Fresh duck eggs were obtained from a Jiangxi Agricultural University farm in Jiangxi Province, China. Sodium chloride (NaCl), potassium ferrocyanide, potassium chromate, n-hexane, iso- propyl alcohol, disodium hydrogenorthophosphate, silver nitrate, sodium dihydrogen phosphate and ethanol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Zinc acetate and glutaraldehyde were purchased from Xilong Chemical CO.,Ltd. (Guangdongi, China) and Jingchun Scientific Co., Ltd. (Shanghai, China), respectively.
Potassium ferrocyanide
Manufactured by Sinopharm
Sourced in China
Potassium ferrocyanide is a chemical compound with the formula K4[Fe(CN)6]. It is a yellow crystalline solid that is soluble in water. Potassium ferrocyanide is commonly used as a laboratory reagent and in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Sinopharm (4 mentions)
Potassium ferricyanide, by Sinopharm (4 mentions)
Glutaraldehyde, by Xilong (3 mentions)
Zinc acetate, by Xilong (3 mentions)
N hexane, by Sinopharm (2 mentions)
Trichloroacetic acid, by Sinopharm (2 mentions)
Hydrochloric acid (hcl), by Sinopharm (2 mentions)
Sodium nitrite, by Sinopharm (2 mentions)
Potassium chromate, by Sinopharm (1 mentions)
Silver nitrate, by Sinopharm (1 mentions)
Isopropyl alcohol, by Sinopharm (1 mentions)
Sodium chloride (nacl), by Sinopharm (1 mentions)
Sodium dihydrogen phosphate, by Sinopharm (1 mentions)
Oxalic acid, by Sinopharm (1 mentions)
Chitosan, by Sinopharm (1 mentions)
Glycerol, by Sinopharm (1 mentions)
Polyvinylpyrrolidone, by Sinopharm (1 mentions)
Catechin, by Solarbio (1 mentions)
Phosphate buffered saline (pbs), by Sinopharm (1 mentions)
Vanillin, by Solarbio (1 mentions)
10 protocols using potassium ferrocyanide
1
Characterization of Fresh Duck Eggs
2
Strawberry Preservation with Litsea cubeba
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Strawberries were picked at market-ready maturity from the Changsha Farmers Ecological Strawberry Garden, Changsha, China. Strawberries that were regular in shape and size, weighing about 20 g each with no mechanical damage, were selected for use in the study. Litsea cubeba EO was extracted from mountain pepper grown in Yongshun, Hunan province, by steam distillation, and the oil contained 74.8 percent of citral, as determined by GC-MS. Chitosan, glycerol, polyvinylpyrrolidone, trichloroacetic acid, oxalic acid, and potassium ferrocyanide were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China) and were all analytical grade. Corn starch was purchased from Zhuzhou Xiangoose Food Company (Zhuzhou, China).
3
Phytochemical Extraction and Analysis
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All reagents used in the extractions were of analytical grade. Methanol, ethanol, n-hexane, hydrochloric acid, chloroform, anhydrous sodium carbonate, aluminum nitrate, Tris-HCl buffer, potassium ferricyanide, ferric chloride, ferrous sulfate, sodium nitrite, trichloroacetic acid, potassium persulfate, potassium ferrocyanide, and phosphate-buffered saline (PBS) were purchased from Sinopharm Chemical Reagent Co., Ltd, (Shanghai, China). Ascorbic acid, vanillin, catechin, gallic acid, chelerythrine, quercetin, Folin-Ciocalteu’s reagent, acetylthiocholine iodine (ATCI), s-butyrylthiocholine iodine (BTCI), and 5,5-dithiobis- (2-nitrobenzoic acid) (DTNB) were purchased from Solarbio Technology Co., Ltd. (Beijing, China)
4
ε-Poly-L-lysine Synthesis and Characterization
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ε-Poly-l -lysine (ε-PL, molecular weight: 3500–5000 Da) was purchased from Nanjing Bioshineking Biotech Co., Ltd. Dopamine hydrochloride (DA) and 3,4-dihydroxy benzaldehyde (DBAH) were obtained from Aladdin Industrial Corporation (China). Methanol (absolute), triethylamine, sodium hydroxide (NaOH), potassium ferricyanide (K3[Fe(CN)6]), potassium ferrocyanide (K4Fe(CN)6·3H2O) and ethanol were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the solutions were prepared in Ultrapure water (18.2 MΩ, Milli-Q, Millipore). Anti-S. aureus antibody was obtained from Abcam Inc (Cambridge, UK), and BSA was purchased from Sigma (St. Louis, MO, USA). Milk was purchased from local markets.
