WT flies used were Canton S (CS). Lar and sns mutants have been previously described: Lar13.2, Lar451, and snsxb3 (gift of Dr. Susan Abmayr). The following lines were obtained from the Bloomington Stock Center: snsDf, C155-GAL4, tubulin-GAL4, OK107-GAL4, snsMiMIC MI03001, LarMiMIC02154, snsEY08142, UAS-Lar RNAi (TRiP.HMS02186), UAS-Lar RNAi (TRiP.HMS00822), and UAS-Kirre RNAi (TRiP.HMC05791). Sns RNAi lines were from the Vienna Drosophila Resource Center: UAS-Sns RNAi (KK109442) and UAS-Sns RNAi (GD877). T2A-GAL4 lines were generated as described in Diao et al., 2015 (link). Briefly, flies carrying the MiMIC insertion were crossed with flies bearing the triplet ‘Trojan exon’ donor. The F1 males from this cross carrying both genetic components were crossed to females carrying germline transgenic sources of Cre and ϕC31. The F2 males from this cross that had all four genetic components were then crossed to a UAS-2xEGFP reporter line, and the resulting progeny were screened for T2A-GAL4 transformants.
Uas lar rnai
Manufactured by Bloomington Drosophila Stock Center
The UAS-Lar RNAi is a genetic tool used for targeted gene knockdown in Drosophila. It contains a UAS (Upstream Activation Sequence) element that drives the expression of an RNA interference (RNAi) construct targeting the Lar (Leukocyte antigen-related) gene. This tool allows for the suppression of Lar gene expression in a spatially and temporally controlled manner when used in conjunction with a tissue-specific Gal4 driver line.
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Lab products found in correlation
Snsmimic mi03001, by Bloomington Drosophila Stock Center (2 mentions)
Larmimic02154, by Bloomington Drosophila Stock Center (2 mentions)
Uas sns rnai kk109442, by Vienna Drosophila Resource Center (2 mentions)
Tubulin gal4, by Bloomington Drosophila Stock Center (1 mentions)
C155 gal4, by Bloomington Drosophila Stock Center (1 mentions)
Ok107 gal4, by Bloomington Drosophila Stock Center (1 mentions)
2 protocols using uas lar rnai
1
Generating Drosophila Genetic Reporters
2
Generating T2A-GAL4 Fly Lines
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Wild-type (WT) flies used were Canton S (CS). Lar and sns mutants have been previously described: Lar 13.2 , Lar 451 and sns xb3 (gift of Dr. Susan Abmayr). The following lines were obtained from the Bloomington Stock Center: sns Df , C155-GAL4, tubulin-GAL4, sns MiMIC MI03001 , Lar MiMIC02154 , sns EY08142 , UAS-Lar RNAi (TRiP.HMS02186) and UAS-Lar RNAi (TRiP.HMS00822). Sns RNAi lines were from the Vienna Drosophila Resource Center: UAS-Sns RNAi (KK109442) and UAS-Sns RNAi (GD877). T2A-GAL4 lines were generated as described in (Diao et al., 2015) . Briefly, flies carrying the MiMIC insertion were crossed with flies bearing the triplet "Trojan exon" donor. The F1 males from this cross carrying both genetic components were crossed to females carrying germline transgenic sources of Cre and fC31. The F2 males from this cross that had all four genetic components were then crossed to a UAS-2xEGFP reporter line and the resulting progeny were screened for T2A-GAL4 transformants.
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