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Cd4 qdot655 s3

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The CD4 Qdot655 (S3.5) is a fluorescently-labeled antibody used for the detection and analysis of CD4-positive cells in flow cytometry applications. It is designed to provide a specific and sensitive labeling of the CD4 cell surface marker.

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Lab products found in correlation

Cellsdirect 2x reaction mix, by Thermo Fisher Scientific (2 mentions) Cd3 alexa 700 sp34 2, by BD (2 mentions) Cd14 qdot605 tuk4, by Thermo Fisher Scientific (2 mentions) Hla dr pe cy7 g46 6, by BD (2 mentions) Cd45ra ecd 3p, by Beckman Coulter (2 mentions) Cd27 apc efluor780 o323, by Thermo Fisher Scientific (2 mentions) Cd8 pe sk1, by BD (2 mentions) Amine aqua amcyan, by Thermo Fisher Scientific (2 mentions) Rnaqueous micro lysis buffer, by Thermo Fisher Scientific (2 mentions) Cd16 fitc 3g8, by BD (2 mentions) Facsaria, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Cd3 apcefluor780 ucht1, by Thermo Fisher Scientific (2 mentions) Cd8 qdot 605 3b5, by Thermo Fisher Scientific (2 mentions) Hla dr pe l243, by BD (2 mentions)

Close spelling variants of this product

CD4–Qdot655

4 protocols using cd4 qdot655 s3

1

Multicolor Flow Cytometry Cell Sorting

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Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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2

Multiparameter Flow Cytometry of T Cell Subsets

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Methods have been previously reported [20 (link)]. Briefly, cryopreserved PBMCs were thawed and stained with surface antibodies against CD3-APCefluor780 (UCHT1; eBiosciences, San Diego, CA); CD4-Qdot 655 (S3.5) and CD8-Qdot 605 (3B5) (both from Life Technologies, Eugene, OR); CXCR3-APC-CD183 (1C6), CCR4 BV421-CD194 (1G1), CCR5-PE-Cy7-CD195 (2D7), CCR7-Alexa Fluor700-CD197 (150503), CD38-FITC (HB7) and HLA-DR-PE (L243) (all from BD Biosciences, San Jose, CA). Cells were then incubated for 30 minutes and centrifuged at 1200 rpm for 10 minutes. PBMCs were fixed with 2% formalin before analysis. Stained cells were acquired using an LSR II flow cytometer (BD Immunocytometry Systems, San Jose, CA). Using 10-color flow cytometric analysis, we investigated T cell phenotypes classified by the markers listed above. Data were analyzed using FlowJo software (v9.8.5, TreeStar, Ashland, OR). Lymphocytes were analyzed based on forward scatter (FSC) and side scatter (SSC) profiles. A single cell population was selected with SSC-A vs. SSC-H and then with FSC-A vs. FSC-H before gating for other markers. Gates were set based on Fluorescence Minus One (FMO) control.
Ogendi B.M., Bakshi R.K., Gupta K., Kapil R., Brown L.T., Jordan S.J., Sabbaj S., Press C.G., Lee J.Y, & Geisler W.M. (2017). T cell phenotypes in women with Chlamydia trachomatis infection and influence of treatment on phenotype distributions. Microbes and infection, 20(3), 176-184.
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3

Multiparameter Flow Cytometry for PBMC and MMC Characterization

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PBMCs and MMCs were stained with fluorescent antibodies against cell surface markers and analyzed using 10-color flow cytometry by previously reported methodology [3 (link)]. Briefly, PBMCs and MMCs were labeled with LIVE/DEAD fluorescent reactive dye (Invitrogen, Carlsbad, CA) and stained with surface antibodies against CD3-APCefluor780 (UCHT1; eBiosciences, San Diego, CA); CD4-Qdot 655 (S3.5) and CD8-Qdot 605 (3B5) (both from Life Technologies, Eugene, OR); CXCR3-APC-CD183 (1C6), CCR4 BV421-CD194 (1G1), CCR5-PE-Cy7-CD195 (2D7), CCR7-Alexa Fluor700-CD197 (150503), CD38-FITC (HB7) and HLA-DR-PE (L243) (all antibodies were purchased from BD Biosciences, San Jose, CA). Cells were then incubated for 30 minutes and centrifuged at 1200 rpm for 10 minutes. Both PBMCs and MMCs were fixed with 2% formalin before analysis. Using an LSR II flow cytometer (BD Immunocytometry Systems, San Jose, CA), CD3+ events were acquired from each sample. Data were analyzed using FlowJo software (v9.8.5, TreeStar, Ashland, OR). Lymphocytes analysis was based on forward and side scatter profiles after exclusion of dead cells (Fig 1). Gates were either set using negative sample or Fluorescence Minus One (FMO) control and were then applied to all samples from each participant.
Ogendi B.M., Bakshi R.K., Sabbaj S., Brown L., Lee J.Y., Kapil R, & Geisler W.M. (2017). Distinct peripheral versus mucosal T cell phenotypes in chlamydia-infected women. American journal of reproductive immunology (New York, N.Y. : 1989), 78(6), 10.1111/aji.12768.
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4

Multicolor Flow Cytometry Cell Sorting

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Mononuclear cell preparations were incubated in FACS staining buffer (PBS with 2% FBS and 2 mM EDTA) with fluorochrome-conjugated anti-human surface monoclonal antibodies (mAbs). Antibodies used included: CD3 Alexa-700 (SP34-2, BD Biosciences), CD14 Qdot605 (Tuk4, Invitrogen), CD16 FITC (3G8, BD PharMingen), HLA-DR PE-Cy7 (G46-6, BD Biosciences), CD4 Qdot655 (S3.5, Life Technologies), CD45RA ECD (3P, Beckman Coulter), CD27 APC-eFluor780 (O323, eBiosciences), CD25 PE(PC61, BD Biosciences), and CD8 PE(SK1, BD Biosciences). All cells were stained with a live/dead marker (Amine-Aqua/AmCyan; Invitrogen) to exclude dead cells from the analysis and sorting. Cells were then sorted by FACS (FACS Aria, BD Biosciences) into PBS. These cells were then re-sorted to a purity of greater than 99% directly into RNAqueous Micro lysis buffer (Ambion – Life Technologies). For Fluidigm qRT-PCR, cells were sorted into Cells Direct 2x Reaction Mix (Life Technologies).
Bunis D.G., Bronevetsky Y., Krow-Lucal E., Bhakta N.R., Kim C.C., Nerella S., Jones N., Mendoza V.F., Bryson Y.J., Gern J.E., Rutishauser R.L., Ye C.J., Sirota M., McCune J.M, & Burt T.D. (2021). Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells. Cell reports, 34(1), 108573.
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