The cancer cell lines (COLO-829 ATCC CRL-1974, COLO-829BL ATCC CRL-1980, HCC-1143 ATCC CRL-2321, HCC-1143 BL ATCC CRL-2362, HCC-1187 ATCC CRL-2322 and HCC-1187BL ATCC CRL-2323) were obtained from ATCC18 . The cell lines were cultured using the recommendations from ATCC. Cultured cells were split into two aliquots for metaphase chromosome preparation and karyotype analysis. Representative images and karyotypes are reported in Supplemental Fig. S1 . The number of passages is indicated in Supplemental Table 1 .
Hcc 1143 bl
Manufactured by American Type Culture Collection
The HCC-1143 BL is a human breast cancer cell line that can be used for in vitro research purposes. It serves as a model system for the study of breast cancer biology and the evaluation of potential therapeutic agents.
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Lab products found in correlation
Hcc 1187bl, by American Type Culture Collection (2 mentions)
Hcc38 bl, by American Type Culture Collection (2 mentions)
Hcc1954 bl, by American Type Culture Collection (2 mentions)
Hcc1187, by American Type Culture Collection (2 mentions)
Hcc1143, by American Type Culture Collection (2 mentions)
Hcc1395, by American Type Culture Collection (2 mentions)
Hcc1954, by American Type Culture Collection (2 mentions)
Colo829, by American Type Culture Collection (1 mentions)
Colo829bl, by American Type Culture Collection (1 mentions)
Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Dneasy blood and tissue kit, by Qiagen (1 mentions)
Hcc38, by American Type Culture Collection (1 mentions)
Hcc1937, by American Type Culture Collection (1 mentions)
3 protocols using hcc 1143 bl
1
Karyotyping Cancer Cell Lines
2
Breast Cancer Cell Line DNA Extraction
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Original cell line stocks for HCC38, HCC1143, HCC1187, HCC1954, HCC1937 and HCC1395 were obtained directly from the American Type Culture Collection (ATCC), cultured according to Neve et al [17 (link)], and passaged minimally prior to harvesting for the present study. For each respective cell line, normal matched DNA derived from B lymphoblastoid cell lines (HCC38 BL, HCC1143 BL, HCC1187 BL, HCC1954 BL, HCC1937 BL, HCC1395 BL) were purchased from ATCC. Breast cancer c ells were harvested at approximately 75% confluency and genomic DNA was isolated using DNeasy Blood and Tissue Kit (Qiagen) according to the standard manufacturer protocol. The concentration of DNA was measured with the ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies).
3
Preparation and Manipulation of B Lymphoblast Cell Lines
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Cell lines HCC1143BL, HCC1954BL, HCC1395BL, HCC38BL, and HCC1954 (Gazdar et al., 1998) were purchased from ATCC and cultured in RPMI-1640 containing penicillin-streptomycin and 10% FBS. For experiments, B lymphoblast cell lines were used at a concentration of approximately 1E6 cells/ml.
For certain experiments using B lymphoblasts, cells were pre-treated with protein degradation inhibitors or vehicle for times indicated, and cell surface MHC Class I removed by acid stripping. This was accomplished by bathing approximately 2E7 cells in 10 mL acid stripping buffer (0.132M citric acid, 0.06M sodium phosphate, pH 3.0) for 2 min on ice, followed by neutralization in 40 mL ice cold medium. Cells were washed once in medium prior to experimental treatment.
Cells were collected for mass spec by centrifugation, which was preceded by trypsin detachment for adherent cell lines (HCC1954). Cell pellets were washed once in PBS and cell count and viability was assessed using a Vi-CELL XR.
For certain experiments using B lymphoblasts, cells were pre-treated with protein degradation inhibitors or vehicle for times indicated, and cell surface MHC Class I removed by acid stripping. This was accomplished by bathing approximately 2E7 cells in 10 mL acid stripping buffer (0.132M citric acid, 0.06M sodium phosphate, pH 3.0) for 2 min on ice, followed by neutralization in 40 mL ice cold medium. Cells were washed once in medium prior to experimental treatment.
Cells were collected for mass spec by centrifugation, which was preceded by trypsin detachment for adherent cell lines (HCC1954). Cell pellets were washed once in PBS and cell count and viability was assessed using a Vi-CELL XR.
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