Maxq420hp incubator shaker

Chlamydomonas wild type strain 4A+ (CC- 4051 4A+ mt+) was maintained in the lab on TAP agar medium in dim light intensities (15-20 µmol m
^-2s
^-1) at 22°C (room temperature). Standard TAP medium recipe can be found at the website of
Chlamydomonas Resource Center. Hutner’s trace element solution is an ingredient in the TAP medium. Hutner’s trace element recipe can also be found on the
Chlamydomonas Resource Center website. Our lab’s TAP medium recipe is slightly different from the standard one and can be found at
https://doi.org/10.17504/protocols.io.bgzujx6w^{41 (link)}. Liquid 4A+ cultures were grown in liquid TAP medium under low light (50-60 µmol m
^-2s
^-1) for 3 days on a New Brunswick Scientific Excella E5 platform shaker (Enfield, CT) at 150 rpm for aeration. CC4533 bacterial strain stock was maintained in the lab under dim light (15-20 µmol m
^-2s
^-1) at 22°C on either TAP or LB agar medium. Liquid cultures of CC4533 were grown in culture tubes in 3 mL of TAP medium at 22°C on a MaxQ420HP incubator shaker (Thermo Fisher Scientific, Waltham, MA) at 200 rpm for aeration. Light intensities were measured using a LI-250A light meter (LI-COR, Inc., Lincoln, NE).

We used the
Chlamydomonas wild type strain 4A+ (CC- 4051 4A+ mt+) strain in our antibiotic-sensitivity testing experiments. 4A+ strain was maintained on TAP agar medium in dim light intensities (15-20 µmol m
^-2s
^-1) at 22°C. Liquid 4A+ cultures were grown in 50 mL flasks in 15 mL of TAP in low light (50-80 µmol m
^-2s
^-1) for 3 days. LMJ bacterial strain was maintained in the lab under dim light at 22°C on either TAP or LB agar medium plates. Liquid cultures of LMJ were grown in culture tubes in 3 mL of TAP or LB medium. 4A+ and LMJ liquid cultures were shaken at 150 rpm for aeration on a MaxQ420HP incubator shaker (Thermo Fisher Scientific, Waltham, MA). Light intensities were measured using a LI-250A light meter (LI-COR, Inc., Lincoln, NE).
The detailed protocol utilized to produce the TAP and TP medium used in this article is available from Protocols.io:
https://doi.org/10.17504/protocols.io.bgzujx6w.