2 aminoethyl methacrylate hydrochloride

Monohydroxy-capped poly(ethylene glycol) (PEG₁₁₃-OH) with a mean degree of polymerization, DP, of 113 (M_n = 5000 g/mol, M_w/M_n = 1.02) was purchased from Aldrich and dried at 30 °C under vacuum overnight prior to use. 2-Diethylaminoethyl methacrylate (DEA, Aldrich, 99%) and styrene (St, Aldrich, 99%) were passed through basic alumina column to remove inhibitor and then vacuum distilled from CaH₂ and stored at − 20 °C prior to use. 2, 2-Bipyridine (bpy), copper bromide (CuBr), 2-bromoisobutyryl bromide, 2-aminoethyl methacrylate hydrochloride (AMA^.HCl), d-glucono-δ-lactone, bortezomib (BTZ), and Alizarin Red S (ARS) were all purchased from J&K and directly used. Methanol, toluene, triethylamine (TEA), 1-methyl-2-pyrrolidinone (NMP), and isopropyl alcohol (IPA) were purchased from Sinopharm Group Chemical Reagent Co., Ltd., and then distilled from CaH₂ under reduced pressure. Dichloromethane, chloroform, dimethyl sulfoxide (DMSO), and other organic solvents were purchased from Sinopharm Group Chemical Reagent Co., Ltd., and directly used without further purification. Bromide terminated poly(ethylene glycol) (PEG₁₁₃-Br) macroinitiator and 2-gluconamidoethyl methacrylate (GAMA) monomer were prepared from PEG₁₁₃-OH and 2-bromoisobutyryl bromide, and AMA^.HCl and d-glucono-δ-lactone, respectively, according to literature procedures [35 (link), 36 (link)].

Dimethyldiethoxysilane (DMDES), 3-(trimethoxysilyl)propyl methacrylate (MPS), poly(ethylene glycol) methyl ether acrylate (PEGMA, M_n = 480), and 2-(dimethylamino)ethyl methacrylate (DMAEMA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). In addition, 2-aminoethyl methacrylate hydrochloride (AEMA) was supplied by J&K Scientific Co., Ltd., Beijing, China. Poly(ethylene glycol) dimethacrylate (PEGDMA) was obtained from Tokyo Chemical Industry (Tokyo, Japan). Further, 8-arm-PEG-ACLT (20 kDa) was purchased from JenKem Technology Co., Ltd., Beijing, China. Doxorubicin hydrochloride (DOX) was purchased from Aladdin (Shanghai, China). Ammonium hydroxide (NH₄OH content 28%–30%), sodium dodecyl sulfate (SDS), potassium persulfate (KPS), and ethanol were obtained from Sinopharm Chemical Reagent Co., Ltd., Beijing, China. Dulbecco’s modified Eagle’s medium (DMEM) and Dulbecco’s phosphate-buffered saline (DPBS) were purchased from Neuronbc, Beijing, China. Fetal bovine serum (FBS) and Trypsin were obtained from Gibco, Carlsbad, CA, USA.