Mk886

Manufactured by Enzo Life Sciences

MK886 is a laboratory equipment product manufactured by Enzo Life Sciences. It is a selective and potent inhibitor of 5-lipoxygenase-activating protein (FLAP), an enzyme involved in the biosynthesis of leukotrienes. The core function of MK886 is to inhibit FLAP activity in experimental settings.

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Lab products found in correlation

U0126, by Cell Signaling Technology (1 mentions) Zileuton, by Merck Group (1 mentions) Ns 398, by Enzo Life Sciences (1 mentions)

2 protocols using mk886

Neutrophil Inhibition Prior to Infection

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Neutrophil inhibitor treatments were performed immediately prior to infection. Neutrophils (2.5x10⁷/mL) were incubated with 20μM U0126 (Cell Signaling Technology), or 2μM MK886 (Enzo Life Sciences) for 1h at 37°C in HBSS-. After drug treatment, neutrophils were resuspended in HBSS+ containing inhibitors at 5x10⁶/mL, and promptly utilized in the transepithelial migration assay. Neutrophil viability following drug treatment was assessed by trypan blue exclusion. No evidence of decreased viability was observed compared to vehicle-treated or untreated neutrophil controls.

Pazos M.A., Lanter B.B., Yonker L.M., Eaton A.D., Pirzai W., Gronert K., Bonventre J.V, & Hurley B.P. (2017). Pseudomonas aeruginosa ExoU augments neutrophil transepithelial migration. PLoS Pathogens, 13(8), e1006548.

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Epithelial and Neutrophil Drug Treatments for Migration

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Epithelial drug treatments were performed immediately prior to infection. Epithelial cells were washed three times, and incubated with zileuton (Sigma-Aldrich; St. Louis, MO), CDC (Enzo Life Sciences), MK886 (Enzo Life Sciences), or NS398 (Enzo Life Sciences) at the indicated concentration for 1h at 37°C in HBSS. After drug treatment, epithelial cells were washed three times then infected, as above. Untreated neutrophils were then added for migration. In experiments calling for drug-treatment of neutrophils, neutrophils (5×10⁷/mL) were suspended in zileuton, CDC, MK886 or vehicle controls at the indicated concentrations for 1h at 37°C in HBSS−. Immediately prior to migration, neutrophils were resuspended in HBSS containing inhibitors. Neutrophil viability was confirmed by trypan blue exclusion.

Pazos M.A., Pirzai W., Yonker L.M., Morisseau C., Gronert K, & Hurley B.P. (2014). Distinct cellular sources of Hepoxilin A3 and Leukotriene B4 are used to coordinate bacterial-induced neutrophil transepithelial migration. Journal of immunology (Baltimore, Md. : 1950), 194(3), 1304-1315.

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