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Af micro nikkor 60 mm lens

Manufactured by Nikon
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Sourced in Japan

The AF Micro-NIKKOR 60 mm lens is a prime lens designed for close-up photography. It features a focal length of 60 mm and a maximum aperture of f/2.8. The lens is compatible with Nikon's autofocus system and is capable of focusing as close as 0.22 m (0.72 ft).

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Lab products found in correlation

Scmos camera, by Oxford Instruments (2 mentions) D200 camera, by Nikon (1 mentions)

Close spelling variants of this product

D5000 Nikkor lens AF-S Micro Nikkor 60 mm AF Micro Nikkor 60 mm 1:2.8 D lens AF-S Micro Nikkor 60 mm f/2.8 G ED lens Micro Nikkor 60-mm lens 60 mm lens

4 protocols using af micro nikkor 60 mm lens

1

Multimodal Imaging of Glioma Progression

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WFOM was conducted to collect simultaneous reflectance and GCaMP6f fluorescence signal as previously described in Ma et al. (2016a) (link), using high-speed strobed LED illumination (band-pass filtered to be centered at 490, 530 and 630 nm) time-locked with an sCMOS camera (Andor Zyla) acquiring at 50.25 Hz. Reflectance and fluorescence emission light from the brain was collected with a 500–640 nm bandpass filter to reject illumination wavelengths and focused using an AF Micro-NIKKOR 60 mm lens (Nikon). Webcam monitoring of the mouse was collected throughout imaging with infrared illumination.
Mice were imaged longitudinally throughout the period of tumor development, starting at 4 days post glioma cell injection, with each mouse having at least 8 imaging sessions. Each animal was head-restrained for a maximum of two hours per imaging session. Resting state recordings involved 12 to 18 recordings (180 s duration each) over the course of each imaging session. For whisker barrel stimulation, recordings were 30 s in duration (10 s pre-stimulation, 5 s whisker stimulation, 15 s post-stimulation). Stimulation was achieved using a small bar attached to a computer-controlled stepper motor positioned to brush the whiskers in an up-down motion at 20 Hz during the stimulus period. A total of 30 recordings were performed for each of the right and left whiskers per imaging session.
Montgomery M.K., Kim S.H., Dovas A., Zhao H.T., Goldberg A.R., Xu W., Yagielski A.J., Cambareri M.K., Patel K.B., Mela A., Humala N., Thibodeaux D.N., Shaik M.A., Ma Y., Grinband J., Chow D.S., Schevon C., Canoll P, & Hillman E.M. (2020). Glioma-Induced Alterations in Neuronal Activity and Neurovascular Coupling during Disease Progression. Cell reports, 31(2), 107500.
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2

Multimodal Imaging of Glioma Progression

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WFOM was conducted to collect simultaneous reflectance and GCaMP6f fluorescence signal as previously described in Ma et al. (2016a) (link), using high-speed strobed LED illumination (band-pass filtered to be centered at 490, 530 and 630 nm) time-locked with an sCMOS camera (Andor Zyla) acquiring at 50.25 Hz. Reflectance and fluorescence emission light from the brain was collected with a 500–640 nm bandpass filter to reject illumination wavelengths and focused using an AF Micro-NIKKOR 60 mm lens (Nikon). Webcam monitoring of the mouse was collected throughout imaging with infrared illumination.
Mice were imaged longitudinally throughout the period of tumor development, starting at 4 days post glioma cell injection, with each mouse having at least 8 imaging sessions. Each animal was head-restrained for a maximum of two hours per imaging session. Resting state recordings involved 12 to 18 recordings (180 s duration each) over the course of each imaging session. For whisker barrel stimulation, recordings were 30 s in duration (10 s pre-stimulation, 5 s whisker stimulation, 15 s post-stimulation). Stimulation was achieved using a small bar attached to a computer-controlled stepper motor positioned to brush the whiskers in an up-down motion at 20 Hz during the stimulus period. A total of 30 recordings were performed for each of the right and left whiskers per imaging session.
Montgomery M.K., Kim S.H., Dovas A., Zhao H.T., Goldberg A.R., Xu W., Yagielski A.J., Cambareri M.K., Patel K.B., Mela A., Humala N., Thibodeaux D.N., Shaik M.A., Ma Y., Grinband J., Chow D.S., Schevon C., Canoll P, & Hillman E.M. (2020). Glioma-Induced Alterations in Neuronal Activity and Neurovascular Coupling during Disease Progression. Cell reports, 31(2), 107500.
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3

Mammalian Foot Morphology Diversity

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The feet of 260 mammalian specimens were photographed. These represent ~5% of all described mammalian species. Specimens were from the skins collections at Liverpool World Museum and National Museums Scotland and specific specimen numbers (when present) can be found in the Supplementary Material (SupplementaryData.pdf). Types of specimens were selected to be proportional to the distribution of mammalian diversity (i.e., more species were sampled from Primates and Rodentia than Artiodactyla). All work was approved by the ethics committee at Manchester Metropolitan University (No: 10682). The objective was to photograph specimens from a range of different genera to ensure diverse examples, since it was observed that mammals from the same genus tended to have similar foot morphology (i.e., see Figure 1f and g for comparison—Leopard, Panthera pardus and Tiger, Panthera tigris). The best specimens available for each genus were selected for photographing, which were identified as the cleanest and best-preserved examples. A digital camera (D3200 with an AF Micro Nikkor 60 mm lens, Nikon, Tokyo, Japan) was positioned above the specimen on a tripod. Photographs were taken with a ruler in focus at the same level of the feet for callibration. A desk lamp was used for photographs when extra light was required.
Spurrier S., Allen T, & Grant R.A. (2022). Investigating Foot Morphology in Rock Climbing Mammals: Inspiration for Biomimetic Climbing Shoes. Biomimetics, 8(1), 8.
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4

Fossil Pinites Collection and Imaging

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All specimens used in this study (S1 Table) were borrowed from the Herbarium of the University of Tokyo (TI) and the Fossil collections of the Osaka Museum of Natural History (OSA; for details on these herbaria including contact information, see Index Herbariorum [35 ]). No other specimens were used in this study. Specimens were photographed using a D200 camera (Nikon, Tokyo, Japan) with an AF MICRO NIKKOR 60 mm lens (Nikon) under fluorescent illumination.
The holotype of Pinites fujiii stored in TI consists of a female cone, a replica of the cone, and four microscope slides mounting sectioned parts of the cone. No specimen number is assigned to the holotype, while it is registered as “holotype of Pinites fujiii”. The holotype was collected in Seto-shi, Aichi Pref., Japan (Fig 1), from the Seto Formation, but the exact locality is not available.
Other specimens, including Miki’s [30 ] specimens, are stored in OSA F. These specimens were slightly compressed mummifications collected from the Seto or Tokiguchi Formations (Fig 1).
Yamada T., Yamada M, & Tsukagoshi M. (2015). Taxonomic Revision of Pinus fujiii (Yasui) Miki (Pinaceae) and Its Implications for the Phytogeography of the Section Trifoliae in East Asia. PLoS ONE, 10(12), e0143512.
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