Irtracer 100 fourier transform infrared spectrophotometer

Proton nuclear magnetic resonance (¹H NMR) spectra were recorded on an AVANCE III HD 400 MHz NMR spectrometer (Bruker) or an AVANCE III HD 500 MHz NMR spectrometer (Bruker). Carbon-13 nuclear magnetic resonance (¹³C NMR) spectra were recorded on the AVANCE III HD 400 MHz NMR spectrometer with a Cryoprobe (Bruker). Mass spectra were recorded on a solariX Fourier transform ion cyclotron resonance mass spectrometer (Bruker) or a ACQUITY UPLC system (Waters) or a GCMS-QP2010 SE gas chromatograph mass spectrometer (Shimadzu). Fourier transform infrared (FT-IR) spectra were recorded on a IRTracer-100 Fourier transform infrared spectrophotometer (Shimadzu). UV/Vis spectra were acquired on a UV-2600 UV-Vis spectrophotometer (Shimadzu). The pressure in the pressure tight vessel was measured using a Krone digital pressure gauge KDM30 (Krone).

The Fourier-Transform Infrared Spectroscopic (FT-IR) analysis was conducted to confirm the presence of functional groups in the structure of the synthesized QPABA. The FT-IR spectra of both quercetin and QPABA were recorded using the Shimadzu IRTracer-100 Fourier Transform Infrared Spectrophotometer (Shimadzu Corporation, Kyoto, Japan) equipped pyroelectric DLATGS detector and advanced dynamic alignment. The sample compartment was cleaned with deionized water and purged with dry air to minimize the interference with the absorbance of atmospheric water vapor. The spectrum of air recorded as a background signal was subtracted automatically using an in-built software of the instrument. The dried QPABA sample was crushed into fine particles and loaded unto the sample holder. The FT-IR spectral scans were recorded from 4000–400 cm⁻¹ at a resolution of 4 cm⁻¹ and 45 scans were recorded during the analysis. The dried QPABA samples were analyzed without further sample modification.