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Water immersion 40x 0.8 na objective

Manufactured by Olympus
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The Water immersion 40x/0.8 NA objective is a high-quality microscope objective lens designed for use in aqueous environments. It has a magnification of 40x and a numerical aperture (NA) of 0.8, which allows for high-resolution imaging. The lens is optimized for use with water-based samples and is suitable for a variety of microscopy applications.

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Lab products found in correlation

Bx50wi microscope, by Olympus (4 mentions) Borosilicate glass, by Sutter Instruments (4 mentions) Multiclamp 700b amplifier, by Molecular Devices (2 mentions)

Spelling variants of this product

60× water immersion objective (72 citations) 60×/1.2 NA water-immersion objective (12 citations)

4 protocols using water immersion 40x 0.8 na objective

1

Patch-clamp recording of neuronal slices

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neuronal slices were visualized using an Olympus BX50WI microscope equipped with oblique illumination and a water immersion 40x/0.8 NA objective (Olympus). Whole cell recordings (pipette resistance ~ 7 MΩ) were obtained using pipettes pulled from borosilicate glass (1.5mm and 1mm O.D, 0.86mm and 0.5mm I.D, Sutter Instruments Co., USA) and established using our custom-designed amplifier. The external bath comprised of artificial cerebral spinal fluid (ACSF) containing the following (in mM): 126 NaCl, 26 NaHCO3, 1.145 NaH2PO4, 10 glucose, 3 KCl, 2 MgSO4 and 2 CaCl2, Osmolarity ~300 mOsm. Patch pipettes were filled with internal solution containing (in mM): 130 K-gluconate, 5 NaCl, 2 MgSO4, 10 HEPES, 5 EGTA, 4 MgATP, 0.4 Na2GTP, 7 Na2-phospocreatine, 2 pyruvic acid, 0.002-0.01 alexa 488, pH adjusted to 7.2, ~280-290mOsm.
Shekar S., Jayant K., Rabadan M.A., Tomer R., Yuste R, & Shepard K.L. (2019). A miniaturized multi-clamp CMOS amplifier for intracellular neural recording. Nature electronics, 2(8), 343-350.
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2

Whole-cell patch-clamp recordings from interneurons

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Neuronal slices were visualized using an Olympus BX50WI microscope equipped with oblique illumination and a water immersion 40x/0.8 NA objective (Olympus). Labeled interneurons were first identified using epifluorescent illumination via a mercury lamp and appropriate emission filters prior to performing whole cell patch-clamp recordings. Whole cell recordings (pipette resistance 4–7MΩ) were obtained using pipettes pulled from borosilicate glass (1.5mm and 1mm O.D, 0.86mm and 0.5mm I.D, Sutter Instruments Co., USA) and established using a Multiclamp 700B amplifier (Molecular Devices, Union City, CA). The external bath comprised of artificial cerebral spinal fluid (ACSF) containing the following (in mM): 126 NaCl, 26 NaHCO3, 1.145 NaH2PO4, 10 glucose, 3 KCl, 2 MgSO4 and 2 CaCl2, Osmolarity ~300 mOsm. Patch pipettes were filled with internal solution containing (in mM): 130 K-gluconate, 5 NaCl, 2 MgSO4, 10 HEPES, 5 EGTA, 4 MgATP, 0.4 Na2GTP, 7 Na2-phospocreatine, 2 pyruvic acid, 0.002–0.01 alexa 488, pH adjusted to 7.2, ~280–290mOsm).
Jayant K., Wenzel M., Bando Y., Hamm J.P., Mandriota N., Rabinowitz J.H., Plante I.J., Owen J.S., Sahin O., Shepard K.L, & Yuste R. (2019). Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology In vivo. Cell reports, 26(1), 266-278.e5.
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3

Patch-clamp recording of neuronal slices

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neuronal slices were visualized using an Olympus BX50WI microscope equipped with oblique illumination and a water immersion 40x/0.8 NA objective (Olympus). Whole cell recordings (pipette resistance ~ 7 MΩ) were obtained using pipettes pulled from borosilicate glass (1.5mm and 1mm O.D, 0.86mm and 0.5mm I.D, Sutter Instruments Co., USA) and established using our custom-designed amplifier. The external bath comprised of artificial cerebral spinal fluid (ACSF) containing the following (in mM): 126 NaCl, 26 NaHCO3, 1.145 NaH2PO4, 10 glucose, 3 KCl, 2 MgSO4 and 2 CaCl2, Osmolarity ~300 mOsm. Patch pipettes were filled with internal solution containing (in mM): 130 K-gluconate, 5 NaCl, 2 MgSO4, 10 HEPES, 5 EGTA, 4 MgATP, 0.4 Na2GTP, 7 Na2-phospocreatine, 2 pyruvic acid, 0.002-0.01 alexa 488, pH adjusted to 7.2, ~280-290mOsm.
Shekar S., Jayant K., Rabadan M.A., Tomer R., Yuste R, & Shepard K.L. (2019). A miniaturized multi-clamp CMOS amplifier for intracellular neural recording. Nature electronics, 2(8), 343-350.
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+ Expand
4

Whole-cell patch-clamp recordings from interneurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neuronal slices were visualized using an Olympus BX50WI microscope equipped with oblique illumination and a water immersion 40x/0.8 NA objective (Olympus). Labeled interneurons were first identified using epifluorescent illumination via a mercury lamp and appropriate emission filters prior to performing whole cell patch-clamp recordings. Whole cell recordings (pipette resistance 4–7MΩ) were obtained using pipettes pulled from borosilicate glass (1.5mm and 1mm O.D, 0.86mm and 0.5mm I.D, Sutter Instruments Co., USA) and established using a Multiclamp 700B amplifier (Molecular Devices, Union City, CA). The external bath comprised of artificial cerebral spinal fluid (ACSF) containing the following (in mM): 126 NaCl, 26 NaHCO3, 1.145 NaH2PO4, 10 glucose, 3 KCl, 2 MgSO4 and 2 CaCl2, Osmolarity ~300 mOsm. Patch pipettes were filled with internal solution containing (in mM): 130 K-gluconate, 5 NaCl, 2 MgSO4, 10 HEPES, 5 EGTA, 4 MgATP, 0.4 Na2GTP, 7 Na2-phospocreatine, 2 pyruvic acid, 0.002–0.01 alexa 488, pH adjusted to 7.2, ~280–290mOsm).
Jayant K., Wenzel M., Bando Y., Hamm J.P., Mandriota N., Rabinowitz J.H., Plante I.J., Owen J.S., Sahin O., Shepard K.L, & Yuste R. (2019). Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology In vivo. Cell reports, 26(1), 266-278.e5.
+ Open protocol
+ Expand

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