We performed the pulsed-field gel electrophoresis (PFGE) analysis using the Bio-Rad system and slightly modified the protocol according to the Tanimoto’s and Shueh’s reports [14 (link), 16 (link)]. We conducted the preliminary experiments as follows: Restriction enzyme Xbal (Roche Diagnostics, Basel, Switzerland) was digested at 37 °C for 3 h. The PGFE electrophoresis conditions followed the manufacturer’s protocol: 2000 mL 0.5 *TBE, voltage of 6.0 V max, temperature of 14 °C, pulse angle of 120, start pulse time of 5 s, end pulse time 20 s, and an electrophoresis time of 19 h. We analyzed the results using BioNumerics software (version 4.0, Applied Maths, Sint-Martens-Latem, Belgium). The Salmonella serotype Braenderup strain (H9812) was the molecular weight marker.
Restriction enzyme xbal
Manufactured by Roche
Sourced in Switzerland
Restriction enzyme XbaI is a type II restriction endonuclease that recognizes and cleaves the palindromic DNA sequence 5'-TCTAGA-3' (or its reverse complement 5'-AGATCT-3'). It is commonly used in molecular biology for DNA manipulation and analysis.
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Lab products found in correlation
2 protocols using restriction enzyme xbal
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Optimized PFGE Analysis of Salmonella Serotypes
2
Pulsed-Field Gel Electrophoresis for Bacterial Fingerprinting
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The PFGE was performed using the Bio-Rad system, and protocol was slightly modified according to the Tanimoto's and Shueh CS's reports [14, 16] and our preliminary experiments: Restriction enzyme Xbal (Roche Diagnostics) was digested at 37 °C for 3 hours; PGFE electrophoresis conditions followed the manufacturer's protocol: 2000 mL 0.5 *TBE, voltage 6.0 V max, temperature 14 °C, pulse angle 120, start pulse time 5 s, end pulse time 20 s, electrophoresis time 19 hours. The results were analyzed using BioNumerics software (version 4.0, Applied Maths, Belgium). Salmonella serotype Braenderup strain (H9812) was molecular weight Marker.
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