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Anti cd45 pe cf594 30 f11

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Anti-CD45-PE-CF594 (30-F11) is a fluorescent-labeled antibody used in flow cytometry applications. It targets the CD45 antigen, which is expressed on the surface of various leukocyte populations. The PE-CF594 fluorophore is conjugated to the antibody, enabling detection and analysis of CD45-positive cells.

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Lab products found in correlation

Anti cd4 pe, by Thermo Fisher Scientific (2 mentions) Rm4 4, by Thermo Fisher Scientific (2 mentions) Anti cd8β pe, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Anti-CD45 (213 citations) CD45-PE (130 citations) Anti-CD45-PE (55 citations) CD45 (30-F11, (38 citations) Anti-CD45 (30-F11, (27 citations) CD45 PE-CF594 (4 citations) Anti-CD45 PE-CF594 (3 citations)

2 protocols using anti cd45 pe cf594 30 f11

1

In vivo Immune Cell Labeling

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For in vivo labeling of circulating immune cells, anti-CD4-PE (eBioscience, RM4-4, 1:400) and anti-CD8β-PE (eBioscience, 1:400) were diluted in PBS and administered by IV injection 5 minutes before harvest(Anderson et al., 2014 (link)) Alternatively, anti-CD45-PE-CF594 (30-F11, BD Biosciences, 1:200) or anti-CD45-APC-ef780 (30-F11, eBioscience 1:40) were also used for intravascular labeling and were administered 2 or 5 minutes, respectively, before sacrifice. Mice were exposed ad libitum to drinking water containing dissolved FTY720 at a concentration of 2 μg/mL for 2 weeks starting at 6 weeks post-initiation.
Schenkel J.M., Herbst R.H., Canner D., Li A., Hillman M., Shanahan S.L., Gibbons G., Smith O.C., Kim J.Y., Westcott P.M., Hwang W.L., Freed-Pastor W.A., Eng G., Cuoco M.S., Rogers P., Park J.K., Burger M.L., Rozenblatt-Rosen O., Cong L., Pauken K.E., Regev A, & Jacks T. (2021). Conventional type I dendritic cells maintain a reservoir of proliferative tumor-antigen specific TCF-1+ CD8+ T cells in the tumor draining lymph nodes. Immunity, 54(10), 2338-2353.e6.
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2

In Vivo Immune Cell Labeling

Cited 1 time
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For in vivo labeling of circulating immune cells, anti-CD4-PE (eBioscience, RM4-4, 1:400) and anti-CD8β-PE (eBioscience, 1:400) were diluted in PBS and administered by IV injection 5 minutes before harvest (Anderson et al., 2012 (link)). Alternatively, anti-CD45-PE-CF594 (30-F11, BD Biosciences, 1:200) was also used for intravascular labeling and was administered 2 minutes before sacrifice.
Li A., Herbst R.H., Canner D., Schenkel J.M., Smith O.C., Kim J.Y., Hillman M., Bhutkar A., Cuoco M.S., Rappazzo C.G., Rogers P., Dang C., Jerby-Arnon L., Rozenblatt-Rosen O., Cong L., Birnbaum M., Regev A, & Jacks T. (2019). IL-33 Signaling Alters Regulatory T Cell Diversity in Support of Tumor Development. Cell reports, 29(10), 2998-3008.e8.
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