5
Synthesis and Characterization of Cu2O-BSA Nanoparticles
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Copper nitrate trihydrate (Cu(NO3)2⋅3H2O), sodium hydroxide (NaOH), sodium chloride (NaCl), potassium ferricyanide, potassium ferrocyanide, glucose, ethanol, AA, UA, and AP are analytical pure and were bought from Sinopharm Chemical Reagent Co., Ltd. BSA was purchased from Aladdin with purity of 96%. Nafion 117 solution is analytical purity, produced by Sigma-Aldrich Company, USA. All of reagents were used without further purification.
The crystalline structure of Cu2O–BSA NPs was detected by an X-ray diffractometer (Bruker D8 ADVANCE) with Cu-Kα radiation (λ = 1.5406 Å). A type of H-7650 TEM (Japan) was used to observe the size and morphology of the Cu2O–BSA NPs. A CHI 690 electrochemical workstation (Chenhua, Shanghai, China) was assumed to conduct the electrochemical experiments including CV and amperometry. A conventional three-electrode electrochemical system, which consists of a working electrode, a Pt foil auxiliary electrode, and Ag/AgCl reference electrode, was used for all electrochemical experiments.
The crystalline structure of Cu2O–BSA NPs was detected by an X-ray diffractometer (Bruker D8 ADVANCE) with Cu-Kα radiation (λ = 1.5406 Å). A type of H-7650 TEM (Japan) was used to observe the size and morphology of the Cu2O–BSA NPs. A CHI 690 electrochemical workstation (Chenhua, Shanghai, China) was assumed to conduct the electrochemical experiments including CV and amperometry. A conventional three-electrode electrochemical system, which consists of a working electrode, a Pt foil auxiliary electrode, and Ag/AgCl reference electrode, was used for all electrochemical experiments.
6
Electrochemiluminescent Sensor Fabrication
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Cobalt nitrate (98%), 2-methacrylic acid (MAA, 99%), ammonium fluoride (95%), ethylene glycol dimethacrylate (EDMA, 98%), acetonitrile (AR), BPS and azobisisobutylnitrile (AIBN, 98%) were supplied by Macklin (Shanghai, China). In addition, tris(2,2-bipyridyl)dichlororuthenium(ii ) hexahydrate (Ru(bpy)3Cl2·6H2O), urea (99%), potassium ferricyanide (K3[Fe(CN)6]), potassium ferrocyanide (K3[Fe(CN)6]), potassium chloride (KCl) and potassium nitrate (KNO3) were purchased from Sinopharm (Beijing, China). All the reagents used in the experiment were analytical grade reagents, and ultrapure water (18.25 MΩ cm−1) was used throughout the experiments.
The microstructure of the products was observed by scanning electron microscopy (SEM, JSM250F) and transmission electron microscopy (TEM, JEOL JEM-2100F). The X-ray diffraction (XRD) pattern was measured by the D8 focus diffractometer (Bruker AXS, Germany). The XPS analysis was performed on the Thermo ESCALAB 250XI system. The electrochemiluminescence measurements were carried out on the model MPI-F flow injection chemiluminescence detector (Remax, China). The RST 5000 electrochemical work station (Suzhou Ruisite Instrument Co, Ltd) was applied to accomplish the electrochemical impedance spectroscopy (EIS) measurements. The tube furnace that we used was acquired from Zhong yi guo ke (Beijing, China).
The microstructure of the products was observed by scanning electron microscopy (SEM, JSM250F) and transmission electron microscopy (TEM, JEOL JEM-2100F). The X-ray diffraction (XRD) pattern was measured by the D8 focus diffractometer (Bruker AXS, Germany). The XPS analysis was performed on the Thermo ESCALAB 250XI system. The electrochemiluminescence measurements were carried out on the model MPI-F flow injection chemiluminescence detector (Remax, China). The RST 5000 electrochemical work station (Suzhou Ruisite Instrument Co, Ltd) was applied to accomplish the electrochemical impedance spectroscopy (EIS) measurements. The tube furnace that we used was acquired from Zhong yi guo ke (Beijing, China).
