Nanodrop one microvolume uv vis spectrophotometry

Seventy-eight strains of presumably 12 Beauveria species from the BIOTEC culture collection (BCC) and ARS collection of entomopathogenic fungal cultures (ARSEF), including the ex-type strains of known species and strains previously identified as B. asiatica or B. bassiana as well as some unidentified strains following Khonsanit et al. (2020) , were used in this study (Table S1). The strains were cultured in 50 mL of potato dextrose broth and incubated at 25 °C for a week. Fungal mycelia were harvested by filtering with a sterilised nylon mesh and washed with ethylene diamine tetraacetic acid (EDTA) and distilled water. DNA extraction was done using a cetrimonium bromide (CTAB)-based method following Kobmoo et al. (2019) (link); the DNAs were purified using the high pure PCR template preparation kit (Roche). The quality and quantity of DNA were verified using Nanodrop^TM One Microvolume UV-Vis Spectrophotometry (Thermo Fisher) and an electrophoresis on 0.8 % agarose gel at 100 V for 1/2 h.

Seventy-eight strains of presumably 12 Beauveria species from the BIOTEC culture collection (BCC) and ARS collection of entomopathogenic fungal cultures (ARSEF), including the ex-type strains of known species and strains previously identified as B. asiatica or B. bassiana as well as some unidentified strains following Khonsanit et al. (2020) , were used in this study (Table S1). The strains were cultured in 50 mL of potato dextrose broth and incubated at 25 °C for a week. Fungal mycelia were harvested by filtering with a sterilised nylon mesh and washed with ethylene diamine tetraacetic acid (EDTA) and distilled water. DNA extraction was done using a cetrimonium bromide (CTAB)-based method following Kobmoo et al. (2019) (link); the DNAs were purified using the high pure PCR template preparation kit (Roche). The quality and quantity of DNA were verified using Nanodrop^TM One Microvolume UV-Vis Spectrophotometry (Thermo Fisher) and an electrophoresis on 0.8 % agarose gel at 100 V for 1/2 h.