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Blt 1

Manufactured by MedChemExpress
Sourced in United States

BLT-1 is a piece of laboratory equipment designed for analytical and preparative liquid chromatography. It features a high-performance liquid chromatography (HPLC) system with automated injection, column selection, and fraction collection capabilities.

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2 protocols using blt 1

1

Comprehensive Chemical Library for Cell Experiments

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Chemicals purchased included Hydroxychloroquine (HCQ) Sulfate (Spectrum Chemicals, 747–36-4); DC661 (Selleckchem, S8808); Methyl-Beta-Cyclodextrin (332615, Sigma); Cholesterol-Water Soluble (C4951, Sigma); Simvastatin (10010344, Cayman Chemical); BLT-1 (HY-116767, MedChemExpress); Myriocin (63150–5, Cayman Chemical); Fumonisin B1 (62580–1, Cayman Chemical); Siramesine hydrochloride (6740, Tocris Bioscience); Genz-123346 (BioVision, B2286); Eliglustat hemitartarate (HY-14885A, MedChemExpress LLC); Eliglustat hemitartarate (21487, Cayman Chemical); SBI-0206965 (18477, Cayman Chemical); SAR-405 (16979, Cayman Chemical) and LysoSensor Green DND-189 (Thermo Fisher; L7535). ST1074 was synthesized by Holger Stark and Aleksandra Zivkovic (21 (link)).
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2

IRBP-driven EAU suppression by APOA1

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T cells were separated from the DLNs and spleens of EAU mice on day 14 after immunization. The isolated T cells were stimulated with IRBP1-20 (10 µg/mL) in the presence of APOA1 (10 µM) or vehicle for 72 hours. For some groups, blocking of lipid transport-1 (BLT-1, 2 µM, MedChemExpress, Monmouth Junction, NJ, USA) were performed. Next, cells were washed with PBS twice, and 2 × 107 cells were transferred back to each naive C57BL/6J mouse. All mice were evaluated for retinal inflammation two weeks later.
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