of analytical reagent grades. Deionized water (Millipore Milli-Q System, Japan) was used to prepare the solutions used in this investigation. Sodium sulfite (Na2SO3), sulfuric acid (H2SO4), dipotassium hydrogen phosphate (K2HPO4), and potassium dihydrogen phosphate (KH2PO4) were purchased from Fujifilm Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Ammonium carbamate was purchased from Merck KGaA (Darmstadt, Germany). The glassy carbon (GC) electrode (diameter of 3 mm) was obtained from BAS Co., Ltd. (Tokyo, Japan).
7
Electrochemical Urea Biosensor Development
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urease, urea, glutaraldehyde, disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, and ammonium carbamate were purchased from Merck (Darmstadt, Germany). A urea assay kit was obtained from BioAssay Systems (Hayward, CA, USA). Phosphate-buffered saline (PBS) solution at a concentration of 20 mM was prepared by mixing 20 mM disodium hydrogen phosphate, 20 mM potassium dihydrogen phosphate, and 20 mM sodium chloride, and its pH was adjusted to 7.4. PBS was used as the supporting electrolyte during all the electrochemical measurements. All solutions were prepared using 18.2 MΩ purified water produced with a water purification system (Simplicity, Millipore, Burlington, MA, USA). All glassware and polyethylene materials were rinsed with ultrapure water and dried before use. Before each experiment, all stock solutions were freshly prepared.
8
Hydrogen Generation in Phosphate Buffer
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Ammonium carbamate was purchased from Merck (Germany) and used without further purification. Sulfuric acid (H2SO4) and sodium hydroxide (NaOH) were supplied by Wako Pure Chemical Industries, Ltd. (Japan). The test stock solution used was prepared by bubbling of the H2 gas into 0.10 M (M = mol dm -3 ) phosphate buffer (pH 7.0). H2 gas was continuously generated using a hydrogen generator (A9150-100, Parker, USA).
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