Su6656

Manufactured by MedChemExpress

SU6656 is a small molecule inhibitor that targets the SRC family kinases. It has an IC50 value in the nanomolar range for inhibiting the activity of SRC family kinases. SU6656 can be used for research purposes to study the biological functions of SRC family kinases.

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Lab products found in correlation

Protein a agarose, by Santa Cruz Biotechnology (1 mentions) Normal goat serum (ngs), by Jackson ImmunoResearch (1 mentions) Rpmi 1640 medium, by Sartorius (1 mentions) Mccoy s 5a modified medium, by Merck Group (1 mentions) Cell lighttm edu apollo 567 in vitro imaging kit, by RiboBio (1 mentions) Bovine serum albumin (bsa), by Roche (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Lactate assay kit, by Nanjing Jiancheng (1 mentions) Mg132, by MedChemExpress (1 mentions) Cycloheximide (chx), by MedChemExpress (1 mentions) Ly294002, by Cell Signaling Technology (1 mentions) Charcoal stripped serum, by Thermo Fisher Scientific (1 mentions) Palbociclib, by MedChemExpress (1 mentions) Fulvestrant, by Merck Group (1 mentions) 4 hydroxytamoxifen, by Merck Group (1 mentions) 17β estradiol e2, by Merck Group (1 mentions)

2 protocols using su6656

Optimized Cell Viability Assay

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Modified McCoy's 5A medium and MTT [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] reagent were from Sigma-Aldrich (St. Louis, MO). Trizol reagent and Lipofectamine 3000 were from Life Technology (Carlsbad, CA). Bovine serum albumin (BSA) was from Roche (Basel, Switzerland). Normal Goat Serum was from Jackson ImmunoResearch (West Grove, PA). RPMI 1640 medium and fetal bovine serum (FBS) were from Biological Industries (Kibbutz Beit Haemek, ISRAEL). High-sig ECL western blotting substrate was from Tanon (Shanghai, China). Protein A-Agarose was from Santa Cruz Biotechnology (Dallas, TX). Cell-Light^TM EdU Apollo^®567 In Vitro Imaging Kit was from RiboBio (Guangzhou, China). Lactate assay kit was from Jiancheng Biotech (Nanjing, China). MG132, cycloheximide (CHX) and SU6656 were from MedChem Express (Monmouth Junction, NJ).

Min X., Zhang X., Li Y., Cao X., Cheng H., Li Y., Li C., Kong Q., Mao Q., Peng P., Ni Y., Li J., Duan Y., Liu L, & Ding Z. (2020). HSPA12A unstabilizes CD147 to inhibit lactate export and migration in human renal cell carcinoma. Theranostics, 10(19), 8573-8590.

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Hormone Treatments and Inhibitor Assays

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Fulvestrant, 4-hydroxytamoxifen, and 17-β estradiol (E2) were purchased from Millipore Sigma (St. Louis, MO). For in vitro hormone treatments, cells were cultured in charcoal-stripped serum (Thermo Scientific, Waltham, MA) containing phenol red-free media for 3–5 days, then treated with 10nM E2 as determined by our dose response and the literature (16 (link)–18 (link)). For clonogenic and growth assays, Fulvestrant and 4-hydroxytamoxifen treatments were at a final concentration of 7.5nM and 5μM, respectively. Palbociclib and SU6656 were acquired from MedChemExpress (Monmouth Junction, NJ) and treatments were at a final concentration of 2.5μM and 5μM, respectively, in DMSO. LY294002 was purchased from Cell Signaling Technologies (Danvers, MA) and in vitro treatments were at a final concentration of 2.5μM LY294002 in DMSO. In vitro venetoclax (Abbvie, Chicago, IL) was at a final concentration of 300nM in DMSO.

Crump L.S., Wyatt G.L., Rutherford T.R., Richer J.K., Porter W.W, & Lyons T.R. (2020). Hormonal regulation of Semaphorin 7a in ER+ breast cancer drives therapeutic resistance. Cancer research, 81(1), 187-198.

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