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Cd90.1 ox 7

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CD90.1 (OX-7) is a laboratory reagent used for the identification and study of cells expressing the CD90 (Thy-1) antigen. It is a surface marker commonly used to identify mesenchymal stem cells, certain subsets of T cells, and other cell types. The reagent can be used in flow cytometry and other immunological techniques to detect and analyze CD90-positive cells.

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Lab products found in correlation

Cd45.1 a20, by BioLegend (3 mentions) Sytox green, by Thermo Fisher Scientific (3 mentions) Cd8 β yts156.7.7, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Collagen 4, by Merck Group (1 mentions) B220 ra3 6b2, by BioLegend (1 mentions) Cd4 gk1, by BD (1 mentions) Cd8β yts156, by BioLegend (1 mentions) Bovine anti goat, by Jackson ImmunoResearch (1 mentions) Foxp3 staining buffer, by Thermo Fisher Scientific (1 mentions) Pd 1 rmp1 30, by BD (1 mentions) Fortessa flow cytometer, by BD (1 mentions)

Close spelling variants of this product

CD90-1 (clone OX-7, (2 citations)

4 protocols using cd90.1 ox 7

1

Immunostaining of Tumor-Infiltrating Cells

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GL261 or 005 tumors were harvested, then fixed in 2% paraformaldehyde for 2 h before being treated with 30% sucrose overnight for cryoprotection. The sucrose-treated tissue was embedded in OCT tissue-freezing medium and frozen in an isopentane liquid bath. Frozen blocks were processed, stained, and imaged including staining with antibodies to CD8-β (YTS156.7.7; BD Biosciences), CD90.1 (OX-7; BD Biosciences), and CD45.1 (A20; Biolegend). Sections were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) to detect nuclei.
Ning J., Gavil N.V., Wu S., Wijeyesinghe S., Weyu E., Ma J., Li M., Grigore F.N., Dhawan S., Skorput A.G., Musial S.C., Chen C.C., Masopust D, & Rosato P.C. (2022). Functional virus-specific memory T cells survey glioblastoma. Cancer immunology, immunotherapy : CII, 71(8), 1863-1875.
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2

Immunophenotyping B16 Tumor Infiltrates

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B16 tumors were harvested, then fixed in 2% paraformaldehyde for 2 h before being treated with 30% sucrose overnight for cryoprotection. The sucrose-treated tissue was embedded in OCT tissue-freezing medium and frozen in an isopentane liquid bath. Frozen blocks were processed, stained, and imaged including staining with antibodies to CD8-β (YTS156.7.7; BD Biosciences), CD90.1 (OX-7; BD Biosciences), and CD45.1 (A20; Biolegend). We also counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) to detect nuclei.
Rosato P.C., Wijeyesinghe S., Stolley J.M., Nelson C.E., Davis R.L., Manlove L.S., Pennell C.A., Blazar B.R., Chen C.C., Geller M.A., Vezys V, & Masopust D. (2019). Virus-specific memory T cells populate tumors and can be repurposed for tumor immunotherapy. Nature Communications, 10, 567.
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3

Immunofluorescent Tissue Staining Protocol

Cited 1 time
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Harvested mouse tissues were fixed in 2% paraformaldehyde for 2 h before being treated with 30% sucrose overnight for cryoprotection. The sucrose-treated tissue was embedded in OCT tissue-freezing medium and frozen in a 2-methyl butane liquid bath. Frozen blocks were processed, stained, imaged, and enumerated by QIM as described (Steinert et al., 2015 (link)). Tissues were stained with antibodies to the following markers: CD4 (GK1.5; BD Biosciences), CD90.1 (OX-7; BD Biosciences), CD45.1 (A20; BioLegend), B220 (RA3-6B2; BioLegend), CD8β (YTS156.7.7; BioLegend), and collagen-IV (goat anti-mouse polyclonal; Millipore). We counterstained with DAPI or SYTOX Green (Thermo Fisher Scientific) to detect nuclei. The following secondary antibodies were from Jackson ImmunoResearch: bovine anti-goat (polyclonal) and donkey anti-rat (polyclonal).
Beura L.K., Fares-Frederickson N.J., Steinert E.M., Scott M.C., Thompson E.A., Fraser K.A., Schenkel J.M., Vezys V, & Masopust D. (2019). CD4+ resident memory T cells dominate immunosurveillance and orchestrate local recall responses. The Journal of Experimental Medicine, 216(5), 1214-1229.
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4

Comprehensive T Cell Immunophenotyping

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Surface staining was performed using antibodies for the following markers: CD3 (145-2C11), CD4 (RM4-5), CD8 (53-6.7), CD45 (30-F11), CD90.1 (OX-7), PD-1 (RMP1-30) (BD Bioscience; eBioscience, Inc., San Diego, CA; Biolegend, San Diego, CA). Adoptively transferred Pmel-1 T cells were identified as CD3+CD8+Thy1.1+ cells, while endogenous CD8+ T cells were identified as CD3+CD8+Thy1.1−. For intracellular staining, cells were fixed and permeabilized using the Foxp3 staining buffer (eBioscience, Inc.), following manufacturer’s instructions. Samples were stained with Ki-67 (SOLA15), Foxp3 (FJK-16s) and granzyme B (GB11). For intracellular cytokine staining, cells were fixed with BD fixation and permeabilization buffer (BD Bioscience). Samples were stained with the anti-interferon-γ (IFN-γ) antibody (XMG1.2) and analyzed using LSRII and Fortessa flow cytometers (BD Bioscience). Data analysis was performed using FlowJo software (Tree Star, Inc, Ashland, OR).
Ng S.S., Nagy B.A., Jensen S.M., Hu X., Alicea C., Fox B.A., Felber B.K., Bergamaschi C, & Pavlakis G.N. (2016). Heterodimeric IL-15 Treatment Enhances Tumor Infiltration, Persistence and Effector Functions of Adoptively Transferred Tumor-specific T Cells in the Absence of Lymphodepletion. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(11), 2817-2830.
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