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Astrocyte differentiation and maturation media

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Astrocyte Differentiation and Maturation media is a cell culture medium designed to support the differentiation and maturation of astrocytes, which are a type of glial cell found in the central nervous system. The medium provides the necessary nutrients and growth factors to facilitate the development of astrocytes from their progenitor cells.

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Lab products found in correlation

Neural induction medium, by STEMCELL (2 mentions) Neuron differentiation and maturation kit, by STEMCELL (2 mentions) Neural progenitor medium, by STEMCELL (2 mentions) Mtesr1 medium, by STEMCELL (2 mentions)

2 protocols using astrocyte differentiation and maturation media

1

Differentiation of iPSCs into NPCs and Neurons

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The normal human-induced pluripotent stem cells (iPSCs) were obtained from the ATCC (#ACS-1011) and cultured in mTeSR1 medium (StemCell Technologies, #05850). Human iPSCs were differentiated into NPCs using a neural induction medium (StemCell Technologies, #05835), and NPCs were maintained in Neural Progenitor Medium (StemCell Technologies, #05833). An additional NPC line (StemCell Technologies, #70901) was ordered for cell proliferation and neuronal differentiation assays. Neuronal differentiation was induced by a Neuron Differentiation and Maturation Kit (StemCell Technologies, #08500, #08510). For astrocyte differentiation, NPCs were plated on Matrigel-coated culture dish, then incubated in Astrocyte Differentiation and Maturation media (StemCell Technologies, #08540, #08550).
Ding C., Zhang C., Kopp R., Kuney L., Meng Q., Wang L., Xia Y., Jiang Y., Dai R., Min S., Yao W.D., Wong M.L., Ruan H., Liu C, & Chen C. (2020). Transcription factor POU3F2 regulates TRIM8 expression contributing to cellular functions implicated in schizophrenia. Molecular psychiatry, 26(7), 3444-3460.
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2

Differentiation of iPSCs into NPCs and Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
The normal human-induced pluripotent stem cells (iPSCs) were obtained from the ATCC (#ACS-1011) and cultured in mTeSR1 medium (StemCell Technologies, #05850). Human iPSCs were differentiated into NPCs using a neural induction medium (StemCell Technologies, #05835), and NPCs were maintained in Neural Progenitor Medium (StemCell Technologies, #05833). An additional NPC line (StemCell Technologies, #70901) was ordered for cell proliferation and neuronal differentiation assays. Neuronal differentiation was induced by a Neuron Differentiation and Maturation Kit (StemCell Technologies, #08500, #08510). For astrocyte differentiation, NPCs were plated on Matrigel-coated culture dish, then incubated in Astrocyte Differentiation and Maturation media (StemCell Technologies, #08540, #08550).
Ding C., Zhang C., Kopp R., Kuney L., Meng Q., Wang L., Xia Y., Jiang Y., Dai R., Min S., Yao W.D., Wong M.L., Ruan H., Liu C, & Chen C. (2020). Transcription factor POU3F2 regulates TRIM8 expression contributing to cellular functions implicated in schizophrenia. Molecular psychiatry, 26(7), 3444-3460.
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