7
Magnetic Nanoparticle-based Bacterial Detection
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Chloroauric acid trihydrate (HAuCl4), trisodium citrate, BSA, and ferric chloride (FeCl3) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS; pH 7.4, 10 mM) was obtained from Gibco (Beijing, China). Streptavidin-modified MNPs of 150-nm diameter were purchased from Ocean Nanotech (Dunedin, FL, USA). Luria–Bertani medium (LB), agar and alkaline peptone water medium were obtained from Aoboxing Biotech (Beijing, China). Escherichia coli O157:H7 (ATCC 43888), E. coli (ATCC 25922), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 25923) and Vibrio parahemolyticus (ATCC 17802) were purchased from Solarbio Life Sciences (Beijing, China). Potassium ferrocyanide and hydrochloric acid were obtained from Sinopharm (Shanghai, China). Rabbit antibody against E. coli O157:H7 was purchased from Meridian Life Science (Memphis, TN, USA). A Long-arm Biotin Labeling Kit from Elabscience Biotechnology (Wuhan, China) was used for the modification of antibodies. Ultrapure water was prepared using a Milli-Q system (Bedford, MA, USA).
8
Analytical Biosensing Assay Development
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HEX, DES, DE and BPA were purchased from Nanjing Wanqing Chemical Glassware Instrument Co., Ltd. Freund's complete and incomplete adjuvants, polyethylene glycol (PEG, Mw 1450), 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP), horse radish peroxidase (HRP), hypoxanthine–thymidine (HT) Media Supplement (50×) Hybri-Max™, hypoxanthine–aminopterin–thymidine (HAT) Media Supplement (50×) HybriMax™, 3,3′,5,5′-tetramethylbenzidine (TMB) liquid substrate, mercapto acetic acid (MACA), acetonitrile, acetone, and n-hexane were supplied by Sigma-Aldrich. Dulbecco's modified Eagle medium (DMEM), foetal bovine serum (FBS) and bovine serum albumin (BSA, further purified fraction V, approx. 99%) were obtained from Gibco BRL, USA. HRP-conjugated polyclonal goat anti-mouse IgG and IgM antibodies were purchased from Boster Biological Technology Co., Ltd. N-Hydroxysuccinimide (NHS), carbodiimide hydrochloride (EDC), gold chloride tetrahydrate (HAuCl4·4H2O), potassium ferricyanide, and potassium ferrocyanide were obtained from Sinopharm Chemical Reagent Co., Ltd. Phosphate buffer solution (PBS, 0.01 M, pH = 7.4) was prepared from potassium dihydrogen phosphate and sodium hydroxide. All other chemicals and reagents were of analytical grade. Redistilled water was used in all of the experiments. Beef, duck and the milk powder samples were purchased from a local supermarket.
9
Electrochemical Sensing of Dopamine and Uric Acid
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Dopamine (DA), uric acid (UA), Iron (III) nitrate nonahydrate (Fe(NO3)3·9H2O), cupric acetate anhydrous (Cu(Ac)2), Zinc acetate (Zn(Ac)2·2H2O), aluminum acetate (Al(Ac)3), disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O) and Sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O) were purchased from Aladdin Reagents Co., Ltd. (Shanghai, China). Graphite powder, potassium permanganate (KMnO4), hydrogen peroxide (H2O2, 30%), sodium nitrate (NaNO3), potassium ferricyanide(K3[Fe(CN)6]), potassium ferrocyanide (K4[Fe(CN)6]), ammonia (NH3·H2O), sodium hydrate (NaOH), concentrated hydrochloric acid (HCl, 37%), concentrated sulfuric acid (H2SO4, 98%) and absolute ethanol (CH3CH2OH) were supplied by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). All chemicals were analytically pure and directly used as received. Human serum samples were provided by Zhuzhou People’s Hospital (Zhuzhou, China). The stock solutions of DA and UA (1 mM) were prepared by dissolving appropriate amount of DA and UA in 500 mL 0.1 M PBS. Then lower concentration series of the standard solution were obtained by appropriately diluting the stock solution with 0.1 M PBS. Deionized water (DI water, 18.2 MΩ) was used in all the experiments.
10
Extraction and Characterization of Rape Bran Polyphenols
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Commercial bread flour (Angel Yeast Co., Ltd., Yichang, China), instant yeast, sugar, salt, eggs, and butter for bread making were purchased from a local supermarket in Beijing. The dry rape BP was obtained from Sichuan Kuake Technology Development Co., Ltd. (Sichuan Province, China) without mildew and impurities. Sodium hydroxide, anhydrous ethanol, acetic acid, and petroleum ether were purchased from Beijing Chemical Industry Group Co., Ltd. (Beijing, China). Potassium ferrocyanide, ammonium molybdate, sodium acetate, sodium nitrite, and Folin-Ciocalteu phenol reagent were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Aluminium nitrate and gallic acid were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Rutin, α-amylase, neutral protease, glucoamylase, and diphenylpicrylhydrazyl (DPPH) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). All these chemicals were of analytical grade unless otherwise stated.
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