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Chromafil xtra ptfe 20 25

Manufactured by Macherey-Nagel
Sourced in Germany

Chromafil Xtra PTFE-20/25 is a syringe filter made of polytetrafluoroethylene (PTFE) with a pore size of 20/25 mm. It is designed for the filtration of samples prior to analysis.

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2 protocols using chromafil xtra ptfe 20 25

1

Phytochemical Analysis of Plant Extracts

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For phytochemical analysis, the husk powders were first extracted by hexane (non-polar extract) and then air-dried plant residues were extracted by ethanol and bi-distilled water (polar extract). The extraction process steps of polar and apolar compounds were the same, and powder to solvent ratio was 1:20 (both for hexane and ethanol 80%). Briefly, the mixtures of plant material and solvent were first shaken well for 5 min using a vortex mixer (TOP-MIX 94,500; Heidolph, Schwabach, Germany), and then sonicated in iced water (Fisherbrand S-Series, ThermoFisher, Milan, Italy) for 60 min. Centrifugation (Z36 HK, Hermle, Germany) at 13,540× g for 10 min was performed to accelerate the extraction of compounds. For polar compounds, a 0.2 µm polyamide filter (Chromafil AO-20/25; Macherey-Nagel, Düren, Germany), and for apolar compounds, a 0.2 µm polytetrafluoroethylene filter (Chromafil Xtra PTFE-20/25 Macherey-Nagel, Duren, Germany) was used. The resultant polar and non-polar extracts were then transferred to vials and stored at −32 °C until further analysis [21 (link)]. 2.4. Qualitative Analysis of Apolar Extract by GC-MS
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2

PMMA Film Fabrication Protocol

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The method followed that described by Le et al. [34] . PMMA sheets were cut into small pieces and dissolved in toluene at concentration ranging from 0.1 to 0.5% (w/v) to make the raw PMMA solution. The solution was sonicated for 15 min to dissolve PMMA pieces. The PMMA solution was then filtered through a PTFE filter (pore size 0.2 µm) (Chromafil Xtra PTFE-20/25 Macherey-Nagel, Duren, Germany) to eliminate the precipitates and dust particles. 0.25% (w/v) PMMA solution was then spin coated onto Aucoated glass slides or silicon wafers at 2000 rpm for 45 s. The acceleration and deceleration from 0 rpm to the desired spin speed and from spin speed to 0 rpm took place in 5 s. The films were first cured at room temperature over-night and then cured in an oven for 1h at 80 o C. These parameters resulted in very smooth and uniform PMMA films on both Aucoated glass slides and silicon wafers.
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