Anaeropack sachet

The strains used in this study were grown on trypticase soy agar supplemented with 5% sheep blood (BD, Franklin Lakes, NJ, USA) for 5 days at 37 °C under anaerobic conditions created in an anaerobic jar with an AnaeroPack sachet (Mitsubishi Gas Chemical, Tokyo, Japan). The colonies were observed, and Gram stain was performed using Gram Stain Kit (Stabilized) (BD).

Eight strains of genus Cutibacterium and seven strains of genus Staphylococcus were obtained from Japan Collection of Microorganisms (JCM); National Collection of Type Cultures (NCTC), United Kingdom; Culture Collection of University of Goteborg (CCUG), Sweden; and Monash University, Melbourne, Australia (Table 1). The full genome sequences of these strains are available from the DDBJ/EMBL/GenBank public database. For Cutibacterium species, strains were grown on trypticase soy agar supplemented with 5% sheep blood (BD, Franklin Lakes, NJ, USA) for 7 days under anaerobic conditions (0% O₂/10% CO₂), created in an anaerobic jar with an AnaeroPack sachet (Mitsubishi Gas Chemical, Tokyo, Japan) at 37 °C. Moreover, for C. acnes subsp. acnes JCM 6425^T, C. acnes subsp. acnes JCM 18918, and C. acnes subsp. elongatum JCM 18919^T, cultivation was performed under a normal atmosphere (21% O₂/0.04% CO₂), and different oxygen pressures for culture were established by the hypoxia workstation Invivo₂ (Baker Ruskinn, Bridgend, Wales, UK) for 12% O₂/9% CO₂, 6% O₂/15% CO₂ and 2% O₂/19% CO₂. For Staphylococcus species, strains were grown on trypticase soy agar supplemented with 5% sheep blood (BD) for 2 days under air (21% O₂/0.04% CO₂).