Log In Sign up
The largest database of trusted experimental protocols

Hrp conjugated anti rabbit igg na934

Manufactured by GE Healthcare
Visit Supplier
Sourced in United States

HRP-conjugated anti-rabbit IgG (NA934) is a secondary antibody used in immunoassays and Western blotting applications. It is a polyclonal antibody raised in goat, and is labeled with horseradish peroxidase (HRP) for detection purposes.

Automatically generated - may contain errors

Lab products found in correlation

Anti cpla2, by Cell Signaling Technology (1 mentions) Phosphatase inhibitor cocktail 3, by Merck Group (1 mentions) Chemidoc system, by Bio-Rad (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Anti phospho erk, by Cell Signaling Technology (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Anti cox 2, by Cell Signaling Technology (1 mentions) Anti tuj1 mms 435p, by Fortrea (1 mentions) Anti rap1 sc 65, by Santa Cruz Biotechnology (1 mentions) M per mammalian protein extraction reagent, by Thermo Fisher Scientific (1 mentions) Imagequant las 4000, by Fujifilm (1 mentions) Halt protease and phosphatase inhibitor single use cocktail, by Thermo Fisher Scientific (1 mentions) Mini protean tgx gel, by Bio-Rad (1 mentions) Las 4000, by Cytiva (1 mentions) A5441, by Merck Group (1 mentions) A2066, by Merck Group (1 mentions) Lumi phos wb, by Thermo Fisher Scientific (1 mentions) Rabbit anti actin antibody, by Merck Group (1 mentions)

Spelling variants of this product

NA934 (129 citations) Anti‑rabbit IgG (NA934 (3 citations)

4 protocols using hrp conjugated anti rabbit igg na934

1

Western Blot Analysis of Cellular Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed with a cell lysis buffer (Cell Signaling Technology) containing 1% Triton X-100, Protease Inhibitor Cocktail (Sigma-Aldrich), and Phosphatase Inhibitor Cocktail 3 (Sigma-Aldrich). After centrifugation at 10,000×g for 15 min, the supernatants were collected. The cell lysates were then fractionated by SDS–polyacrylamide gel electrophoresis and electroblotted onto PVDF membranes. The membranes were probed with primary antibodies and HRP-conjugated secondary antibodies. Protein was detected using the ECL system and analysed using a ChemiDoc system (Bio-Rad Laboratories, Hercules, CA, USA). The following primary antibodies were used: anti-phospho-ERK (Thr202/tyr204; #4377, RRID: AB_331775), anti-ERK (#4695, RRID: AB_390779), anti-COX2 (#4842, RRID: AB_2084968), anti-cPLA2 (#2832S, RRID: AB_2164442), anti-phospoh-cPLA2 (#2831S, RRID: AB_2164445) and anti-GAPDH (#2118, RRID: AB_561053), all from Cell Signaling Technology. HRP-conjugated anti-rabbit IgG (NA934; GE Healthcare, Chicago, IL, USA) was used as the secondary antibody.
Sadatomi D., Kono T., Mogami S, & Fujitsuka N. (2020). Weak acids induce PGE2 production in human oesophageal cells: novel mechanisms underlying GERD symptoms. Scientific Reports, 10, 20775.
+ Open protocol
+ Expand
2

