(+)-Pentazocine, haloperidol, and buffer components (TRIS-HCl, inhibitors) were purchased from Sigma-Aldrich Kft. (Budapest, Hungary). The radioligand [3H]-(+)-pentazocine (s.a. 1.98 TBq/mmol) was prepared in the Laboratory of Chemical Biology (BRC, Szeged, Hungary). Tritium labeling was carried out in a self-designed vacuum manifold [68 (link)] and radioactivity was measured with a Packard Tri-Carb 2100 TR liquid scintillation analyzer (Packard, Perkin Elmer, Waltham, MA, USA) using Insta Gel scintillation cocktail of PerkinElmer. Drugs were dissolved at 1 mM in dimethyl sulfoxide (DMSO) and stored at −20 °C, and then diluted in the binding buffer. 2,4,6-trinitrobenzenesulfonic acid (TNBS) was purchased from Sigma-Aldrich (Budapest, Hungary), and was dissolved in 50% ethanol and distilled water mixture. Fluvoxamine (Fluvoxamine maleate, Sigma-Aldrich, Budapest, Hungary) was dissolved in 3% dimethyl sulfoxide (DMSO). BD1063, purchased from Tocris (Bio-Techne R&D Systems Kft., Budapest, Hungary), was dissolved in physiological saline (0.9%). Sulfasalazine (SASP) was used as a positive control and purchased from Sigma-Aldrich (Budapest, Hungary). The anesthetic agent Thiopental (Tiobarbital Braun, 0.5 g, B. Braun Medical SA, Barcelona, Spain) was dissolved in saline (0.9%).
Sulfasalazine sasp
Manufactured by Merck Group
Sourced in United States
Sulfasalazine (SASP) is a chemical compound used in the production of laboratory equipment. It is a stable, crystalline substance that serves as a key component in various analytical and experimental procedures. SASP is utilized for its specific chemical properties and functionality, though its exact intended use may vary depending on the specific application and laboratory requirements.
Automatically generated - may contain errors
Lab products found in correlation
Hematoxylin, by Merck Group (2 mentions)
Doxorubicin, by Merck Group (2 mentions)
2 4 6 trinitrobenzenesulfonic acid tnbs, by Merck Group (1 mentions)
Fluvoxamine fluvoxamine maleate, by Merck Group (1 mentions)
Insta gel scintillation cocktail, by PerkinElmer (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 cell culture medium, by Thermo Fisher Scientific (1 mentions)
Antibodies against cox 2, by Cell Signaling Technology (1 mentions)
Fitc conjugated donkey anti rabbit igg antibody, by Santa Cruz Biotechnology (1 mentions)
Il 6 elisa kit, by Thermo Fisher Scientific (1 mentions)
O dianisidine dihydrochloride, by Merck Group (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Hexadecyltrimethylammonium bromide, by Merck Group (1 mentions)
7 protocols using sulfasalazine sasp
1
Radioligand Binding Assay with Pentazocine
2
Dextran Sulfate Sodium-Induced Colitis Model
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DSS (molecular weight: 36,000–50,000 Daltons) was purchased from MP Biologicals (Santa Ana, USA). Dimethylsulphoxide (DMSO), lipopolysaccharide (LPS) (Escherichia coli Serotype 055:B5), sulfasalazine (SASP) (purity ≥ 98 %), hexadecyltrimethylammonium bromide, hematoxylin, eosin, O-dianisidine dihydrochloride, protease inhibitor cocktails and hydrogen peroxide were purchased from Sigma-Aldrich (St, Louis, MO, USA). Fetal bovine serum, l -glutamine, penicillin, streptomycin and RPMI 1640 cell culture medium were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against COX-2, iNOS, IκB α and p65 and β-actin were supplied from Cell Signaling Technology, Inc. (Beverly, MA, USA). TNF-α, IL-1β, and IL-6 ELISA kits were purchased from eBioscience (San Diego, CA, USA). Cy5.5 PerCP anti-mouse CD11b, FITC anti-mouse F4/80 were purchased from BD Pharmingen (San Diego, CA, USA). FITC-conjugated donkey anti-rabbit IgG antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). WesternBright™ ECL was supplied from Advansta (Menio Park, CA, USA).
3
Kumatakenin and Sulfasalazine Study
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Kumatakenin (PubChem CID: 5318869) of ≥95% purity and sulfasalazine (SASP, PubChem CID: 5359476) were obtained from Sigma-Aldrich (St. Louis, MO, United States of America). Anti-enolase (Eno3, ab232759), anti-iron regulatory protein (IRP1, ab183721), anti-4-HNE (ab48506), and anti-GAPDH (ab9485) antibodies were obtained from Abcam (Hong Kong, China). Other reagents were purchased from Sigma-Aldrich.
