To prove intestinal flora regulation on mucosal structure, 12 D280 ZN Hens were divided into two groups, one control and one multiple‐antibiotic feeding group. In the control group, hens were fed with a basal diet and free access to water. However, in the multiple‐antibiotics group, hens were supplied with water containing Ampicillin Sodium (1 g l−1 water, MB1378; Meilunbio, Dalian, China), Neomycin Sulfate (1 g l−1 water, MB1716; Meilunbio), Metronidazole (1 g l−1 water, MB2200; Meilunbio, Dalian, China) and Vancomycin HCl (0.5 g l−1 water, MB1260; Meilunbio) for five consecutive days. On the sixth day, all the hens were killed after anaesthesia. The gut lumen content was then collected sterilely, further used for Colony‐Forming Units (CFUs) counting and 16S rRNA gene sequencing. Meanwhile, the intestinal tissue and intestinal crypt were collected for morphology, mRNA transcription and protein expression assays.
Ampicillin sodium
Manufactured by Meilun
Sourced in China
Ampicillin Sodium is a powdered form of the antibiotic ampicillin. It is a broad-spectrum penicillin-class antibiotic that can be used in laboratory settings for a variety of applications.
Automatically generated - may contain errors
2 protocols using ampicillin sodium
1
Antibiotic Impact on Gut Microbiome and Morphology
2
Antibiotic Administration in Neonatal Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6 mice were obtained from Chongqing Medical University and housed in specific pathogen free (SPF) conditions. Female and male mice were caged together at a 1:2 ratio to obtain pregnant mice. SPF neonatal C57BL/6 J pups were given antibiotics via gavage on postnatal day 3. Antibiotics were administered at 09:00 am daily for 4 consecutive days of treatment at 0.03 mL/g/day. The antibiotic cocktails were: ampicillin sodium (25 mg/kg), metronidazole (50 mg/kg), piperacillin sodium tazobactam sodium (100 mg/kg), and vancomycin (10 mg/kg) (all Meilunbio, Dalian, China) [24 (link), 25 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!