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Sod2 assay kit

Manufactured by Beyotime
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Sourced in China

The SOD2 assay kit is a laboratory equipment designed to measure the activity of the superoxide dismutase 2 (SOD2) enzyme. SOD2 is an important antioxidant enzyme that plays a crucial role in protecting cells from oxidative stress. The kit provides a reliable and efficient method for quantifying SOD2 activity in various biological samples.

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Lab products found in correlation

Hrp conjugated secondary antibody, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Mda assay kit, by Beyotime (2 mentions) Atp assay kit, by Beyotime (2 mentions) Mitochondrial isolation kit, by Beyotime (1 mentions) Mitosox, by Merck Group (1 mentions) Antibodies against chop, by Cell Signaling Technology (1 mentions) T sod assay kit, by Beyotime (1 mentions) Mitotempo, by Merck Group (1 mentions) Cellular glutathione peroxidase 1 assay kit, by Beyotime (1 mentions) Cox 4, by Cell Signaling Technology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) Sqstm1, by Abcam (1 mentions) Pgc 1α, by Abcam (1 mentions) Pro il 1β, by Abcam (1 mentions) Il 1β, by Abcam (1 mentions) Nlrp3 and asc, by Adipogen (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)

4 protocols using sod2 assay kit

1

Mitochondrial Oxidative Stress Assays

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Mito-TEMPO and MitoSOX were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against CHOP, BiP, GRP94, β-actin, and HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against SOD2 and caspase-12 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against Sirt3 and acetyl-K68-SOD2 were obtained from Abcam (Cambridge, MA, USA). T-SOD assay kit, SOD2 assay kit, MDA assay kit, ATP assay kit, and mitochondrial isolation kit were purchased from Beyotime Institute of Biotechnology (Jiangsu, China).
Liu Y., Wang Y., Ding W, & Wang Y. (2018). Mito-TEMPO Alleviates Renal Fibrosis by Reducing Inflammation, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress. Oxidative Medicine and Cellular Longevity, 2018, 5828120.
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2

Assay of SOD2 Activity in Intestine

Cited 1 time
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SOD2 enzymatic activities in small intestinal tissue were analysed using a SOD2 assay kit (Beyotime Institute of Biotechnology, Jiangsu, China), respectively, following the manufacturer’s instructions [10 (link)].
Zhang H., Yan H., Zhou X., Wang H., Yang Y., Zhang J, & Wang H. (2017). The protective effects of Resveratrol against radiation-induced intestinal injury. BMC Complementary and Alternative Medicine, 17, 410.
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3

Enzymatic Activities of SOD2 and GPX1

Cited 1 time
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The enzymatic activities of SOD2 and GPX1 in BMMNC were analyzed using a SOD2 assay kit and a Cellular Glutathione Peroxidase 1 assay kit (Beyotime Institute of Biotechnology, Jiangsu, China); these assays were performed following the manufacturer's instruction, as described previously [9 (link)].
Wang H., Yang Y.L., Zhang H., Yan H., Wu X.J, & Zhang C.Z. (2014). Administration of the Resveratrol Analogues Isorhapontigenin and Heyneanol-A Protects Mice Hematopoietic Cells against Irradiation Injuries. BioMed Research International, 2014, 282657.
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4

Antibody-Guided Protein Analysis

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Antibodies recognizing the following proteins were used in this study: caspase-3, Bax, LC3, ATG7, COXIV, β-actin, and HRP-conjugated secondary antibodies (Cell Signaling Technology, Beverly, MA); pro-IL-1β, IL-1β, PGC-1α, TFAM, and SQSTM1 (Abcam, Cambridge, MA); MyHC (R&D Systems, Minneapolis, MN); Nlrp3 and ASC (AdipoGen, San Diego, CA); pro-IL-18 and IL-18 (MBL, Minneapolis, MN); and procaspase-1 and caspase-1 (Santa Cruz Biotechnology, Santa Cruz, CA). The SOD2 assay kit, MDA assay kit, and ATP assay kit (Beyotime Biotechnology, China) were also used.
Zhang Y., Liu Y., Bi X., Hu C., Ding F, & Ding W. (2019). Therapeutic Approaches in Mitochondrial Dysfunction, Inflammation, and Autophagy in Uremic Cachexia: Role of Aerobic Exercise. Mediators of Inflammation, 2019, 2789014.
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