Chromafil xtra ptfe 20 25

For phytochemical analysis, the husk powders were first extracted by hexane (non-polar extract) and then air-dried plant residues were extracted by ethanol and bi-distilled water (polar extract). The extraction process steps of polar and apolar compounds were the same, and powder to solvent ratio was 1:20 (both for hexane and ethanol 80%). Briefly, the mixtures of plant material and solvent were first shaken well for 5 min using a vortex mixer (TOP-MIX 94,500; Heidolph, Schwabach, Germany), and then sonicated in iced water (Fisherbrand S-Series, ThermoFisher, Milan, Italy) for 60 min. Centrifugation (Z36 HK, Hermle, Germany) at 13,540× g for 10 min was performed to accelerate the extraction of compounds. For polar compounds, a 0.2 µm polyamide filter (Chromafil AO-20/25; Macherey-Nagel, Düren, Germany), and for apolar compounds, a 0.2 µm polytetrafluoroethylene filter (Chromafil Xtra PTFE-20/25 Macherey-Nagel, Duren, Germany) was used. The resultant polar and non-polar extracts were then transferred to vials and stored at −32 °C until further analysis [21 (link)]. 2.4. Qualitative Analysis of Apolar Extract by GC-MS