NFκB RE plasmid was transfected in A549-LV cells and the luciferase reporter assay was done according to Nano-Glo Luciferase Assay System protocol (Promega). TNFα was used as an activator and in some cases QNZ and TRAF2 siRNAs were also used.
Nano glo luciferase assay system protocol
Manufactured by Promega
The Nano-Glo Luciferase Assay System is a bioluminescent reporter assay used to quantify the activity of luciferase enzymes in biological samples. The assay utilizes a engineered luciferase enzyme and optimized substrate to produce a bright, stable luminescent signal that can be measured using a luminometer or other light detection instrument.
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Lab products found in correlation
2 protocols using nano glo luciferase assay system protocol
1
NFκB Luciferase Assay in A549-LV Cells
2
Luciferase Assay for S. aureus
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Overnight cultures of S. aureus strains were diluted to an OD600 of 0.05 and grown for 90 min in the presence of 100 ng/ml anhydrotetracycline (Atet). Strains were normalised to an OD600 of 0.1 and luciferase activity measured according to the Nano-Glo Luciferase Assay System Protocol (Promega).
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