Anti litaf

The protein samples were resolved by SDS-polyacrylamide gel electrophoresis (PAGE), electroph oretically transferred to PVDF membranes (Millipore) and blocked in 5% BSA or nonfat milk in PBS/Tween-20. Antibodies specific for BCL6, Cleaved-PARP, Cleaved-caspase 3, Bcl-XL, Bax and cytochrome c were purchased from Cell Signaling Technology. The anti-LITAF, anti-actin, anti-HA and anti-c-Myc antibodies were purchased from Santa Cruz Biotechnology. The experiments for the immunoblotting were performed at least three times.

TAT1 anti-tubulin was a gift from Keith Gull (University of Oxford). The following commercial antibodies were used. Mouse: anti-LITAF (Santa Cruz); anti-transferrin receptor (Zymed); anti-Strep-Tag (Novagen); anti-CD63 (Millipore); anti-EEA1 (early endosome antigen 1; BD Biosciences); anti-LAMP1 (lysosome-associated membrane protein 1; Developmental Studies Hybridoma Bank, University of Iowa); anti-OPG2 (opsin tag containing two glycosylation sites) was described previously [29 (link)]. Rabbit: anti-TorsinA was a gift from Lisa Swanton (University of Manchester); anti-V5 (Abcam); anti-EEA1, anti-LAMP1 (Cell Signaling). Sheep: anti-GFP was an in-house antibody generated against GST-GFP. Fluorescent secondary antibodies for IF and for Licor immunoblotting were from Jackson ImmunoResearch Laboratories (PA, USA).