Abt 702

ABT‐702 was from Merck Millipore (116890, Shanghai). MK‐2206 was from Selleck (S7025/S1078, Shanghai, 10 μmol/L). 8(p‐Sulfophenyl)theophylline (8‐SPT) was from Absin (abs42012139, Shanghai, 100 μmol/L). DPCPX was from Abcam (ab120396, Shanghai, 100 nmol/L). MRS 1754 was from MedChemExpress (HY‐14121, Shanghai, 10 nmol/L). Primary antibodies against the following targets were obtained from the sources listed: ADK (Santa Cruz, Heidelberg, 514588), XIAP (CST, 2042), phosphor‐XIAP antibody (p‐SER87) (CST, 193315), RIP1 (CST, 3493), phosphor‐RIP1 antibody (Ser166)(CST, 31122), RIP3 (CST, 15828), phosphor‐RIP3 (Abcam, ab195117), caspase‐3 (CST, 9662), caspase‐8 (CST, 4790), caspase‐9 (CST, 9504), caspase‐12 (CST, 2202), Bcl2 (CST, 3498), Bax (CST, 2772), β‐actin (Proteintech, 6008‐1‐Ig), Akt (CST, 4685), phosphor‐Akt (Ser473) (CST, 4060), P38 (CST, 8690), phosphor‐P38 (CST, 4511), MLKL (Proteintech, 66675), phosphor‐MLKL (Abcam, ab196436). CaMKII (CST, 3362), phosphor‐CaMKII (Abcam, ab32678) and adenosine receptor A_2B (GeneTex, GTX132217). Secondary antibodies were from Cell Signaling Technology. Primary antibodies were diluted at 1:1000, and secondary antibodies were diluted at 1:5000.

Cerulein was purchased from MedChem Express (HY-A0190, Shanghai). Lipopolysaccharide (LPS) was purchased from Sigma (L2630, Shanghai). ABT702 was purchased from Merck Millipore (116890, Shanghai). ZM241385 (an adenosine A_2A receptor antagonist) and GSK2606414 (PERK inhibitor) were purchased from Selleck (S8105 and S7307, respectively, Shanghai). The following primary antibodies were purchased: ß-actin antibody (Proteintech, 66009), ADK antibody (Abcam, ab227087), RIP1 antibody (CST, 3,493), phosphor-RIP1 (Ser161) antibody (Affinity, AF7377), RIP3 antibody (CST, 15828), phosphor-RIP3 antibody (Abcam, ab195117), mouse MLKL antibody (Proteintech, 66675), rat MLKL antibody (Abcam, ab243142), phosphor-MLKL antibody (Affinity, AF7420), NF-κB p65 antibody (CST, 8242), phosphor-NF-κB p65 antibody (CST, 3,033), GRP78 Antibody (Proteintech, 11587), CHOP antibody (Proteintech, 15204), PERK antibody (Proteintech, 24390), phosphor-PERK antibody (CST, 3,179), eIF2α antibody (CST, 5324), phosphor-eIF2α antibody (CST, 3,398), CD11b antibody (Abcam, ab133357), MOMA-2 antibody (Abcam, ab33451), and amylase antibody (Santa Cruz Biotechnology, sc-46657). Secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States).