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3 protocols using na2so4

1

Recombinant Protein Production in E. coli

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BP1 was produced by a fermentation process using E.coli, which was previously reported in the literature28 ,44 and provided in an unprocessed powder form by Spiber Inc. The amino acid composition of BP1 was alanine (16.4%), tyrosine (12.0%), glutamine (23.7%), glycine (21.9%), proline (15.3%), serine (9.3%) and others (1.4%). The protein sequence structure is shown in Fig. 1.
KH2PO4, K2HPO4, NaCl, Na2HPO4·H2O, NH4Cl, MgCl2·6H2O, CaCl2, FeCl3·6H2O, Yeast extract, and agar powder were purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Na2SO4, KCl, HCl, Na2SO4, NaHCO3, and NaOH were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Plysurf was purchased from DKS Co. Ltd. (Kyoto, Japan). All chemicals were of reagent grade and used without further purification. Pronase E (P5147 Protease Type XIV from Streptomyces griseus) was purchased from Sigma Aldrich, Proteinase K was purchased from TaKaRa Bio Inc (Kusatsu, Japan), and chymotrypsin was purchased from NACALAI TESQUE, INC (Kyoto, Japan).
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Enzymatic Assay for Lysyl Oxidase

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Poly-L-lysine hydrobromide (MW, 70,000–150,000 Da), poly-(D,L)-lysine hydrobromide (MW, 25,000–40,000 Da), and 2,6-dichloroindophenol sodium salt hydrate (DCIP) were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). NaCl, (NH4)2SO4, Na2SO4, NaSCN, and dimethyl sulfoxide (DMSO) were obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). Tris(hydroxymethyl)aminomethane was obtained from Nacalai Tesque (Kyoto, Japan). 2-Morpholinoethanesulfonic acid monohydrate and 3-[4-(2-Hydroxyethyl)-1-piperazinyl] propanesulfonic acid (EPPS) were obtained from Dojindo Laboratories (Kumamoto, Japan). l-lactic acid was obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Rhodamine B isothiocyanate (RBITC) was obtained from Santa Cruz Biotechnology (Dallas, TX). LOX was prepared as previously described46 (link).
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3

Photocatalytic Hydrogen Production from Nanosheets

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Photocatalytic reactions were performed in an air-free, glass, closed circulation system. A top-irradiation type reaction cell made of Pyrex glass was used, combined with a cooling water bath to maintain the temperature of the reactor at approximately 293 K throughout the reaction. In each reaction, a 50 mg quantity of the restacked nanosheets was suspended in an aqueous solution (100 mL) containing various salts [NaI (≥99.5%, Kanto Chemical Co.), KI (≥99.5%, Kanto Chemical Co.), Na2SO4 (≥99.0%, Kanto Chemical Co.), or K2SO4 (≥99.0%, Kanto Chemical Co.)]. After degassing the reaction cell, a small amount (ca. 3 kPa) of argon gas was introduced and the suspension was irradiated using a 300 W xenon lamp (λ ≥ 300 nm) to photoexcite KCa2Nb3O10 that has a band gap of ca. 3.6 eV. The light intensity at 350 nm was approximately 1.1 × 10−3 einstein per h. The evolved gases were analyzed by gas chromatography (Shimadzu, GC-8A with a TCD detector and an MS-5A column, argon carrier gas).
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