4
Inflammatory Bowel Disease Model Protocol
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BLG granule (without sugar, National medicine approval Z11020357; Beijing Tongrentang Science and Technology Development Co., Ltd, Beijing, China; batch number: 20110966) was purchased from the drugstore. DSS (molecular weight: 36,000–50,000 Daltons) was obtained from MP Biologicals (Santa Ana, USA). Sulfasalazine (SASP) (purity ≥ 98%), hematoxylin, and eosin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse TNF-α, IL-1β, and IL-6 luminex Elisa assay kit was purchased from R&D systems (Minneapolis, MN, USA). Acetic acid, propionic acid, and butyric acid were purchased from Aladdin Industrial Corporation (Shanghai, China). 2-ethylbutyric acid was bought from Merck KGaA (Darmstadt, Germany).
5
Alhagi Herb Anti-Inflammatory Effects
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The herb Alhagi was obtained from the Turpan Depression (Xinjiang, China) and identified by Professor Yonghe Li (College of Traditional Chinese Medicine, Xinjiang Medical University, Xinjiang, China). Specimens of the herbs (NO: LTC_20190612) were stored in the herbarium center of the Xinjiang Institute of Chinese MateriaMedica and Ethnodrug. Dextran sulfate sodium (DSS; No.0216011080, 36,000–50,000Da) was purchased from MP Biomedicals and dissolved in 0.9% NaCl to a final 2.5%. Chromatographic grade methanol, acetonitrile, and formic acid were obtained from CNW Technologies (Shanghai, China). Sulfasalazine (SASP) was purchased from Sigma Aldrich (St. Louis, MO, United States). SuperSignal™ West Pico PLUS and Chemiluminescent Substrate were purchased from Thermo Fisher Scientific. Antibodies against TLR4 (Cat.No.bs-20594R), NF-κBp65 (Cat.No.bs-0465R), p-NF-κB p65 (Ser536) (Cat.No.bs-0982R), and IK-Kβ (Cat.No.bs-4880R) were purchased from Beijing Boaosen Biotechnology Co., Ltd. (Beijing, China). The antibody p-IKKα/β (Ser176/180) (Cat. No. 2694S) was purchased from Cell Signaling Technology. Goat Anti-Mouse or Anti-Rabbit IgG was purchased from Abcam. A mouse IL-1β, IL-6, IL-10, and TNF-α ELISA kits were purchased from Lianke Biotechnology Co., Ltd. (Xinjiang, China).
6
Multimodal Cellular Characterization Assay
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Hoechst 33342 (H3570, 1:500), 2′,7′-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA. C6827, 1:1000), ThiolTracker™ violet (T10095, 1:500), Alexa Fluor® 633 Phalloidin (A22284, 1:100), Goat anti-mouse Alexa Fluor® 488 (A11001, 1:500), Goat anti-rabbit Alexa Fluor® 633 (A21070, 1:500), Goat anti-mouse Alexa Fluor® 633 (A21050, 1:500) and Goat anti-rabbit Alexa Fluor® 488 (A11008, 1:500) were purchased from Molecular Probes (Invitrogen). Rabbit monoclonal anti-CD44 (EPR10133Y clone, ab51037, 1:1000) rabbit polyclonal anti-EpCAM (ab71916, 1:1000) and mouse monoclonal anti-CD90 (ab133350, 1:1000) were purchased from Abcam (CSP, Cambridge, England). Methotrexate, Doxorubicin, Cisplatin, Sorafenib, Sulfasalazine (SASP), Buthionine sulphoximine (BSO), Arsenic trioxide, Etoposide and Hydrogen peroxide (H2O2) were purchased from Sigma Chemicals. Mouse monoclonal anti-CD133/1 (AC133, 130-090-422, 1:100) was purchased from Miltenyi biotec (Bergisch Gladbach, Germany). Rabbit polyclonal anti-AFP (Dako, Denmark A/S, Denmark, A000829. 1:500) and mouse monoclonal anti-β-actin (Sigma, A5441, 1:10,000) antibodies were purchased from each of the indicated companies.
7
Maintaining PEL Cell Lines for Research
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The PEL cell-line BCBL-1 (KSHV+/EBVneg) and a Burkitt's lymphoma cell line BL-41 (KSHVneg/EBVneg) was kindly provided by Dr. Dean Kedes (University of Virginia) and maintained in RPMI 1640 medium (Gibco) with supplements as described previously [42 (link)]. The other PEL cell-lines BC-1 (KSHV+/EBV+) and BCP-1 (KSHV+/EBVneg) were purchased from American Type Culture Collection (ATCC) and maintained in complete RPMI 1640 medium (ATCC) supplemented with 20% FBS. All cells were cultured at 37°C in 5% CO2. All experiments were carried out using cells harvested at low (< 20) passages. Sulfasalazine (SASP) and Doxorubicin were purchased from Sigma.
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