Small intestines were cleaned and cut into duodenum, jejunum and ileum. Samples were fixed in 4% formaldehyde (Roth 4979.1), dehydrated and embedded in paraffin for sectioning. Sections were rehydrated and Alcian Blue staining was performed according to manufacturer’s instructions (Vector Laboratories H-3501).
H 3501
Manufactured by Vector Laboratories
The H-3501 is a high-performance laboratory centrifuge designed for efficient separation of various samples. It features a powerful motor and a robust construction to ensure reliable operation and consistent results.
Automatically generated - may contain errors
4 protocols using h 3501
1
Intestinal Tissue Alcian Blue Staining
2
Paraffin Tissue Histological Staining
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Paraffin sections (10μ) were dewaxed in Histo-Clear (2 × 10 minutes), then rehydrated through a decreasing ethanol gradient (2 × 100%, 1 × 90/70/50/30% in ddH2O, 2 minutes each) and washed in dH2O for 2 × 5 minutes. For H&E staining, slides were incubated in hematoxylin (Sigma, MHSS32) for 3 minutes and then rinsed in at least 3 changes of tap water for a total of ten minutes. Next, slides were incubated in eosin (Sigma, HT110232) for 90 s, washed in ddH2O for 2 minutes, and allowed to dry overnight. The next day, coverslips were mounted using DPX mounting media (Millipore, 100579).
Alcian blue staining was performed using an alcian blue staining kit (Vector, H-3501). Briefly, sections were incubated in alcian blue solution (pH2.5) for 30 minutes and counter stained with nuclear fast red, as per the manufacturer’s instructions. Sections were imaged using a Zeiss Axioplan2 Brightfield microscope with Zeiss Axiocam HRc color camera.
Alcian blue staining was performed using an alcian blue staining kit (Vector, H-3501). Briefly, sections were incubated in alcian blue solution (pH2.5) for 30 minutes and counter stained with nuclear fast red, as per the manufacturer’s instructions. Sections were imaged using a Zeiss Axioplan2 Brightfield microscope with Zeiss Axiocam HRc color camera.
3
Intestinal Tissue Alcian Blue Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Small intestines were cleaned and cut into duodenum, jejunum and ileum. Samples were fixed in 4% formaldehyde (Roth 4979.1), dehydrated and embedded in paraffin for sectioning. Sections were rehydrated and Alcian Blue staining was performed according to manufacturer’s instructions (Vector Laboratories H-3501).
4
Embryonic Lung Tissue Analysis
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