Antibody Catalog for Neuronal Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-PSD-Zip70 (C-terminus) (Konno et al., 2002 (link)) antibody was described previously. The following antibodies were purchased: anti-Rap1 (sc-65, RRID:AB_632321) and anti-SPAR (sc-20846, RRID:AB_2187936) from Santa Cruz Biotechnology; anti-Rap2 (610216, RRID:AB_397613) and anti-NR1 (GluN1) (556308, RRID:AB_396353) from BD Transduction Laboratories; anti-pSer880-GluR2 (GluA2) (MABN103, RRID:AB_10850324), anti-GluR1 (GluA1) (06–306), and anti-GluR2/3 (GluA2/3) (AB1506, RRID:AB_90710) from Millipore; anti-pSer845-GluR1 (GluA1) (#8084, AB_10860773) and anti-NR2B (GluN2B) (#4207, RRID:AB_1264223) from Cell Signaling Technology; anti-NR2A (GluN2A) (3916-1, RRID:AB_10933049) from Epitomics; anti-Tuj1 (MMS-435P, RRID:AB_2313773) from Covance; anti-PDZ-GEF1 (H00009693, RRID:AB_1507065) from Abnova; HRP-conjugated anti-rabbit IgG (NA934, RRID:AB_772206) and HRP-conjugated anti-mouse IgG (NA931, RRID:AB_772210) from GE Healthcare and HRP-conjugated anti-goat IgG (HAF109, RRID:AB_357236) from R&D research systems.
Mayanagi T, & Sobue K. (2020). Social Stress-Induced Postsynaptic Hyporesponsiveness in Glutamatergic Synapses Is Mediated by PSD-Zip70-Rap2 Pathway and Relates to Anxiety-Like Behaviors. Frontiers in Cellular Neuroscience, 13, 564.
+ Open protocol
+ Expand
3

Prexasertib-Induced Chk1 Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with prexasertib for 16 hours; afterward, total protein extracts were prepared using the M-PER Mammalian Protein Extraction Reagent containing the Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (Thermo Fisher Scientific). After quantification, 10 μg of total protein was separated on Mini-PROTEAN TGX gels (4%–20%, Bio-Rad Laboratories) and transferred to polyvinylidene difluoride membranes. The primary antibodies Chk1 (2G1D5) Mouse mAb #2360, Phospho-Chk1 (Ser296) antibody #2349 (Cell Signaling Technology), and β-actin (C4; MAB1501) were used. Horseradish peroxidase (HRP)–conjugated anti-mouse IgG (NA931) and HRP-conjugated anti-rabbit IgG (NA934) were purchased from GE Healthcare. Protein bands were visualized on ImmunoStar LD (Fujifilm Wako Pure Chemical) and ImageQuant LAS-4000 (Fujifilm).
Yoshida K., Yokoi A., Yamamoto T., Hayashi Y., Nakayama J., Yokoi T., Yoshida H., Kato T., Kajiyama H, & Yamamoto Y. (2022). Aberrant Activation of Cell-Cycle–Related Kinases and the Potential Therapeutic Impact of PLK1 or CHEK1 Inhibition in Uterine Leiomyosarcoma. Clinical Cancer Research, 28(10), 2147-2159.
+ Open protocol
+ Expand
4

Western Blot Analysis of Mouse Testes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Testes from 14-days post partum (dpp) and 60-dpp mouse were homogenized with RIPA buffer: 50 mM Tris–HCl (pH 7.6), 150 mM NaCl, 1% NP40, 1 mM EDTA (pH8.0), 1 mM Na3VO4, 1 mM NaF, 1 mM PMSF, 1 μg/ml aprotinin, 1 μg/ml leupeptin, 1 μg/ml pepstatin. Following 10% SDS-PAGE, proteins were electro-transferred to a PVDF membrane (Immobilon-P; Millipore). Anti-Jagged1 goat antibody (sc-6011; Santa Cruz) or anti-actin rabbit antibody (A2066; Sigma) was used as the primary antibody with AP-conjugated anti-goat IgG (AP-5000; Vector) or HRP-conjugated anti-rabbit IgG (NA934; GE Healthcare) as the secondary antibody. Lumi-Phos WB (Thermo) and Plus-ECL (Perkin Elmer) were used to detect target proteins.
For analysis of primary cells and tubule fragments, anti-β-actin antibody (A5441; Sigma), anti-WT1 antibody (sc-192; Santa Cruz), TRA98 antibody (73-003; Bio Academia), anti-cytochrome P450 side chain cleavage enzyme (P450scc) antibody (ab1244; Chemicon), and anti-Stra8 antibody (ab49602; Abcam) were also used as the primary antibody (Supplementary Table S1).
Okada R., Hara T., Sato T., Kojima N, & Nishina Y. (2016). The mechanism and control of Jagged1 expression in Sertoli cells. Regenerative Therapy, 3, 75-81.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!