Cerulein was purchased from MedChem Express (HY-A0190, Shanghai). Lipopolysaccharide (LPS) was purchased from Sigma (L2630, Shanghai). ABT702 was purchased from Merck Millipore (116890, Shanghai). ZM241385 (an adenosine A2A receptor antagonist) and GSK2606414 (PERK inhibitor) were purchased from Selleck (S8105 and S7307, respectively, Shanghai). The following primary antibodies were purchased: ß-actin antibody (Proteintech, 66009), ADK antibody (Abcam, ab227087), RIP1 antibody (CST, 3,493), phosphor-RIP1 (Ser161) antibody (Affinity, AF7377), RIP3 antibody (CST, 15828), phosphor-RIP3 antibody (Abcam, ab195117), mouse MLKL antibody (Proteintech, 66675), rat MLKL antibody (Abcam, ab243142), phosphor-MLKL antibody (Affinity, AF7420), NF-κB p65 antibody (CST, 8242), phosphor-NF-κB p65 antibody (CST, 3,033), GRP78 Antibody (Proteintech, 11587), CHOP antibody (Proteintech, 15204), PERK antibody (Proteintech, 24390), phosphor-PERK antibody (CST, 3,179), eIF2α antibody (CST, 5324), phosphor-eIF2α antibody (CST, 3,398), CD11b antibody (Abcam, ab133357), MOMA-2 antibody (Abcam, ab33451), and amylase antibody (Santa Cruz Biotechnology, sc-46657). Secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States).
Grp78 antibody
Manufactured by Proteintech
Sourced in China
The GRP78 Antibody is a primary antibody that recognizes the GRP78 (glucose-regulated protein 78) protein. GRP78 is a chaperone protein that plays a crucial role in the endoplasmic reticulum (ER) stress response. This antibody can be used for the detection and analysis of GRP78 in various biological samples through techniques such as Western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Chop antibody, by Proteintech (2 mentions)
Amylase antibody, by Santa Cruz Biotechnology (1 mentions)
Cd11b antibody, by Abcam (1 mentions)
Moma 2 antibody, by Abcam (1 mentions)
Cerulein, by MedChemExpress (1 mentions)
Zm 241385, by Selleck Chemicals (1 mentions)
Gsk2606414, by Selleck Chemicals (1 mentions)
Abt 702, by Merck Group (1 mentions)
Perk antibody, by Proteintech (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Lactate dehydrogenase, by Beyotime (1 mentions)
Dab horseradish peroxidase color development kit, by Beyotime (1 mentions)
Cleaved caspase 3, by Proteintech (1 mentions)
Sybr green premix, by Takara Bio (1 mentions)
Alt assay kit c009 2 1, by Nanjing Jiancheng (1 mentions)
Ast assay kit c010 2 1, by Nanjing Jiancheng (1 mentions)
Peg300, by Merck Group (1 mentions)
Pe annexin 5 apoptosis detection kit, by BD (1 mentions)
Tunel apoptosis assay kit, by Beyotime (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
3 protocols using grp78 antibody
1
Cerulein-Induced Pancreatitis Signaling
2
Investigating ER Stress-Mediated Apoptosis
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OEA, DMSO, PEG300, and Tween-80 were purchased from Sigma Chemical (USA). Tunicamycin (TM) was purchased from MCE (NJ, USA). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Viva Cell Biosciences (China). RIPA Lysis Buffer, PMSF, Cell Counting Kit-8 (C0037), DAB Horseradish Peroxidase Color Development Kit, lactate dehydrogenase (LDH), Cytotoxicity Assay Kit, and TUNEL Apoptosis Assay Kit (C1086) were purchased from Beyotime (China). PPARα antibody (Cat: 15540-1-AP), Grp78 antibody (Cat: 11587-1-AP), CHOP antibody (Cat: 15204-1-AP), Caspase 12 antibody (Cat: 55238-1-AP), cleaved-Caspase 3 (Cat: 19677-1-AP), Bcl2 (Cat: 12789-1-AP) antibody, Bax antibody (Cat: 50599-2-Ig), β-actin antibody (Cat: 66009-1-Ig), and HRP-conjugated goat anti-mouse/rabbit IgG (SA00001-1/2) were purchased from Proteintech (China). ALT assay kit (C009-2-1) and AST assay kit (C010-2-1) were purchased from Jiancheng Bioengineering Institute (Nanjing, China). Annexin V PE Apoptosis Detection Kit (559763) was purchased from BD Pharmingen (NJ, USA). Lipofectamine™3000 and TRIzol were purchased from Invitrogen. SYBR Green® Premix was purchased from TaKaRa. Chemiluminescent kit was purchased from NCM Biotech (Suzhou, China).
3
Immunofluorescence Analysis of GRP78 Expression
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HOS cells in the logarithmic phase of growth were seeded in a 6-well plate with 25 mm cell slides. After processing the cells according to experimental groups, the cells were fixed with 4% paraformaldehyde for 30 min, washed with PBS three times, lysed with 0.1% Triton X-100 (Beyotime Biotechnology Co., Ltd) for 15 min, blocked with goat anti-rabbit serum for 40 min, and incubated with rabbit polyclonal GRP78 antibody (ProteinTech Group, Inc. Wuhan, China) (diluted 1:100) overnight at 4°C. Cy3-labeled goat anti-rabbit IgG fluorescent secondary antibody (Bioss Biotechnology Co., Ltd, Beijing, China) was added to the cells in the dark and incubated for 40 min at room temperature. The cells were washed three times with PBS, the nuclei were stained with DAPI for 5 min, and the cells were washed with PBS to remove the residual DAPI. Then, the slides were mounted and observed with a fluorescence microscope (Nikon). Fluorescence intensity analysis was performed with ImageJ 1.52e.
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