A549, NCI-H1650, HCC827, and NCI-H358 cells were cultured on Falcon chamber slides (BD Biosciences, USA) until 50-60% confluence before being fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton X-100. Cells were immersed three times with cold phosphate-buffered saline (PBS), incubated with FOXM1 (Santa Cruz Biotechnology, USA), E-cadherin, N-cadherin, vimentin (Abcam, Cambridge, UK), and SNAIL (Abgent, USA) primary antibodies at 4°C overnight, subsequently incubated with corresponding Alexa Fluor-conjugated secondary antibodies (Life Technologies, USA) at room temperature for 1 h, and mounted using ProLong® Gold Antifade Reagent with DAPI (Life Technologies, USA). Microscopic images of cells were obtained using a Leica inverted fluorescence microscope (Leica Microsystems, Wetzlar, Germany) with ProgRes Image Capture Software (JENOPTIK Optical Systems, Jena, Germany) and a Leica Confocal LAS-AF SP5 System (Leica Microsystems, Germany).
Falcon chamber slides
Manufactured by BD
Sourced in United States
Falcon chamber slides are a type of laboratory equipment designed for cell culture and microscopy applications. They provide a contained environment for culturing and observing cells. The slides feature multiple chambers that can hold different cell samples or solutions, allowing for efficient parallel experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
Inverted fluorescence microscope, by Leica (1 mentions)
Confocal las af sp5 system, by Leica (1 mentions)
Snail, by Abcepta (1 mentions)
Foxm1, by Santa Cruz Biotechnology (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Vimentin, by Abcam (1 mentions)
E cadherin, by Abcam (1 mentions)
N cadherin, by Abcam (1 mentions)
Progres image capture software, by Jenoptik (1 mentions)
Anti taz, by Cell Signaling Technology (1 mentions)
Anti yap, by Cell Signaling Technology (1 mentions)
2 protocols using falcon chamber slides
1
Immunofluorescence Analysis of EMT Markers
2
Subcellular Localization of YAP/TAZ
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Transfected PANC-1 cells were then further cultured on Falcon chamber slides (BD Biosciences) at up to 30–40% confluence before being fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton X-100. The cells were then immersed three times in phosphate-buffered saline, incubated with indicated anti-YAP (#12395, Cell Signaling Technology) and anti-TAZ (#4883, Cell Signaling Technology) primary antibody overnight at 4 °C and corresponding Alexa Fluor-conjugated secondary antibodies (Invitrogen) for 1 h at room temperature, and mounted using a mounting medium containing 4′,6- diamidino-2-phenylindole. Microscopic images of cells were obtained using an Axio Observer A microscope (Zeiss). The experiments were performed in triplicate and repeated twice.
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