Cd38 pe cy5

Manufactured by BD

Sourced in United States

CD38-PE-Cy5.5 is a fluorochrome-conjugated antibody that binds to the CD38 antigen. It is designed for use in flow cytometry applications for the detection and identification of cells expressing the CD38 marker.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

CD38-PE (65 citations)

4 protocols using cd38 pe cy5

Hematopoietic Stem Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human CD34+ cells (Lonza and AllCells) from all sources were obtained as viable frozen states. The age of the individuals used for the profiling and treatments are as follows: FL-CD34+ (17–20 weeks gestation); CB-CD34+ (newborns); BM-CD34+ (24–36 years old). For the generation of HSC/PROG transcriptional profiles, at least three distinct lot numbers, each corresponding to independent individuals or pools of distinct individuals (of random male or female samples), were utilized to attain maximal representation. For each biological replicate, HSC and PROG populations were sorted from the same pool of cells. Cells were stained and sorted using a BD FACS Aria II cell sorter for panels of cell surface markers and dyes as indicated below.
HSC panel: CD34 PE-Cy7 (8G12; BD), CD38 PE-Cy5 (HIT2; BD), CD90 PE (5E10; BD), CD45RA-FITC (HI100; BioLegend), DAPI
PROG panel: CD34 PE-Cy7 (8G12; BD), CD38 PE-Cy5 (HIT2; BD), DAPI
Stem Cell panel: CD34 PE-Cy7 (8G12; BD), CD38 PE-Cy5 (HIT2; BD), CD90 PE (5E10; BD), CD45RA-V450 (H100; BD), CD133/1-APC (AC133; Miltenyi Biotec)

Cesana M., Guo M.H., Cacchiarelli D., Wahlster L., Barragan J., Doulatov S., Vo L.T., Salvatori B., Trapnell C., Clement K., Cahan P., Tsanov K.M., Sousa P.M., Tazon-Vega B., Bolondi A., Giorgi F.M., Califano A., Rinn J.L., Meissner A., Hirschhorn J.N, & Daley G.Q. (2018). A CLK3-HMGA2 alternative splicing axis impacts human hematopoietic stem cell molecular identity throughout development. Cell stem cell, 22(4), 575-588.e7.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following monoclonal antibodies were used in the study: CD8‐FITC, CD27‐FITC, Interferon (IFN)‐gamma‐FITC, CD24‐PE, CD80‐PE, CD197‐PE (CCR7‐PE), CD3‐PerCP, CD38‐PE‐Cy5.5, CD19‐PE‐Cy7, CD25‐PE‐Cy7, programmed death‐ligand 1 (PD‐L1)‐PE‐Cy7, TNF‐alpha‐PerCP‐Cy5.5, CD45RO‐APC, CD56‐APC, HLA‐DR‐APC, CD4‐APC‐H7, CD8‐APC‐H7, CD14‐APC‐H7, CD3‐BV421, CD69‐BV421, CD127‐BV421, CD45‐V500 (all from BD Biosciences, San Diego, CA); interleukin (IL)‐10‐PE (Biolegend, San Diego, CA), and IL‐17‐APC (R&D Systems, Minneapolis, MN).

Medina S., Sainz de la Maza S., Villarrubia N., Álvarez‐Lafuente R., Costa‐Frossard L., Arroyo R., Monreal E., Tejeda‐Velarde A., Rodríguez‐Martín E., Roldán E., Álvarez‐Cermeño J.C, & Villar L.M. (2019). Teriflunomide induces a tolerogenic bias in blood immune cells of MS patients. Annals of Clinical and Translational Neurology, 6(2), 355-363.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the study of the cell populations, the following monoclonal antibodies were used: Granulocyte/macrophage-colony stimulating factor (GM-CSF)-PE, CD197-PE (CCR7-PE), CD24-PE, Interferon (IFN)-gamma-FITC, CD14-FITC, CD8-FITC, CD27-FITC, CD8-APC-H7, CD4-APC-H7, CD56-APC, CD45RO-APC, CD3-BV421, CD127-BV421, CD45-V500 TNF-alpha-PerCP-Cy5.5, CD38-PE-Cy5.5, CD19-PE-Cy7, CD25-PE-Cy7, CD3-PerCP, (BD Biosciences, San Diego, CA, USA) and IL-17-APC (R&D Systems, Minneapolis, MN, USA).

Pérez-Pérez S., Domínguez-Mozo M.I., Alonso-Gómez A., Medina S., Villarrubia N., Fernández-Velasco J.I., García-Martínez M.Á., García-Calvo E., Estévez H., Costa-Frossard L., Alvarez-Cermeño J.C., Luque-Garcia J.L., Arroyo R., Villar L.M, & Alvarez-Lafuente R. (2020). Acetate correlates with disability and immune response in multiple sclerosis. PeerJ, 8, e10220.

+ Open protocol

+ Expand

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the study of the cell populations, the following monoclonal antibodies were used: Granulocyte/macrophage-colony stimulating factor (GM-CSF)-PE, CD197-PE (CCR7-PE), CD24-PE, Interferon (IFN)-gamma-FITC, CD14-FITC, CD16-PE-Cy7, CD8-FITC, CD27-FITC, CD8-APC-H7, CD4-APC-H7, CD4-PE-Cy7, CD123-Bv421, CD56-APC, CD56-Bv421, CD45RO-APC, CD45-APC-H7, CD3-BV421, CD127-BV421, CD127-FITC, CD45-V500 TNF-alpha-PerCP-Cy5.5, CD38-PE-Cy5.5, CD19-PE-Cy7, CD19-APC, CD25-PE-Cy7, CD3-PerCP, HLA-DR-PerCP, CD11c-PE (BD Biosciences, San Diego, CA, USA) and IL-17-APC (R&D Systems, Minneapolis, MN, USA).

Dominguez-Mozo M.I., Perez-Perez S., Villarrubia N., Costa-Frossard L., Fernandez-Velasco J.I., Ortega-Madueño I., Garcia-Martinez M.A., Garcia-Calvo E., Estevez H., Luque Garcia J.L., Torrejon M.J., Arroyo R., Villar L.M, & Alvarez-Lafuente R. (2021). Herpesvirus Antibodies, Vitamin D and Short-Chain Fatty Acids: Their Correlation with Cell Subsets in Multiple Sclerosis Patients and Healthy Controls. Cells, 10(1), 119.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!